Gene and primary structures of dye-linked L-proline dehydrogenase from the hyperthermophilic archaeon Thermococcus profundus show the presence of a novel heterotetrameric amino acid dehydrogenase complex

Kawakami, R.; Sakuraba, H.; Ohshima, T.; Extremophiles 8, 99-108 (2004)

Data extracted from this reference:

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

2.5

-

L-proline

-

Thermococcus profundus

Metals/Ions

Commentary

Organism

Structure

Iron

the alpha-subunit contains the [2Fe-2S] iron-sulfur flavoprotein. The gamma subunit is a typical [8Fe-8S] ferredoxin

Thermococcus profundus

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

Substrate Product ID

L-proline + 2,6-dichlorophenolindophenol + H2O

-

658668

Thermococcus profundus

(S)-1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol

-

-

-

?

L-proline + ferricyanide + H2O

-

658668

Thermococcus profundus

(S)-1-pyrroline-5-carboxylate + ferrocyanide

-

-

-

?

Cofactor

Commentary

Organism

Structure

FAD

1.96 mol per mol of enzyme complex

Thermococcus profundus

Cofactor

Commentary

Organism

Structure

FAD

1.96 mol per mol of enzyme complex

Thermococcus profundus

KM Value [mM]

KM Value Maximum [mM]

Substrate

Commentary

Organism

Structure

2.5

-

L-proline

-

Thermococcus profundus

Metals/Ions

Commentary

Organism

Structure

Iron

the alpha-subunit contains the [2Fe-2S] iron-sulfur flavoprotein. The gamma subunit is a typical [8Fe-8S] ferredoxin

Thermococcus profundus

Substrates

Commentary Substrates

Literature (Substrates)

Organism

Products

Commentary (Products)

Literature (Products)

Organism (Products)

Reversibility

ID

L-proline + 2,6-dichlorophenolindophenol + H2O

-

658668

Thermococcus profundus

(S)-1-pyrroline-5-carboxylate + reduced 2,6-dichlorophenolindophenol

-

-

-

?

L-proline + ferricyanide + H2O

-

658668

Thermococcus profundus

(S)-1-pyrroline-5-carboxylate + ferrocyanide

-

-

-

?

No.

1st author

Pub Med

title

organims

journal

volume

pages

year

Activating Compound

Application

Cloned(Commentary)

Crystallization (Commentary)

Engineering

General Stability

Inhibitors

KM Value [mM]

Localization

Metals/Ions

Molecular Weight [Da]

Natural Substrates/ Products (Substrates)

Organic Solvent Stability

Organism

Oxidation Stability

Posttranslational Modification

Purification (Commentary)

Reaction

Renatured (Commentary)

Source Tissue

Specific Activity [micromol/min/mg]

Storage Stability

Substrates and Products (Substrate)

Subunits

Synonyms

Temperature Optimum [°C]

Temperature Range [°C]

Temperature Stability [°C]

Turnover Number [1/s]

pH Optimum

pH Range

pH Stability

Cofactor

Ki Value [mM]

pI Value

IC50 Value

Activating Compound (protein specific)

Application (protein specific)

Cloned(Commentary) (protein specific)

Cofactor (protein specific)

Crystallization (Commentary) (protein specific)

Engineering (protein specific)

General Stability (protein specific)

IC50 Value (protein specific)

Inhibitors (protein specific)

Ki Value [mM] (protein specific)

KM Value [mM] (protein specific)

Localization (protein specific)

Metals/Ions (protein specific)

Molecular Weight [Da] (protein specific)

Natural Substrates/ Products (Substrates) (protein specific)

Organic Solvent Stability (protein specific)

Oxidation Stability (protein specific)

Posttranslational Modification (protein specific)

Purification (Commentary) (protein specific)

Renatured (Commentary) (protein specific)

Source Tissue (protein specific)

Specific Activity [micromol/min/mg] (protein specific)

Storage Stability (protein specific)

Substrates and Products (Substrate) (protein specific)

Subunits (protein specific)

Temperature Optimum [°C] (protein specific)

Temperature Range [°C] (protein specific)

Temperature Stability [°C] (protein specific)

Turnover Number [1/s] (protein specific)

pH Optimum (protein specific)

pH Range (protein specific)

pH Stability (protein specific)

pI Value (protein specific)

Expression

General Information

General Information (protein specific)

Expression (protein specific)

KCat/KM [mM/s]

KCat/KM [mM/s] (protein specific)

743834

Huijbers

Proline dehydrogenase from Th ...

Thermus thermophilus, Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039

Sci. Rep.

7

43880

2017

-

-

1

1

1

-

-

3

-

-

-

2

-

10

-

-

-

-

-

-

4

-

4

1

1

1

-

-

3

1

-

-

3

-

-

-

-

-

1

3

1

1

-

-

-

-

3

-

-

-

2

-

-

-

-

-

-

4

-

4

1

1

-

-

3

1

-

-

-

-

-

-

-

3

3

741588

Hancock

Co-regulation of mitochondria ...

