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Information on EC 1.3.99.4 - 3-oxosteroid 1-dehydrogenase
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1.3.99.4 3-oxosteroid 1-dehydrogenaseSpecify your search results
Word Map
- 1.3.99.4
- rhodococcus
- mycobacterium
- 3-ketosteroids
- cholesterol
- 4-androstene-3,17-dione
- erythropolis
- isoenzymes
- testosteroni
-
arthrobacter
- synthesis
- simplex
- sterol
- androst-4-ene-3,17-dione
- rhodochrous
- globiformis
- phytosterols
-
steroid-transforming
- 1,4-androstadiene-3,17-dione
- hydrocortisone
-
bioconversion
-
dehydrogenation
- neoaurum
- ruber
The expected taxonomic range for this enzyme is: Bacteria, Eukaryota, Archaea
Synonyms
1-ene-dehydrogenase, 3-keto-5alpha-steroid DELTA(1)-dehydrogenase, 3-ketosteroid 1(2)-dehydrogenase, 3-ketosteroid delta1-dehydrogenase, 3-ketosteroid-1-en-dehydrogenase, 3-ketosteroid-D1-dehydrogenase, 3-ketosteroid-DELTA1-dehydrogenase, 3-oxosteroid D1-dehydrogenase, 3-oxosteroid Delta1-dehydrogenase, 3-oxosteroid:(2,6-dichlorphenolindophenol) DELTA1-oxidoreductase, 
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
1-ene-dehydrogenase
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-
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3-keto-5alpha-steroid DELTA(1)-dehydrogenase
3-ketosteroid 1(2)-dehydrogenase
3-ketosteroid delta1-dehydrogenase
3-ketosteroid-1-en-dehydrogenase
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3-ketosteroid-D1-dehydrogenase
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3-ketosteroid-DELTA1-dehydrogenase
3-oxosteroid D1-dehydrogenase
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3-oxosteroid Delta1-dehydrogenase
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3-oxosteroid:(2,6-dichlorphenolindophenol) DELTA1-oxidoreductase
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3-oxosteroid:(2,6-dichlorphenolindophenol)D1-oxidoreductase
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4-en-3-oxosteroid:(acceptor)-1-en-oxido-reductase
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4-ene-3-oxosteroid: (acceptor)-1-ene-oxoreductase
4-ene-3-oxosteroid:(acceptor)l-ene-oxidoreductase
D1-dehydrogenase
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D1-steroid reductase
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dehydrogenase, 3-oxo steroid Delta 1-
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Delta1-dehydrogenase
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DELTA1-KSTD1
DELTA1-steroid reductase
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ketosteroid DELTA1-dehydrogenase
KsdD
KsdD1
KsdD2
KsdDM
KstD
KSTD1
KSTD2
KSTD3
KSTD3 orthologue
KstDF
Rv3537
steroid-1-dehydrogenase
3-keto-5alpha-steroid DELTA(1)-dehydrogenase
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3-ketosteroid-DELTA1-dehydrogenase
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4-ene-3-oxosteroid: (acceptor)-1-ene-oxoreductase
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4-ene-3-oxosteroid: (acceptor)-1-ene-oxoreductase
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-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
a 3-oxosteroid + acceptor = a 3-oxo-DELTA1-steroid + reduced acceptor
stereochemistry: trans-diaxial
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a 3-oxosteroid + acceptor = a 3-oxo-DELTA1-steroid + reduced acceptor
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
oxidation
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redox reaction
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reduction
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PATHWAY SOURCE
PATHWAYS
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SYSTEMATIC NAME
IUBMB Comments
3-oxosteroid:acceptor DELTA1-oxidoreductase
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CAS REGISTRY NUMBER
COMMENTARY
9029-04-3
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(5alpha,17beta)-17-hydroxyandrostan-3-one + 2,6-dichlorophenol-indophenol
? + reduced 2,6-dichlorophenol-indophenol
11-deoxy-17alpha-hydroxycorticosterone + acceptor
17,21-dihydroxypregna-1,4-diene-3,20-dione + reduced acceptor
-
4-pregnene-17alpha,21-diol-3,20-dione
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-
?
11alpha-hydroxy-testosterone + acceptor
11alpha,17beta-dihydroxyandrost-1,4-diene-3-one + reduced acceptor
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-
-
-
?
11alpha-hydroxyprogesterone + acceptor
11alpha-hydroxy-1,4-pregnene-3,20-dione + reduced acceptor
-
4-pregnene-11alpha-ol-3,20-dione
-
-
?
11beta-cortisol + 2,6-dichlorophenol-indophenol
(11beta)-11,17,21-trihydroxypregna-1,4-diene-3,20-dione + reduced 2,6-dichlorophenol-indophenol
11beta-hydroxy-4-androstene-3,17-dione + acceptor
11beta-hydroxy-1,4-androstene-3,17-dione + reduced acceptor
11beta-hydroxytestosterone + acceptor
11beta,17beta-dihydroxyandrost-1,4-diene-3-one + reduced acceptor
-
4-androstene-11beta,17beta-diol-3-one
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-
?
17alpha-hydroxy-progesterone + acceptor
1,4-pregnene-17alpha-ol-3,20-dione + reduced acceptor
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4-pregnene-17alpha-ol-3,20-dione
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-
?
17alpha-methyltestosterone + acceptor
17alpha-methylandrost-1,4-diene-3-one + reduced acceptor
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-
-
-
?
19-nor-4-androstene-3,17-dione + acceptor
19-nor-1,4-androstene-3,17-dione + reduced acceptor
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4-estren-3,17-dione
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-
?
19-nortestosterone + acceptor
17beta-hydroxy-1,4-estrene-3-one + reduced acceptor
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4-estren-17beta-ol-3-one
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-
?
19alpha-hydroxy-4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
19alpha-hydroxy-androst-1,4-diene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
3-oxo-5beta-cholan-24-oic acid + acceptor
? + reduced acceptor
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-
-
-
?
3-oxosteroid + acceptor
3-oxo-DELTA1-steroid + reduced acceptor
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key catalyst in microbial steroid metabolism
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-
?
4-androstene-17beta-ol-3-one + 2,6-dichlorophenol-indophenol
1,4-androstadiene-17beta-ol-3-one + reduced 2,6-dichlorophenol-indophenol
4-androstene-3,11,17-trione + acceptor
1,4-androstene-3,11,17-trione + reduced acceptor
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-
-
-
?
