EC Number   |
Temperature Stability Minimum [°C] |
Temperature Stability Maximum [°C] |
Reference |
|---|
    1.1.1.37 | -999 |
- |
- |
657090 |
| 1.1.1.37 | -999 |
- |
0.4 M myo-inositol raises the Tm-value of the enzyme by 3.4°C, pinitol increases Tm by 3.8°C, glucose has no effect |
667442 |
| 1.1.1.37 | -999 |
- |
activity of purified enzyme decreases continually from 4°C to 80°C |
286660 |
| 1.1.1.37 | -10 |
35 |
at MgCl concentrations below 0.5 M or CaCl concentrations below 0.35 M, the stability of the protein increases with decreasing temperature down to -10°C. At higher concentrations (0.8-1.5 M MgCl2 or 0.5-1.0 M CaCl2), the curves are bell-shaped with symmetric decreases in stability around 4°C |
727410 |
| 1.1.1.37 | 5 |
40 |
gradual decrease in activity above 40°C |
722201 |
| 1.1.1.37 | 5 |
60 |
purified His-tagged cMDH, high stability within this range |
722201 |
| 1.1.1.37 | 10 |
45 |
the parasite enzyme activity is not affected in a broad temperature range (from 10°C to 45°C), the enzyme is also stable at 20°C and 37°C over a 30 min period |
700562 |
| 1.1.1.37 | 20 |
- |
stable for at least 30 min |
722201 |
| 1.1.1.37 | 30 |
60 |
the purified recombinant His6-tagged MDH from strain 20Z fully retains activity after 18 h exposure at 30-40°C, and has a 10% residual activity after 18 h exposure at 50°C. The enzyme loses 50% of its activity after 10 min heating at 60°C |
740975 |
| 1.1.1.37 | 30 |
70 |
the enzyme is active in a broader temperature range. It retains full activity after 18 h exposure at 30-60°C, exhibiting a 1 h half-life time at 70°C |
740975 |
