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Literature summary for 3.2.1.70 extracted from

  • Saburi, W.; Mori, H.; Saito, S.; Okuyama, M.; Kimura, A.
    Structural elements in dextran glucosidase responsible for high specificity to long chain substrate (2006), Biochim. Biophys. Acta, 1764, 688-698.
    View publication on PubMed
Search for more literature for 3.2.1.70

Cloned(Commentary)

Cloned (Comment) Organism
wild-type and mutant enzymes produced in Escherichia coli Streptococcus mutans

Protein Variants

Protein Variants Comment Organism
W238A mutant shows lower preference for kojibiose, nigerose, and sucrose and higher preference for maltooligosaccharides, trehalose, and p-nitrophenyl alpha-D-glucoside. Altough trehalose is not a substrate for the wild-type, the mutant enzyme hydrolyzes trehalose Streptococcus mutans
W238N mutant shows lower preference for kojibiose, nigerose, and sucrose and higher preference for maltooligosaccharides, trehalose, and p-nitrophenyl alpha-D-glucoside. Altough trehalose is not a substrate for the wild-type, the mutant enzyme hydrolyzes trehalose Streptococcus mutans
W238P mutant shows lower preference for kojibiose, nigerose, and sucrose and higher preference for maltooligosaccharides, trehalose, and p-nitrophenyl alpha-D-glucoside. Altough trehalose is not a substrate for the wild-type, the mutant enzyme hydrolyzes trehalose Streptococcus mutans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.549
-
 p-nitrophenyl alpha-D-glucoside pH 6.0, 37°C, wild-type enzyme Streptococcus mutans
1.03
-
 panose pH 6.0, 37°C, wild-type enzyme Streptococcus mutans
2.2
-
 panose pH 6.0, 37°C, mutant enzyme W238N Streptococcus mutans
3.17
-
 isomaltotriose pH 6.0, 37°C, wild-type enzyme Streptococcus mutans
4.22
-
 panose pH 6.0, 37°C, mutant enzyme W238A Streptococcus mutans
4.96
-
 isomaltotriose pH 6.0, 37°C, mutant enzyme W238N Streptococcus mutans
5.1
-
 isomaltopentaose pH 6.0, 37°C, wild-type enzyme Streptococcus mutans
5.58
-
 isomaltotetraose pH 6.0, 37°C, wild-type enzyme Streptococcus mutans
6.41
-
 panose pH 6.0, 37°C, mutant enzyme W238P Streptococcus mutans
7.77
-
 isomaltotetraose pH 6.0, 37°C, mutant enzyme W238N Streptococcus mutans
7.88
-
 isomaltohexaose pH 6.0, 37°C, wild-type enzyme Streptococcus mutans
8.91
-
 isomaltose pH 6.0, 37°C, mutant enzyme W238N Streptococcus mutans
9.35
-
 isomaltose pH 6.0, 37°C, wild-type enzyme Streptococcus mutans
9.92
-
 p-nitrophenyl alpha-D-glucoside pH 6.0, 37°C, mutant enzyme W238N Streptococcus mutans
10.2
-
 isomaltotriose pH 6.0, 37°C, mutant enzyme W238P Streptococcus mutans
11.4
-
 isomaltotriose pH 6.0, 37°C, mutant enzyme W238A Streptococcus mutans
12
-
 isomaltoheptaose pH 6.0, 37°C, wild-type enzyme Streptococcus mutans
15.1
-
 isomaltopentaose pH 6.0, 37°C, mutant enzyme W238N Streptococcus mutans
15.8
-
 isomaltose pH 6.0, 37°C, mutant enzyme W238A Streptococcus mutans
16.1
-
 isomaltotetraose pH 6.0, 37°C, mutant enzyme W238P Streptococcus mutans
19
-
 p-nitrophenyl alpha-D-glucoside pH 6.0, 37°C, mutant enzyme W238A Streptococcus mutans
19.9
-
 isomaltohexaose pH 6.0, 37°C, mutant enzyme W238N Streptococcus mutans
20.5
-
 isomaltose pH 6.0, 37°C, mutant enzyme W238P Streptococcus mutans
20.9
-
 isomaltotetraose pH 6.0, 37°C, mutant enzyme W238A Streptococcus mutans
24.6
-
 isomaltopentaose pH 6.0, 37°C, mutant enzyme W238P Streptococcus mutans
26.9
-
 isomaltoheptaose pH 6.0, 37°C, mutant enzyme W238N Streptococcus mutans
27.1
-
 isomaltohexaose pH 6.0, 37°C, mutant enzyme W238P Streptococcus mutans
38.3
-
 isomaltopentaose pH 6.0, 37°C, mutant enzyme W238A Streptococcus mutans
41.4
-
 p-nitrophenyl alpha-D-glucoside pH 6.0, 37°C, mutant enzyme W238P Streptococcus mutans
43.6
-
 isomaltoheptaose pH 6.0, 37°C, mutant enzyme W238P Streptococcus mutans
76.9
-
 isomaltohexaose pH 6.0, 37°C, mutant enzyme W238A Streptococcus mutans
81.7
-
 isomaltoheptaose pH 6.0, 37°C, mutant enzyme W238A Streptococcus mutans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
63000
-
 wild-type enzyme, gel filtration Streptococcus mutans

