EC Number   |
|---|
  2.1.1.182 | - |
| 2.1.1.182 | cloning of ksgA from the 431DELTA99 KSGR mutant |
| 2.1.1.182 | expression of a pGEX-KsgA fusion construct in Escherichia coli KSR7 |
| 2.1.1.182 | expression of the Chlamydia trachomatis L2 KsgA ortholog restored kasugamycin sensitivity to the Escherichia coli ksgA mutant |
| 2.1.1.182 | expression of wild-type and mutant enzymes in Escherichia coli strain XL1-Blue |
| 2.1.1.182 | full-length ksgA gene is cloned into the expression vector pET26b and overexpressed in Escherichia coli strain BL21Star |
| 2.1.1.182 | KsgA engineered with an N-terminal polyhistidine tag is overexpressed in Escherichia coli cells |