4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
1,4-androstadiene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
1,4-androstene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
4-androstene-3,17-dione + phenazine methosulfate
1,4-androstadiene-3,17-dione + reduced phenazine methosulfate
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r
4-androstene-3,17-dione + phenazine methosulfate
1,4-androstene-3,17-dione + reduced phenazine methosulfate
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-
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?
4-pregnen-3-one-20beta-carboxylic acid + 2,6-dichlorophenol-indophenol
pregna-1,4-dien-3-one-20beta-carboxylic acid + reduced 2,6-dichlorophenol-indophenol
5alpha-androst-1-en-3-one + acceptor
5alpha-androstan-3-one + reduced acceptor
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-
-
-
?
5alpha-androstan-3-one-17beta-ol + 2,6-dichlorophenol-indophenol
(5alpha,17beta)-17-hydroxyandrost-1-en-3-one + reduced 2,6-dichlorophenol-indophenol
5alpha-androstane-17beta-ol-3-one + 2,6-dichlorophenol-indophenol
5alpha-androst-1-ene-17beta-ol-3-one + reduced 2,6-dichlorophenol-indophenol
5alpha-androstane-3,17-dione + 2,6-dichlorophenol-indophenol
5beta-androst-1-en-3,17-dione + reduced 2,6-dichlorophenol-indophenol
5alpha-pregnane-3,20-dione + 2,6-dichlorophenol-indophenol
5alpha-pregn-1-en-3,20-dione + reduced 2,6-dichlorophenol-indophenol
5beta-androstan-3,17-dione + acceptor
5beta-androst-1-en-3,17-dione + reduced acceptor
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?
9alpha-hydroxy-4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
9alpha-hydroxy-1,4-androstadiene 3,17-dione + reduced 2,6-dichlorophenol-indophenol
9alpha-hydroxy-4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
9alpha-hydroxy-androst-1,4-diene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
9alpha-hydroxy-4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
9alpha-hydroxyandrost-1,4-diene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
9alpha-hydroxy-4-androstene-3,17-dione + acceptor
9alpha-hydroxy-1,4-androstadiene 3,17-dione + reduced acceptor
9alpha-hydroxy-4-androstene-3,17-dione + FAD
9alpha-hydroxy-1,4-androstadiene-3,17-dione + FADH2
androsterone + acceptor
3-alpha-hydroxy-1-androstene-17-one + reduced acceptor
-
4-androstene-3,11,17-trione
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-
?
corticosterone + 2,6-dichlorophenol-indophenol
1,4-pregnene-17alpha,21-diol-3,20-dione + reduced 2,6-dichlorophenol-indophenol
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-
-
?
corticosterone + acceptor
1,4-pregnene-17alpha,21-diol-3,20-dione + reduced acceptor
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4-pregnene-11beta,21-diol-3,20-dione
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?
cortisol + acceptor
prednisolone + reduced acceptor
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optimal concentration is 0.1 mg/ml, increase of concentration to 1 mg/ml reduces prednisolone yield
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?
deoxycorticosterone + 2,6-dichlorophenol-indophenol
1,4-pregnadiene-21-ol-3,20-dione + reduced 2,6-dichlorophenol-indophenol
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-
-
?
deoxycorticosterone + 2,6-dichlorophenol-indophenol
1,4-pregnene-21-ol-3,20-dione + reduced 2,6-dichlorophenol-indophenol
deoxycorticosterone + acceptor
1,4-pregnene-21-ol-3,20-dione + reduced acceptor
-
4-pregnene-21-ol-3,20-dione
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-
?
hydrocorticosterone + acceptor
1,4-pregnene-11beta,17alpha,21-triol-3,20-dione + reduced acceptor
-
4-pregnene-11beta,17alpha,21triol-3,20-dione
-
-
?
progesterone + 2,6-dichlorophenol-indophenol
pregna-1,4-diene + reduced 2,6-dichlorophenol-indophenol
progesterone + 2,6-dichlorophenol-indophenol
pregna-1,4-diene-3,20-dione + reduced 2,6-dichlorophenol-indophenol
testololactone + acceptor
D-homo-17alpha-oxoandrosta-1,4-diene-3,17-one + reduced acceptor
-
D-homo-17a-oxoandrost-4-ene-3,17-dione
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-
?
testosterone + 2,6-dichlorophenol-indophenol
17beta-hydroxyandrost-1,4-diene-3-one + reduced 2,6-dichlorophenol-indophenol
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-
-
?
(5alpha,17beta)-17-hydroxyandrostan-3-one + 2,6-dichlorophenol-indophenol
? + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
(5alpha,17beta)-17-hydroxyandrostan-3-one + 2,6-dichlorophenol-indophenol
? + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
11beta-cortisol + 2,6-dichlorophenol-indophenol
(11beta)-11,17,21-trihydroxypregna-1,4-diene-3,20-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
11beta-cortisol + 2,6-dichlorophenol-indophenol
(11beta)-11,17,21-trihydroxypregna-1,4-diene-3,20-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
11beta-hydroxy-4-androstene-3,17-dione + acceptor
11beta-hydroxy-1,4-androstene-3,17-dione + reduced acceptor
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-
-
-
?
11beta-hydroxy-4-androstene-3,17-dione + acceptor
11beta-hydroxy-1,4-androstene-3,17-dione + reduced acceptor
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-
-
-
?
19alpha-hydroxy-4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
19alpha-hydroxy-androst-1,4-diene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
19alpha-hydroxy-4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
19alpha-hydroxy-androst-1,4-diene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
23,24-bis-nor-5alpha-cholestan-3-one acid + 2,6-dichlorophenol-indophenol
?
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-
?
23,24-bis-nor-5alpha-cholestan-3-one acid + 2,6-dichlorophenol-indophenol
?
-
-
-
?
4-androstene-17beta-ol-3-one + 2,6-dichlorophenol-indophenol
1,4-androstadiene-17beta-ol-3-one + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
4-androstene-17beta-ol-3-one + 2,6-dichlorophenol-indophenol
1,4-androstadiene-17beta-ol-3-one + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
1,4-androstadiene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
-
?
4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
1,4-androstadiene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
1,4-androstadiene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
1,4-androstadiene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
-
?
4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
1,4-androstadiene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
Mycolicibacterium neoaurum ST-095
-
-
-
-
?
4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
1,4-androstadiene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
1,4-androstadiene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
1,4-androstadiene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
1,4-androstadiene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
1,4-androstene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
1,4-androstene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
4-androstene-3,17-dione + acceptor
1,4-androstadiene-3,17-dione + reduced acceptor
involved in steroid catabolism
-
-
?