Organism

Organism UniProt Comment Textmining
Streptococcus mutans Q2HWU5
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-D-glucoside + H2O 377% of the activity with isomaltose, wild-type enzyme Streptococcus mutans 4-nitrophenol + D-glucose
-
 ?
dextran + H2O
-
 Streptococcus mutans alpha-D-glucose
-
 ?
isomaltoheptaose + H2O 148% of the activity with isomaltose, wild-type enzyme Streptococcus mutans D-glucose
-
 ?
isomaltohexaose + H2O 148% of the activity with isomaltose, wild-type enzyme Streptococcus mutans D-glucose
-
 ?
isomaltopentaose + H2O 148% of the activity with isomaltose, wild-type enzyme Streptococcus mutans D-glucose
-
 ?
isomaltose + H2O
-
 Streptococcus mutans 2 D-glucose
-
 ?
isomaltotetraose + H2O 148% of the activity with isomaltose, wild-type enzyme Streptococcus mutans D-glucose
-
 ?
isomaltotriose + H2O 221% of the activity with isomaltose, wild-type enzyme Streptococcus mutans D-glucose
-
 ?
kojibiose + H2O 4.02% of the activity with isomaltose, wild-type enzyme Streptococcus mutans 2 D-glucose
-
 ?
maltose + H2O 0.0135% of the activity with isomaltose, wild-type enzyme Streptococcus mutans 2 D-glucose
-
 ?
maltotetraose + H2O 0.0108% of the activity with isomaltose, wild-type enzyme Streptococcus mutans maltotriose + D-glucose
-
 ?
maltotriose + H2O 0.146% of the activity with isomaltose, wild-type enzyme Streptococcus mutans maltose + D-glucose
-
 ?
additional information no activity with trehalose Streptococcus mutans ?
-
 ?
nigerose + H2O 1.99% of the activity with isomaltose, wild-type enzyme Streptococcus mutans 2 D-glucose
-
 ?
panose + H2O 417% of the activity with isomaltose, wild-type enzyme Streptococcus mutans D-glucose
-
 ?
sucrose + H2O 4.29% of the activity with isomaltose, wild-type enzyme Streptococcus mutans D-fructose + D-glucose
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 63000, wild-type enzyme, SDS-PAGE Streptococcus mutans

Synonyms

Synonyms Comment Organism
dextran glucosidase
-
 Streptococcus mutans
SMDG
-
 Streptococcus mutans

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 15 min, pH 6.0, stable below Streptococcus mutans

