EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
---|
3.2.1.70 | 37 |
- |
purified recombinant His6-tagged enzyme, pH 6.0, inactivation rate constant is 0.0001/min, T1/2 at 37°C is 4.8 days |
732004 |
3.2.1.70 | 40 |
- |
15 min, pH 6.0, stable below |
664233 |
3.2.1.70 | 46 |
- |
the enzyme activity drops to 50% in 5 min at 46°C at 0-1 mM Ca2+, but the presence of 2 mM Ca2+ prolongs this to 30 min and 3-30 mM Ca2+ stabilizes the enzyme so that it retains 70% activity or higher even after 60 min incubation at 46°C |
714533 |
3.2.1.70 | 50 |
- |
20 min, 80% of activity |
657405 |
3.2.1.70 | 50 |
- |
purified recombinant His6-tagged enzyme, pH 6.0, inactivation rate constant is 0.0152/min, T1/2 at 50°C is 46 min |
732004 |
3.2.1.70 | 55 |
- |
10 min, less than 20% loss of activity |
665766 |
3.2.1.70 | 55 |
- |
not stable above |
208767, 208769, 208775 |
3.2.1.70 | 60 |
- |
pH 7.2, stable up to, wild type and several deletion mutants |
654436 |