4-androstene-3,17-dione + acceptor
1,4-androstadiene-3,17-dione + reduced acceptor
acceptor: phenazine methosulfate/2,6-dichlorophenolindophenol system. The enzyme does not show any activity in presence of nitro blue tetrazolium
-
-
?
4-androstene-3,17-dione + acceptor
1,4-androstadiene-3,17-dione + reduced acceptor
involved in steroid catabolism
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-
?
4-androstene-3,17-dione + acceptor
1,4-androstadiene-3,17-dione + reduced acceptor
acceptor: phenazine methosulfate/2,6-dichlorophenolindophenol system. The enzyme does not show any activity in presence of nitro blue tetrazolium
-
-
?
4-androstene-3,17-dione + acceptor
1,4-androstene-3,17-dione + reduced acceptor
-
-
-
-
ir
4-androstene-3,17-dione + acceptor
1,4-androstene-3,17-dione + reduced acceptor
-
-
-
-
?
4-androstene-3,17-dione + acceptor
1,4-androstene-3,17-dione + reduced acceptor
-
-
-
-
?
4-androstene-3,17-dione + acceptor
1,4-androstene-3,17-dione + reduced acceptor
-
-
-
-
?
4-androstene-3,17-dione + acceptor
1,4-androstene-3,17-dione + reduced acceptor
-
-
-
-
?
4-androstene-3,17-dione + acceptor
1,4-androstene-3,17-dione + reduced acceptor
-
-
-
-
?
4-androstene-3,17-dione + acceptor
1,4-androstene-3,17-dione + reduced acceptor
-
-
-
-
r
4-androstene-3,17-dione + FAD
1,4-androstadiene-3,17-dione + FADH2
-
-
-
-
r
4-androstene-3,17-dione + FAD
1,4-androstadiene-3,17-dione + FADH2
-
-
-
-
r
4-androstene-3,17-dione + FAD
1,4-androstadiene-3,17-dione + FADH2
-
-
-
?
4-androstene-3,17-dione + FAD
1,4-androstadiene-3,17-dione + FADH2
-
-
-
-
?
4-androstene-3,17-dione + FAD
1,4-androstadiene-3,17-dione + FADH2
with artificial electron acceptors phenazine methosulfate and dichlorophenolindophenol
-
-
?
4-androstene-3,17-dione + FAD
1,4-androstadiene-3,17-dione + FADH2
-
with artificial electron acceptors phenazine methosulfate and dichlorophenolindophenol
-
-
?
4-androstene-3,17-dione + FAD
1,4-androstadiene-3,17-dione + FADH2
-
-
-
-
?
4-androstene-3,17-dione + FAD
1,4-androstadiene-3,17-dione + FADH2
-
with artificial electron acceptors phenazine methosulfate and dichlorophenolindophenol
-
-
?
4-androstene-3,17-dione + FAD
1,4-androstadiene-3,17-dione + FADH2
Mycolicibacterium neoaurum NwIB-01
-
-
-
?
4-androstene-3,17-dione + FAD
1,4-androstadiene-3,17-dione + FADH2
Mycolicibacterium neoaurum NwIB-01
with artificial electron acceptors phenazine methosulfate and dichlorophenolindophenol
-
-
?
4-androstene-3,17-dione + FAD
1,4-androstadiene-3,17-dione + FADH2
-
-
-
?
4-androstene-3,17-dione + FAD
1,4-androstadiene-3,17-dione + FADH2
-
-
-
?
4-pregnen-3-one-20beta-carboxylic acid + 2,6-dichlorophenol-indophenol
pregna-1,4-dien-3-one-20beta-carboxylic acid + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
4-pregnen-3-one-20beta-carboxylic acid + 2,6-dichlorophenol-indophenol
pregna-1,4-dien-3-one-20beta-carboxylic acid + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
4-pregnene-3,20-dione + acceptor
1,4-pregnene-3,20 dione + reduced acceptor
-
-
-
-
?
4-pregnene-3,20-dione + acceptor
1,4-pregnene-3,20 dione + reduced acceptor
-
-
-
-
?
4-pregnene-3,20-dione + acceptor
1,4-pregnene-3,20 dione + reduced acceptor
-
-
-
?
4-pregnene-3,20-dione + acceptor
1,4-pregnene-3,20 dione + reduced acceptor
-
-
-
?
5alpha-androstan-3-one-17beta-ol + 2,6-dichlorophenol-indophenol
(5alpha,17beta)-17-hydroxyandrost-1-en-3-one + reduced 2,6-dichlorophenol-indophenol
-
KSTD3 shows highest activity on 5alpha-testosterone which is also known as 17beta-hydroxy-5alpha-androstane-3-one
-
-
?
5alpha-androstan-3-one-17beta-ol + 2,6-dichlorophenol-indophenol
(5alpha,17beta)-17-hydroxyandrost-1-en-3-one + reduced 2,6-dichlorophenol-indophenol
-
KSTD3 shows highest activity on 5alpha-testosterone which is also known as 17beta-hydroxy-5alpha-androstane-3-one
-
-
?
5alpha-androstan-3-one-17beta-ol + 2,6-dichlorophenol-indophenol
(5alpha,17beta)-17-hydroxyandrost-1-en-3-one + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
5alpha-androstan-3-one-17beta-ol + 2,6-dichlorophenol-indophenol
(5alpha,17beta)-17-hydroxyandrost-1-en-3-one + reduced 2,6-dichlorophenol-indophenol
KSTD3 shows highest activity on 5alpha-androstan-3-one-17beta-ol which is also known as 17beta-hydroxy-5alpha-androstane-3-one
-
-
?
5alpha-androstan-3-one-17beta-ol + 2,6-dichlorophenol-indophenol
(5alpha,17beta)-17-hydroxyandrost-1-en-3-one + reduced 2,6-dichlorophenol-indophenol
KSTD3 shows highest activity on 5alpha-androstan-3-one-17beta-ol which is also known as 17beta-hydroxy-5alpha-androstane-3-one
-
-
?
5alpha-androstan-3-one-17beta-ol + 2,6-dichlorophenol-indophenol
(5alpha,17beta)-17-hydroxyandrost-1-en-3-one + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
5alpha-androstane-17beta-ol-3-one + 2,6-dichlorophenol-indophenol
5alpha-androst-1-ene-17beta-ol-3-one + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
5alpha-androstane-17beta-ol-3-one + 2,6-dichlorophenol-indophenol
5alpha-androst-1-ene-17beta-ol-3-one + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
5alpha-androstane-3,17-dione + 2,6-dichlorophenol-indophenol
5beta-androst-1-en-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
KSTD3 shows highest activity with 5alpha-androstane-3,17-dione
-
-
?