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.04 1.97 isomaltotetraose pH 6.0, 37°C, mutant enzyme W238N Streptococcus mutans
0.04 1.97 isomaltopentaose pH 6.0, 37°C, mutant enzyme W238N Streptococcus mutans
3 6 isomaltohexaose pH 6.0, 37°C, mutant enzyme W238A Streptococcus mutans
7.8
-
 isomaltohexaose pH 6.0, 37°C, mutant enzyme W238N Streptococcus mutans
8.88
-
 isomaltose pH 6.0, 37°C, mutant enzyme W238N Streptococcus mutans
8.96
-
 isomaltotetraose pH 6.0, 37°C, mutant enzyme W238N Streptococcus mutans
9.21
-
 isomaltopentaose pH 6.0, 37°C, mutant enzyme W238N Streptococcus mutans
9.81
-
 isomaltoheptaose pH 6.0, 37°C, mutant enzyme W238N Streptococcus mutans
12.2
-
 isomaltotriose pH 6.0, 37°C, mutant enzyme W238N Streptococcus mutans
22.3
-
 isomaltotetraose pH 6.0, 37°C, mutant enzyme W238A Streptococcus mutans
22.4
-
 panose pH 6.0, 37°C, mutant enzyme W238N Streptococcus mutans
22.6
-
 isomaltopentaose pH 6.0, 37°C, mutant enzyme W238A Streptococcus mutans
33.1
-
 isomaltotriose pH 6.0, 37°C, mutant enzyme W238A Streptococcus mutans
33.2
-
 isomaltoheptaose pH 6.0, 37°C, mutant enzyme W238A Streptococcus mutans
34.5
-
 isomaltose pH 6.0, 37°C, mutant enzyme W238A Streptococcus mutans
39.6
-
 isomaltohexaose pH 6.0, 37°C, mutant enzyme W238P Streptococcus mutans
42.1
-
 panose pH 6.0, 37°C, mutant enzyme W238A Streptococcus mutans
42.4
-
 isomaltopentaose pH 6.0, 37°C, mutant enzyme W238P Streptococcus mutans
52.5
-
 isomaltotetraose pH 6.0, 37°C, mutant enzyme W238P Streptococcus mutans
56.5
-
 isomaltoheptaose pH 6.0, 37°C, mutant enzyme W238P Streptococcus mutans
61.4
-
 p-nitrophenyl alpha-D-glucoside pH 6.0, 37°C, mutant enzyme W238N Streptococcus mutans
77.5
-
 isomaltotriose pH 6.0, 37°C, mutant enzyme W238P Streptococcus mutans
79.8
-
 isomaltose pH 6.0, 37°C, mutant enzyme W238P Streptococcus mutans
121
-
 panose pH 6.0, 37°C, mutant enzyme W238P Streptococcus mutans
202
-
 p-nitrophenyl alpha-D-glucoside pH 6.0, 37°C, mutant enzyme W238A Streptococcus mutans
235
-
 p-nitrophenyl alpha-D-glucoside pH 6.0, 37°C, wild-type enzyme Streptococcus mutans
401
-
 isomaltohexaose pH 6.0, 37°C, wild-type enzyme Streptococcus mutans
419
-
 isomaltopentaose pH 6.0, 37°C, wild-type enzyme Streptococcus mutans
449
-
 isomaltoheptaose pH 6.0, 37°C, wild-type enzyme Streptococcus mutans
483
-
 isomaltose pH 6.0, 37°C, wild-type enzyme Streptococcus mutans
499
-
 isomaltotetraose pH 6.0, 37°C, wild-type enzyme Streptococcus mutans
538
-
 panose pH 6.0, 37°C, wild-type enzyme Streptococcus mutans
541
-
 isomaltotriose pH 6.0, 37°C, wild-type enzyme Streptococcus mutans
1202
-
 p-nitrophenyl alpha-D-glucoside pH 6.0, 37°C, mutant enzyme W238P Streptococcus mutans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
 Streptococcus mutans

pH Stability

pH Stability pH Stability Maximum Comment Organism
5.5 8.4 4°C, stable for 18 h Streptococcus mutans