Homo sapiens, Mus musculus

Amino Acids

48

859-872

2016

-

-

2

-

1

-

12

-

2

-

-

-

-

5

-

-

1

-

-

-

-

-

-

-

4

2

-

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-

2

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-

2

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2

2

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1

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12

-

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2

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1

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-

-

2

-

-

-

2

-

-

-

-

6

6

-

-

-

741858

Cabassa-Hourton

Proteomic and functional anal ...

Arabidopsis thaliana

Biochem. J.

473

2623-2634

2016

-

-

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1

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3

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10

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1

1

1

1

-

-

741642

Wang

Molecular cloning and express ...

Jatropha curcas

Appl. Biochem. Biotechnol.

175

2413-2426

2015

-

-

1

-

-

-

-

-

-

-

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12

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6

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2

2

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1

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1

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-

-

-

-

-

-

6

-

-

-

2

-

-

-

-

-

-

-

1

1

2

2

1

-

-

742162

Huijbers

High yields of active Thermus ...

Thermus thermophilus, Thermus thermophilus HB27 / ATCC BAA-163 / DSM 7039

Biotechnol. J.

10

395-403

2015

-

1

1

-

-

-

1

-

1

-

3

2

-

8

-

-

1

-

-

-

1

-

6

1

2

1

-

2

-

1

-

-

1

-

-

-

-

1

1

1

-

-

-

-

1

-

-

1

-

3

2

-

-

-

1

-

-

1

-

6

1

1

-

2

-

1

-

-

-

-

1

1

-

-

-

740734

Moxley

Evidence for hysteretic substr ...

Escherichia coli

J. Biol. Chem.

289

3639-3651

2014

-

-

-

-

-

-

-

3

-

-

-

-

-

3

-

-

-

-

-

-

-

-

2

-

1

-

-

-

1

-

-

-

-

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-

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-

-

-

-

-

-

-

-

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-

3

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2

-

-

-

-

1

-

-

-

-

-

-

-

-

3

3

742487

Schertl

Biochemical characterization ...

Arabidopsis thaliana

FEBS J.

281

2794-2804

2014

-

-

-

-

-

-

2

-

2

-

-

1

-

6

-

-

-

-

-

-

-

-

2

1

1

-

-

-

-

-

-

-

2

-

-

-

-

-

-

2

-

-

-

-

2

-

-

2

-

-

1

-

-

-

-

-

-

-

-

2

1

-

-

-

-

-

-

-

-

1

2

2

1

-

-

724389

Serrano

Kinetic and isotopic character ...

Mycobacterium tuberculosis

Biochemistry

52

5009-5015

2013

-

-

-

-

2

-

-

3

-

-

1

-

-

4

-

-

1

-

-

-

-

-

1

1

3

-

-

-

2

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-

-

1

-

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1

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2

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3

-

-

1

-

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-

-

1

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-

-

-

1

1

-

-

-

2

-

-

-

-

-

-

-

-

2

2

741732

Kawakami

Comparative analysis of the c ...

Pyrococcus horikoshii, Thermococcus kodakarensis, Thermococcus profundus, Pyrococcus furiosus, Thermococcus kodakarensis KOD1 JCM12380, Thermococcus profundus DSM 9503, Pyrococcus horikoshii OT-3

Appl. Microbiol. Biotechnol.

97

3419-3427

2013

-

-

7

-

-

-

8

-

-

-

-

-

-

13

-

-

7

-

-

-

-

-

22

7

24

7

-

7

-

7

-

-

7

-

-

-

-

-

7

7

-

-

-

-

14

-

-

-

-

-

-

-

-

-

7

-

-

-

-

22

7

7

-

7

-

7

-

-

-

-

14

14

-

-

-

742641

Omidinia

Expression, purification and ...

Pseudomonas putida, Pseudomonas putida POS-F84

Indian J. Microbiol.

53

297-302

2013

-

-

1

-

1

-

-

-

-

-

-

-

-

8

-

-

1

-

-

-

-

-

2

2

3

1

1

-

-

1

1

-

1

-

-

-

-

-

1

1

-

1

-

-

-

-

-

-

-

-

-

-

-

-

1

-

-

-

-

2

2

1

1

-

-

1

1

-

-

-

1

1

-

-

-

743635

Paes

Proline dehydrogenase regulat ...

Trypanosoma cruzi, Trypanosoma cruzi CL Brener

PLoS ONE

8

e69419

2013

-

-

1

-

-

-

-

1

1

-

-

2

-

13

-

-

1

-

-

1

-

-

10

1

4

1

-

-

-

1

-

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2

-

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-

-

1

2

-

-

-

-

-

-

1

1

-

-

2

-

-

-

1

-

1

-

-

10

1

1

-

-

-

1

-

-

-

1

2

2

1

-

-

724345

Moxley

Rapid reaction kinetics of pro ...