5alpha-androstane-3,17-dione + 2,6-dichlorophenol-indophenol
5beta-androst-1-en-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
KSTD3 shows highest activity with 5alpha-androstane-3,17-dione
-
-
?
5alpha-androstane-3,17-dione + 2,6-dichlorophenol-indophenol
5beta-androst-1-en-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
5alpha-androstane-3,17-dione + 2,6-dichlorophenol-indophenol
5beta-androst-1-en-3,17-dione + reduced 2,6-dichlorophenol-indophenol
KSTD3 shows highest activity with 5alpha-androstane-3,17-dione
-
-
?
5alpha-androstane-3,17-dione + 2,6-dichlorophenol-indophenol
5beta-androst-1-en-3,17-dione + reduced 2,6-dichlorophenol-indophenol
KSTD3 shows highest activity with 5alpha-androstane-3,17-dione
-
-
?
5alpha-pregnane-3,20-dione + 2,6-dichlorophenol-indophenol
5alpha-pregn-1-en-3,20-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
5alpha-pregnane-3,20-dione + 2,6-dichlorophenol-indophenol
5alpha-pregn-1-en-3,20-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
9alpha-hydroxy-4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
9alpha-hydroxy-1,4-androstadiene 3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
9alpha-hydroxy-4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
9alpha-hydroxy-1,4-androstadiene 3,17-dione + reduced 2,6-dichlorophenol-indophenol
preferred substrate of isozyme KSTD2
-
-
?
9alpha-hydroxy-4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
9alpha-hydroxy-androst-1,4-diene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
9alpha-hydroxy-4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
9alpha-hydroxy-androst-1,4-diene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
9alpha-hydroxy-4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
9alpha-hydroxy-androst-1,4-diene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
9alpha-hydroxy-4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
9alpha-hydroxyandrost-1,4-diene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
9alpha-hydroxy-4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
9alpha-hydroxyandrost-1,4-diene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
9alpha-hydroxy-4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
9alpha-hydroxyandrost-1,4-diene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
9alpha-hydroxy-4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
9alpha-hydroxyandrost-1,4-diene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
9alpha-hydroxy-4-androstene-3,17-dione + acceptor
9alpha-hydroxy-1,4-androstadiene 3,17-dione + reduced acceptor
involved in steroid catabolism
-
-
?
9alpha-hydroxy-4-androstene-3,17-dione + acceptor
9alpha-hydroxy-1,4-androstadiene 3,17-dione + reduced acceptor
acceptor: phenazine methosulfate/2,6-dichlorophenolindophenol system. The enzyme does not show any activity in presence of nitro blue tetrazolium
-
-
?
9alpha-hydroxy-4-androstene-3,17-dione + acceptor
9alpha-hydroxy-1,4-androstadiene 3,17-dione + reduced acceptor
involved in steroid catabolism
-
-
?
9alpha-hydroxy-4-androstene-3,17-dione + acceptor
9alpha-hydroxy-1,4-androstadiene 3,17-dione + reduced acceptor
acceptor: phenazine methosulfate/2,6-dichlorophenolindophenol system. The enzyme does not show any activity in presence of nitro blue tetrazolium
-
-
?
9alpha-hydroxy-4-androstene-3,17-dione + FAD
9alpha-hydroxy-1,4-androstadiene-3,17-dione + FADH2
-
-
-
?
9alpha-hydroxy-4-androstene-3,17-dione + FAD
9alpha-hydroxy-1,4-androstadiene-3,17-dione + FADH2
Mycolicibacterium neoaurum NwIB-01
-
-
-
?
cholesterol + acceptor
?
-
KsdD2 exhibits low but detectable activity in the cholesterol degradation process
-
-
?
cholesterol + acceptor
?
Mycolicibacterium smegmatis mc(2)155 / ATCC 700084
-
KsdD2 exhibits low but detectable activity in the cholesterol degradation process
-
-
?
cortisone + acceptor
1,4-pregnene-17alpha,21-diol-3,11,20-trione + reduced acceptor
-
4-pregnene-17alpha,21-diol-3,11,20-trione
-
-
?
cortisone + acceptor
1,4-pregnene-17alpha,21-diol-3,11,20-trione + reduced acceptor
-
-
-
-
?
deoxycorticosterone + 2,6-dichlorophenol-indophenol
1,4-pregnene-21-ol-3,20-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
deoxycorticosterone + 2,6-dichlorophenol-indophenol
1,4-pregnene-21-ol-3,20-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
progesterone + 2,6-dichlorophenol-indophenol
pregna-1,4-diene + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
progesterone + 2,6-dichlorophenol-indophenol
pregna-1,4-diene + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
progesterone + 2,6-dichlorophenol-indophenol
pregna-1,4-diene-3,20-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
-
?
progesterone + 2,6-dichlorophenol-indophenol
pregna-1,4-diene-3,20-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
-
?
progesterone + 2,6-dichlorophenol-indophenol
pregna-1,4-diene-3,20-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
progesterone + 2,6-dichlorophenol-indophenol
pregna-1,4-diene-3,20-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
progesterone + 2,6-dichlorophenol-indophenol
pregna-1,4-diene-3,20-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
progesterone + 2,6-dichlorophenol-indophenol
pregna-1,4-diene-3,20-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
progesterone + 2,6-dichlorophenol-indophenol
pregna-1,4-diene-3,20-dione + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
testosterone + 2,6-dichlorophenol-indophenol
? + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
testosterone + 2,6-dichlorophenol-indophenol
? + reduced 2,6-dichlorophenol-indophenol
-
-
-
?
testosterone + acceptor
17beta-hydroxyandrost-1,4-diene-3-one + reduced acceptor
-
-
-
-
?
testosterone + acceptor
17beta-hydroxyandrost-1,4-diene-3-one + reduced acceptor
-
-
-
-
?
additional information
?
-
-
acceptor: phenazine methosulfate
-
-
?
additional information
?
-
-
3-oxo-5beta-steroid DELTA1-dehydrogenase is active only on 5beta-steroids
-
-
?
additional information
?
-
enzyme works in an early step of steroid degradation
-
-
?
additional information
?
-
enzyme works in an early step of steroid degradation
-
-
?
additional information
?
