EC Number   |
Reference |
|---|
   2.3.1.B43 | - |
729708, 730245, 730347, 730406, 730706, 730974 |
| 2.3.1.B43 | crystals are grown by hanging drop vapor diffusion method, truncated SIRT5(34302) is cloned and expressed in Escherichia coli |
729993 |
| 2.3.1.B43 | expressed in Escherichia coli BL21(DE3) cells |
739445 |
| 2.3.1.B43 | expression in Escherichia coli |
730713 |
| 2.3.1.B43 | gene SIRT5 |
758247 |
| 2.3.1.B43 | gene SIRT5, alternative splicing of the human SIRT5 mRNA results in two distinct SIRT5 protein isoforms, differing at their C-termini, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3, method, overview) |
757590 |
| 2.3.1.B43 | gene SIRT5, genotyping of the Ctl and OE mice. Sirt5 hepatic overexpressing (ob/ob-SIRT5 OE) mouse containing at least one allele of alternative Sirt5 gene and Alb-cre. The other one is control ob/ob mouse (ob/ob- control) containing only the floxed Sirt5 KI allele |
756563 |
| 2.3.1.B43 | gene SIRT5, quantitative real-time PCR expression analysis |
756555 |
| 2.3.1.B43 | gene SIRT5, recombinant expression of His-tagged enzyme in Escherichia coli strains Rosetta2(DE3) and BL21(DE3) |
756010 |
| 2.3.1.B43 | gene SIRT5, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) |
757400 |
