EC Number |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
---|
1.5.5.2 | -999 |
- |
enzyme unfolding experiments using recombinant MBP-tagged enzyme in 20 mM Hepes, pH 8.0, at 60-95°C, the MBP-tagged enzyme unfolds at lower temperature around 56°C due to unfolding of the MBP tag |
742162 |
1.5.5.2 | 45 |
- |
50% loss of activity of wild-type PutA86-669 after 1 h, 50% loss of of L432P mutant form of PutA86-669 after 8 min |
658033 |
1.5.5.2 | 70 |
- |
10 min, about 90% of the activity remains |
658668 |
1.5.5.2 | 70 |
- |
10 min, fully active |
392570 |
1.5.5.2 | 70 |
- |
the alpha4beta4-type PDHbeta retains more than 80% of its activity after heating at 70°C for 20 min |
741732 |
1.5.5.2 | 70 |
- |
the alphabetagammadelta-type PDHbeta nearly loses all of its activity during incubation at 70°C for 20 min |
741732 |
1.5.5.2 | 70 |
- |
the alphabetagammadelta-type PDHbeta retains 100% of activity during incubation at 70°C for 20 min |
741732 |
1.5.5.2 | 70 |
- |
the alphabetagammadelta-type PDHbeta retains 68% of activity during incubation at 70°C for 20 min |
741732 |
1.5.5.2 | 70 |
- |
the alphabetagammadelta-type PDHbeta retains 76% of activity during incubation at 70°C for 20 min |
741732 |
1.5.5.2 | 80 |
- |
120 min, 40% of activity remains |
392570 |