-
-
not: 3beta-hydroxysteroid, no electron acceptor: NADP+, K3Fe(CN)6, cytochrome c, 3-ketosteroids with a 11alpha- or 11beta-hydroxyl group are oxidized at slow rate, catalyzes aromatization of the A-ring of 19-nortestosterone and 19-norandrostenedione to produce estradiol and estrone, 19-hydroxytestosterone, 19-hydroxyandrosterone, 19-hydroxyandrostenedione and 19-oxotestosterone are converted to the respective phenolic steroids with cleavage of the C10 side-chain
-
-
?
additional information
?
-
-
acceptor: vitamin K2 (35), only 1-4% product formation as compared to 85-98% formation of 1,4-androstadiene-3,17-dione when phenazine methosulfate or menadione are used
-
-
?
additional information
?
-
-
4-androstene-3,17-dione, 9alpha-hydroxy-4-androstene-3,17-dione, 11beta-cortisol, 23,24-bisnor-5alpha-cholestan-3-one acid, 5beta-androstane-3,17-dione, [1-(5alpha)-androstene-3,17-dione], 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 5alpha-pregnane-3,20-dione, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol, 17-one are no substrates
-
-
?
additional information
?
-
-
4-androstene-3,17-dione, 9alpha-hydroxy-4-androstene-3,17-dione, 11beta-cortisol, 23,24-bisnor-5alpha-cholestan-3-one acid, 5beta-androstane-3,17-dione, [1-(5alpha)-androstene-3,17-dione], 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 5alpha-pregnane-3,20-dione, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol, 17-one are no substrates
-
-
?
additional information
?
-
-
substrate must have a 3-oxo-group, a DELTA4-double bond and axial hydrogens at 1alpha and 2beta positions
-
-
?
additional information
?
-
-
acceptor: phenazine methosulfate
-
-
?
additional information
?
-
-
acceptor: vitamin K2 (35), only 1-4% product formation as compared to 85-98% formation of 1,4-androstadiene-3,17-dione when phenazine methosulfate or menadione are used
-
-
?
additional information
?
-
4-androstene-3,17-dione, 9alpha-hydroxy-4-androstene-3,17-dione, 11beta-cortisol, 23,24-bis-nor-5alpha-cholestan-3-one acid, 5beta-androstane-3,17-dione, 1-(5alpha)-androstene-3,17-dione, 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol,17-one are no substrates
-
-
?
additional information
?
-
4-androstene-3,17-dione, 9alpha-hydroxy-4-androstene-3,17-dione, 11beta-cortisol, 23,24-bis-nor-5alpha-cholestan-3-one acid, 5beta-androstane-3,17-dione, 1-(5alpha)-androstene-3,17-dione, 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol,17-one are no substrates
-
-
?
additional information
?
-
4-androstene-3,17-dione, 9alpha-hydroxy-4-androstene-3,17-dione, 11beta-cortisol, 23,24-bis-nor-5alpha-cholestan-3-one acid, 5beta-androstane-3,17-dione, 1-(5alpha)-androstene-3,17-dione, 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol,17-one are no substrates
-
-
?
additional information
?
-
-
4-androstene-3,17-dione, 9alpha-hydroxy-4-androstene-3,17-dione, 11beta-cortisol, 23,24-bis-nor-5alpha-cholestan-3-one acid, 5beta-androstane-3,17-dione, 1-(5alpha)-androstene-3,17-dione, 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol,17-one are no substrates
-
-
?
additional information
?
-
5beta-androstane-3,17-dione, 1-(5alpha)-androstene-3,17-dione, 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol,17-one are no substrates
-
-
?
additional information
?
-
5beta-androstane-3,17-dione, 1-(5alpha)-androstene-3,17-dione, 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol,17-one are no substrates
-
-
?
additional information
?
-
5beta-androstane-3,17-dione, 1-(5alpha)-androstene-3,17-dione, 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol,17-one are no substrates
-
-
?
additional information
?
-
-
5beta-androstane-3,17-dione, 1-(5alpha)-androstene-3,17-dione, 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol,17-one are no substrates
-
-
?
additional information
?
-
5beta-androstane-3,17-dione, [1-(5alpha)-androstene-3,17-dione], 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol,17-one are no substrates
-
-
?
additional information
?
-
5beta-androstane-3,17-dione, [1-(5alpha)-androstene-3,17-dione], 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol,17-one are no substrates
-
-
?
additional information
?
-
5beta-androstane-3,17-dione, [1-(5alpha)-androstene-3,17-dione], 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol,17-one are no substrates
-
-
?
additional information
?
-
-
5beta-androstane-3,17-dione, [1-(5alpha)-androstene-3,17-dione], 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol,17-one are no substrates
-
-
?
additional information
?
-
in catalytic mechanism Tyr487 and Gly491 work in tandem to promote keto-enol tautomerization and increase the acidity of the C2 hydrogen atoms of the substrate. With assistance of Tyr119, the general base Tyr318 abstracts the axialbeta-hydrogen from C2 as a proton, whereas the FAD accepts the axial alpha-hydrogen from the C1 atom of the substrate as a hydride ion
-
-
?
additional information
?
-
4-androstene-3,17-dione, 9alpha-hydroxy-4-androstene-3,17-dione, 11beta-cortisol, 23,24-bis-nor-5alpha-cholestan-3-one acid, 5beta-androstane-3,17-dione, 1-(5alpha)-androstene-3,17-dione, 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol,17-one are no substrates
-
-
?
additional information
?
-
4-androstene-3,17-dione, 9alpha-hydroxy-4-androstene-3,17-dione, 11beta-cortisol, 23,24-bis-nor-5alpha-cholestan-3-one acid, 5beta-androstane-3,17-dione, 1-(5alpha)-androstene-3,17-dione, 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol,17-one are no substrates
-
-
?
additional information
?
-
4-androstene-3,17-dione, 9alpha-hydroxy-4-androstene-3,17-dione, 11beta-cortisol, 23,24-bis-nor-5alpha-cholestan-3-one acid, 5beta-androstane-3,17-dione, 1-(5alpha)-androstene-3,17-dione, 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol,17-one are no substrates
-
-
?
additional information
?
-
-
4-androstene-3,17-dione, 9alpha-hydroxy-4-androstene-3,17-dione, 11beta-cortisol, 23,24-bis-nor-5alpha-cholestan-3-one acid, 5beta-androstane-3,17-dione, 1-(5alpha)-androstene-3,17-dione, 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol,17-one are no substrates
-
-
?
additional information
?