Escherichia coli

Biochemistry

51

511-520

2012

-

-

-

-

-

-

-

1

-

-

-

-

-

3

-

-

-

-

-

-

-

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1

-

1

-

-

-

1

-

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-

1

-

-

-

-

-

-

1

-

-

-

-

-

-

1

-

-

-

-

-

-

-

-

-

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-

-

1

-

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1

-

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-

-

-

-

-

-

-

739932

Kawakami

L-proline dehydrogenases in hy ...

Pyrococcus horikoshii

Appl. Microbiol. Biotechnol.

93

83-93

2012

-

-

-

-

-

-

-

1

-

-

3

1

-

7

-

-

-

-

-

-

1

-

1

1

2

-

-

1

-

-

-

-

4

-

-

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4

-

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-

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1

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-

3

1

-

-

-

-

-

-

1

-

1

1

-

-

1

-

-

-

-

-

-

-

-

-

-

-

711252

Srivastava

The structure of the proline u ...

Escherichia coli

Biochemistry

49

560-569

2010

-

-

1

1

-

-

1

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-

-

-

-

-

2

-

-

-

-

-

-

-

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-

-

1

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-

-

-

-

-

-

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-

-

1

-

1

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-

1

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-

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-

696361

Ostrander

A conserved active site tyrosi ...

Escherichia coli

Biochemistry

48

951-959

2009

-

-

1

1

2

-

1

6

-

-

-

-

-

4

-

-

1

-

-

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2

-

1

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6

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1

3

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3

6

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1

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6

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671977

Zhu

Exploring the proline-dependen ...

Escherichia coli

Biochemistry

44

12297-12306

2005

-

-

-

-

4

-

-

4

1

-

-

-

-

3

-

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1

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1

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1

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4

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1

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4

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658033

Zhang

Structures of the Escherichia ...

Escherichia coli

Biochemistry

43

12539-12548

2004

-

-

1

1

1

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3

2

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1

1

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5

-

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1

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1

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2

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1

2

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1

2

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658211

Baban

Probing a hydrogen bond pair a ...

Escherichia coli

Biochim. Biophys. Acta

1701

49-59

2004

-

-

1

-

3

-

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4

-

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4

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658668

Kawakami

Gene and primary structures of ...

Thermococcus profundus

Extremophiles

8

99-108

2004

-

-

1

-

-

-

-

1

-

1

5

-

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13

-

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1

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1

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2

1

1

1

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1

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1

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1

1

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1

1

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1

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5

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659987

Lee

Structure of the proline dehyd ...

Escherichia coli

Nat. Struct. Biol.

10

109-114

2003

-

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-

1

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1

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5

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1

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1

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392565

Nadaraia

Crystallization and preliminar ...

Escherichia coli

Acta Crystallogr. Sect. D

57

1925-1927

2001

-

-

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1

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4

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1

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392570

Sakuraba

Purification, characterization ...

Thermococcus profundus

Appl. Environ. Microbiol.

67

1470-1475

2001

-

-

-

-

-

-

-

1

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3

-

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9

-

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1

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1

1

1

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2

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1

1

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1

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1

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3

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1

1

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2

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1

-

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-

-

-

-

392572

Chaudhary

-

Proline dehydrogenase activity ...

Vigna radiata

Indian J. Exp. Biol.

37

1234-1240

1999

-

-

-

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1

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1

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1

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392571

Brown

Conformational change and memb ...

Escherichia coli

J. Biol. Chem.

268

8972-8979

1993

-

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1

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1

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1

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1

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1

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-

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392569

Deutch

-

Oxidation of L-thiazolidine-4- ...

Escherichia coli

J. Gen. Microbiol.

138

1593-1598

1992

-

-

-

-

-

-

1

-

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-

-

1

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2

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1

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-

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2

-

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722591

Brown

Redesigned purification yields ...

Escherichia coli

J. Biol. Chem.

267

13086-13092

1992

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-

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2

1

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3

-

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1

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1

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1

1

2

1

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1

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1

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1

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2

1

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1

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1

-

1

1

1

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1

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392555

Wood

Membrane association of prolin ...

Escherichia coli

Proc. Natl. Acad. Sci. USA

84

373-377

1987

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1

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3

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1

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6

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392556

Graham

Proline dehydrogenase from Esc ...

Escherichia coli

J. Biol. Chem.

259

2656-2661

1984

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2

2

2

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1

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5

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2

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392560

Scarpulla

Membrane-bound proline dehydro ...

Escherichia coli

J. Biol. Chem.

253

5997-6001

1978

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5

1

1

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1

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5

4

1

1

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1

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