EC Number   |
|---|
    1.3.99.4 | - |
| 1.3.99.4 | expressed in Bacillus subtilis strain WB600 |
| 1.3.99.4 | expressed in Escherichia coli BL21(DE3) cells |
| 1.3.99.4 | expression in Escherichia coli |
| 1.3.99.4 | expression in Streptomyces lividans |
| 1.3.99.4 | gene acmB, a synthetic DELTA1-KstD, developed based on the AcmB gene of Sterolibacterium denitrificans, stable recombinant expression in Escherichia coli and in human Hep-3B and U-937 cells. The enzyme, when expressed in human cells, introduces a double bond between the C-1 and C-2 atoms of 3-oxosteroids. This represents an integral step needed for cholestane A-ring aromatization and B-ring opening for which humans have no known orthologue, coexpression with cholesterol-3-hydroxy-dehydrogenase (CholD) and 3-oxosteroid-9alpha-hydroxylase (Kst-9alphaH) |
| 1.3.99.4 | gene acmB, recombinant expression in Escherichia coli |
| 1.3.99.4 | gene amcB, recombinant enzyme expression in Escherichia coli |
| 1.3.99.4 | gene ksdD, DNA and amino acid sequence determination and analysis, phylogenetic analysis and tree, recombinant overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) |
| 1.3.99.4 | gene ksdD, phylogenetic analysis, expression in Bacillus subtilis strain 168 with 18fold higher activity compared to wild-type Mycobacterium neoaurum strain JC-12 |