-
5beta-androstane-3,17-dione, [1-(5alpha)-androstene-3,17-dione], 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol,17-one are no substrates
-
-
?
additional information
?
-
5beta-androstane-3,17-dione, [1-(5alpha)-androstene-3,17-dione], 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol,17-one are no substrates
-
-
?
additional information
?
-
5beta-androstane-3,17-dione, [1-(5alpha)-androstene-3,17-dione], 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol,17-one are no substrates
-
-
?
additional information
?
-
-
5beta-androstane-3,17-dione, [1-(5alpha)-androstene-3,17-dione], 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol,17-one are no substrates
-
-
?
additional information
?
-
in catalytic mechanism Tyr487 and Gly491 work in tandem to promote keto-enol tautomerization and increase the acidity of the C2 hydrogen atoms of the substrate. With assistance of Tyr119, the general base Tyr318 abstracts the axialbeta-hydrogen from C2 as a proton, whereas the FAD accepts the axial alpha-hydrogen from the C1 atom of the substrate as a hydride ion
-
-
?
additional information
?
-
5beta-androstane-3,17-dione, 1-(5alpha)-androstene-3,17-dione, 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol,17-one are no substrates
-
-
?
additional information
?
-
5beta-androstane-3,17-dione, 1-(5alpha)-androstene-3,17-dione, 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol,17-one are no substrates
-
-
?
additional information
?
-
5beta-androstane-3,17-dione, 1-(5alpha)-androstene-3,17-dione, 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol,17-one are no substrates
-
-
?
additional information
?
-
-
5beta-androstane-3,17-dione, 1-(5alpha)-androstene-3,17-dione, 11alpha-hydroxy-4-norandrostene-3,17-dione, 1,4-androstadiene-3,17-dione, dehydroisoandrosterone, 4-cholesten-3-one, 5alpha-cholestan-3-one, and 5alpha-androstan-3beta-ol,17-one are no substrates
-
-
?
additional information
?
-
-
acceptor: vitamin K2 (35), only 1-4% product formation as compared to 85-98% formation of 1,4-androstadiene-3,17-dione when phenazine methosulfate or menadione are used
-
-
?
Please wait a moment until the data is sorted. This message will disappear when the data is sorted.
NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
3-oxosteroid + acceptor
3-oxo-DELTA1-steroid + reduced acceptor
-
key catalyst in microbial steroid metabolism
-
-
?
9alpha-hydroxy-4-androstene-3,17-dione + acceptor
9alpha-hydroxy-1,4-androstadiene 3,17-dione + reduced acceptor
4-androstene-3,17-dione + acceptor
1,4-androstadiene-3,17-dione + reduced acceptor
involved in steroid catabolism
-
-
?
4-androstene-3,17-dione + acceptor
1,4-androstadiene-3,17-dione + reduced acceptor
involved in steroid catabolism
-
-
?
4-androstene-3,17-dione + FAD
1,4-androstadiene-3,17-dione + FADH2
-
-
-
-
r
4-androstene-3,17-dione + FAD
1,4-androstadiene-3,17-dione + FADH2
-
-
-
-
r
4-androstene-3,17-dione + FAD
1,4-androstadiene-3,17-dione + FADH2
-
-
-
?
4-androstene-3,17-dione + FAD
1,4-androstadiene-3,17-dione + FADH2
-
-
-
-
?
4-androstene-3,17-dione + FAD
1,4-androstadiene-3,17-dione + FADH2
-
-
-
-
?
4-androstene-3,17-dione + FAD
1,4-androstadiene-3,17-dione + FADH2
Mycolicibacterium neoaurum NwIB-01
-
-
-
?
4-androstene-3,17-dione + FAD
1,4-androstadiene-3,17-dione + FADH2
-
-
-
?
4-androstene-3,17-dione + FAD
1,4-androstadiene-3,17-dione + FADH2
-
-
-
?
9alpha-hydroxy-4-androstene-3,17-dione + acceptor
9alpha-hydroxy-1,4-androstadiene 3,17-dione + reduced acceptor
involved in steroid catabolism
-
-
?
9alpha-hydroxy-4-androstene-3,17-dione + acceptor
9alpha-hydroxy-1,4-androstadiene 3,17-dione + reduced acceptor
involved in steroid catabolism
-
-
?
additional information
?
-
enzyme works in an early step of steroid degradation
-
-
?
additional information
?
-
enzyme works in an early step of steroid degradation
-
-
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
FAD
-
contains 1 mol of flavin adenine dinucleotide per mol of protein
FAD
putative N-terminal FAD-binding motif in KsdDM is GSG(A/G)(A/G)(A/G)X17E
FAD
-
putative N-terminal FAD-binding motif in KsdDM is GSG(A/G)(A/G)(A/G)X17E
FAD
the C1 and C2 atoms of the substrate are at reaction distance to the N5 atom of the isoalloxazine ring of FAD and the hydroxyl group of Tyr318, respectively, whereas the C3 carbonyl group is at hydrogen bonding distance from the hydroxyl group of Tyr487 and the backbone amide of Gly491
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Ca2+
-
stimulates production of prednisolone, optimal activity in presence of 0.4 g/L
FeCl3
-
optimal concentration 0.06 g/l, 1.2-1.3fold increase of activity
K+
-
stimulates production of prednisolone, optimal activity in presence of 0.4 g/L
Mg2+
-
stimulates production of prednisolone, optimal activity in presence of 0.4 g/L
MnSO4
-
optimal concentration 0.06 g/l, 1.2-1.3fold increase of activity
Na+
-
stimulates production of prednisolone, optimal activity in presence of 0.6 g/L
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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
cortisol
-
optimal concentration is 0.1 mg/ml, increase of concentration to 1 mg/ml reduces prednisolone yield
additional information
-
not sensitive to carbonyl reagents and little sensitivity to metal chelating agents
-
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ACTIVATING COMPOUND
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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DISEASE
TITLE OF PUBLICATION
LINK TO PUBMED
Tuberculosis
3-Keto-5alpha-steroid Delta(1)-dehydrogenase from Rhodococcus erythropolis SQ1 and its orthologue in Mycobacterium tuberculosis H37Rv are highly specific enzymes that function in cholesterol catabolism.
Tuberculosis
The role of 3-ketosteroid 1(2)-dehydrogenase in the pathogenicity of Mycobacterium tuberculosis.
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KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
0.0246
(5alpha,17beta)-17-hydroxyandrostan-3-one
pH 7.0, 30°C
0.0064
11-deoxy-17alpha-hydroxycorticosterone
-
i.e. 4-pregnene-17alpha,21-diol-3,20-dione
0.0196
11alpha-hydroxyprogesterone
-
i.e. 4-pregnene-11alpha-ol-3,20-dione, 11beta-hydroxy-4-androstene-3,17-dione, 4-estren-3,17-dione
0.0063
11beta-hydroxytestosterone
-
i.e. 4-androstene-11beta,17beta-diol-3-one
0.0385
hydrocorticosterone
-
i.e. 4-pregnene-11beta,17alpha,21-triol-3,20-dione
0.0143
testololactone
-
i.e. 17alpha-oxo-D-homo-1,4-androstadiene-3,17-dione
0.064
11beta-cortisol
apparent value, in 50 mM Tris (pH 7.4), at 30°C
0.557
11beta-cortisol
apparent value, in 50 mM Tris (pH 7.4), at 30°C
0.3474
19alpha-hydroxy-4-androstene-3,17-dione
pH 7.0, 30°C
-
0.3688
19alpha-hydroxy-4-androstene-3,17-dione
pH 7.0, 30°C
-
0.026
23,24-bis-nor-5alpha-cholestan-3-one acid
apparent value, in 50 mM Tris (pH 7.4), at 30°C
0.074
23,24-bis-nor-5alpha-cholestan-3-one acid
apparent value, in 50 mM Tris (pH 7.4), at 30°C
0.011
4-androstene-17beta-ol-3-one
pH 7.0, 30°C
0.082
4-androstene-17beta-ol-3-one
pH 7.0, 30°C
0.085
4-androstene-17beta-ol-3-one
pH 7.0, 30°C
0.005
4-androstene-3,17-dione
apparent value, in 50 mM Tris (pH 7.4), at 30°C
0.104
4-androstene-3,17-dione
apparent value, in 50 mM Tris (pH 7.4), at 30°C
0.0382
4-pregnen-3-one-20beta-carboxylic acid
pH 7.0, 30°C
-
0.0761
4-pregnen-3-one-20beta-carboxylic acid
pH 7.0, 30°C
-
0.006
5alpha-androstan-3-one-17beta-ol
apparent value, in 50 mM Tris (pH 7.4), at 30°C
0.036
5alpha-androstan-3-one-17beta-ol
apparent value, in 50 mM Tris (pH 7.4), at 30°C
0.07
5alpha-androstan-3-one-17beta-ol
apparent value, in 50 mM Tris (pH 7.4), at 30°C
0.166
5alpha-androstan-3-one-17beta-ol
-
apparent value, in 50 mM Tris (pH 7.4), at 30°C
0.035
5alpha-androstane-17beta-ol-3-one
pH 7.0, 30°C
0.0379
5alpha-androstane-17beta-ol-3-one
pH 7.0, 30°C
0.013
5alpha-androstane-3,17-dione
apparent value, in 50 mM Tris (pH 7.4), at 30°C
0.033
5alpha-androstane-3,17-dione
-
apparent value, in 50 mM Tris (pH 7.4), at 30°C
0.036
5alpha-androstane-3,17-dione
apparent value, in 50 mM Tris (pH 7.4), at 30°C
0.059
5alpha-androstane-3,17-dione
apparent value, in 50 mM Tris (pH 7.4), at 30°C
0.007
5alpha-pregnane-3,20-dione
apparent value, in 50 mM Tris (pH 7.4), at 30°C
0.014
5alpha-pregnane-3,20-dione
apparent value, in 50 mM Tris (pH 7.4), at 30°C
0.021
5alpha-pregnane-3,20-dione
apparent value, in 50 mM Tris (pH 7.4), at 30°C
0.0221
9alpha-hydroxy-4-androstene-3,17-dione
pH 7.0, 30°C
0.025
9alpha-hydroxy-4-androstene-3,17-dione
pH 7.0, 30°C
0.045
9alpha-hydroxy-4-androstene-3,17-dione
pH 7.0, 30°C
0.058
9alpha-hydroxy-4-androstene-3,17-dione
apparent value, in 50 mM Tris (pH 7.4), at 30°C
0.105
9alpha-hydroxy-4-androstene-3,17-dione
pH 7.0, 30°C
0.119
9alpha-hydroxy-4-androstene-3,17-dione
apparent value, in 50 mM Tris (pH 7.4), at 30°C
0.5432
9alpha-hydroxy-4-androstene-3,17-dione
pH 7.0, 30°C
0.003
progesterone
apparent value, in 50 mM Tris (pH 7.4), at 30°C
0.018
progesterone
apparent value, in 50 mM Tris (pH 7.4), at 30°C
0.029
progesterone
apparent value, in 50 mM Tris (pH 7.4), at 30°C
0.065
progesterone
-
apparent value, in 50 mM Tris (pH 7.4), at 30°C
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TURNOVER NUMBER [1/s]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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kcat/KM VALUE [1/mMs-1]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
0.000015
-
intracellular, soluble, recombinant enzyme, in 0.86 M Tris-HCl (pH9.0), 150 mM phenazine methosulfate, at 25°C, using 4-androstene-3,17-dione as substrate
0.000111
-
exracellular, soluble, recombinant enzyme, in 0.86 M Tris-HCl (pH9.0), 150 mM phenazine methosulfate, at 25°C, using 4-androstene-3,17-dione as substrate
0.8
soluble recombinant His-tagged enzyme, cell extract, pH 7.0, 30°C
1.75
-
recombinant enzyme expressed in Bacillus subtilis, pH 7.0, 30°C
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pH OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
10
8 - 9
-
3-oxo-5beta-steroid DELTA1-dehydrogenase is active only on 5beta-steroids
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pH RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
7 - 10.5
-
pH 7: about 60% of activity maximum, pH 10.5: about 85% of activity maximum, phenazine methosulfate dehydrogenation
8 - 11
-
pH 8: about 35% of activity maximum, pH 11: about 25% of activity maximum, oxidase reaction
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TEMPERATURE OPTIMUM
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
30
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TEMPERATURE RANGE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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pI VALUE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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ORGANISM
COMMENTARY
LITERATURE
UNIPROT
SEQUENCE DB
SOURCE
3-oxo-5beta-steroid DELTA1-dehydrogenase is active only on 5-beta-steroids
-
-
brenda
three 3-ketosteroid DELTA1-dehydrogenase isoenzymes, gene kstD1 encodes isozyme DELTA1-KSTD1
UniProt
brenda
three 3-ketosteroid DELTA1-dehydrogenase isoenzymes, gene kstD1 encodes isozyme DELTA1-KSTD1
UniProt
brenda
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LOCALIZATION
ORGANISM
UNIPROT
COMMENTARY
GeneOntology No.
LITERATURE
SOURCE
-
partly, recombinant protein in Escherichia coli
brenda
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GENERAL INFORMATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
malfunction
metabolism
physiological function
malfunction
-
an enzyme-deficient mutant strain is unable to use cholesterol as a source of carbon and energy and has a limited ability to multiply. The mutant is unable to inhibit the NO and reactive oxygen species production induced through Toll-like receptor 2 signaling in infected resting macrophages, phenotpe, overview
malfunction
-
an enzyme-deficient mutant strain is unable to use cholesterol as a source of carbon and energy and has a limited ability to multiply. The mutant is unable to inhibit the NO and reactive oxygen species production induced through Toll-like receptor 2 signaling in infected resting macrophages, phenotpe, overview
-
metabolism
-
the 3-ketosteroid-DELTA1-dehydrogenase is involved in steroid metabolism catalyzing the transformation of 4-androstene-3,17-dione to androst-1,4-diene-3,17-dione specifically
metabolism
-
the 3-ketosteroid-DELTA1-dehydrogenase is involved in steroid metabolism catalyzing the transformation of 4-androstene-3,17-dione to androst-1,4-diene-3,17-dione specifically
-
physiological function
3-ketosteroid DELTA1-dehydrogenase plays a crucial role in the early steps of steroid degradation by introducing a double bond between the C1 and C2 atoms of the A-ring of its 3-ketosteroid substrates
physiological function
-
the enzyme functions in the conversion of 4-ene-3-oxosteroid to 1,4-diene-3-oxosteroid by trans-axial elimination of the Cl and C2 hydrogens, it is active in DELTA1-dehydrogenation in fusidane antibiotic biosynthesis, an important transformations in the synthesis of steroid hormones
physiological function
-
3-ketosteroid DELTA1-dehydrogenase plays a crucial role in the early steps of steroid degradation by introducing a double bond between the C1 and C2 atoms of the A-ring of its 3-ketosteroid substrates
-
physiological function
-
the enzyme functions in the conversion of 4-ene-3-oxosteroid to 1,4-diene-3-oxosteroid by trans-axial elimination of the Cl and C2 hydrogens, it is active in DELTA1-dehydrogenation in fusidane antibiotic biosynthesis, an important transformations in the synthesis of steroid hormones
-
Show AA Sequence and Transmembrane Helices (1439 entries)
Please use the AA Sequence and Transmembrane Helices Search for a specific query.
Please use the AA Sequence and Transmembrane Helices Search for a specific query.
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PDB
SCOP
CATH
UNIPROT
ORGANISM
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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
55000
55000
-
SDS-PAGE, recombinant protein, expression in Escherichia coli
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SUBUNIT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
monomer
monomer
-
1 * 55000, SDS-PAGE, PAGE, gel filtration, N-terminal sequence
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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
in complex with the reaction product 1,4-androstadiene-3,17-dione, to 2.3 A resolution. Key active site residues are Tyr119, Tyr318, Tyr487, and Gly491
sitting drop vapour diffusion method, mixing of 15 mg/ml protein in 25 mM bicine, pH 9.0, 100 mM NaCl, 10% v/v glycerol with 2% v/v PEG 400, 0.1 M, HEPES, pH 7.5, 2.0 M ammonium sulfate, 20°C, 5-7 days, method optimization, X-ray diffraction structure determination and analysis at 2.0 A resolution, multiwavelength anomalous dispersion analysis
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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
additional information
additional information
-
enzyme from strain ST-095 only differs in residue V366 from strain JC-12 (S366). Mutation results in almost 3fold higher catalytic efficiency in strain JC-12
additional information
-
enzyme from strain ST-095 only differs in residue V366 from strain JC-12 (S366). Mutation results in almost 3fold higher catalytic efficiency in strain JC-12
-
additional information
Mycolicibacterium neoaurum ST-095
-
enzyme from strain ST-095 only differs in residue V366 from strain JC-12 (S366). Mutation results in almost 3fold higher catalytic efficiency in strain JC-12
-
additional information
-
targeted disruption of the MSMEG5898 (ksdD-1) gene, but not the MSMEG4855 (ksdD-2) gene, results in partial inactivation of the cholesterol degradation pathway and accumulation of the intermediate 4-androstene-3,17-dione, reversible by the introduction of the wild-type ksdD-1 gene into deltaksdD-1 or overexpression of ksdD-2
additional information
Mycolicibacterium smegmatis mc(2)155 / ATCC 700084
-
targeted disruption of the MSMEG5898 (ksdD-1) gene, but not the MSMEG4855 (ksdD-2) gene, results in partial inactivation of the cholesterol degradation pathway and accumulation of the intermediate 4-androstene-3,17-dione, reversible by the introduction of the wild-type ksdD-1 gene into deltaksdD-1 or overexpression of ksdD-2
-
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pH STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
6 - 9
below pH 6.0 no apparent Tm is observed, indicating that DELTA1-KSTD1 is destabilized at low pH. The higher the pH value, within the range of pH 6.0-9.0, the higher the apparent Tm of DELTA1-KSTD1. At pH 9.0 the apparent Tm of DELTA1-KSTD1 is about 7 K higher than at pH 7.0
723868
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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
immobilized enzyme, adsorption on DEAE cellulose, half-life: 13-15 days
KSTD3 activity is lost upon purification using Ni-NTA column chromatography
immobilized enzyme, adsorption on DEAE cellulose, half-life: 13-15 days
-
immobilized enzyme, adsorption on DEAE cellulose, half-life: 13-15 days
Nocardia erythropolis
-
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ORGANIC SOLVENT
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
KSTD3 activity is lost upon purification using Ni-NTA column chromatography
recombinant enzyme from Pichia pastoris GS115 by hydrophobic interaction chromatography, recombinant His-tagged enzyme from Escherichia coli strain BL21(lambdaDE3) by nickel affinity chromatography
-
recombinant His-tagged DELTA1-KSTD1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
recombinant His-tagged enzyme by nickel affinity chromatography
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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expression in Escherichia coli
gene ksdD, phylogenetic analysis, expression in Bacillus subtilis strain 168 with 18fold higher activity compared to wild-type Mycobacterium neoaurum strain JC-12
-
gene kstD, DNA and amino acid sequence determination and analysis, phylogenetic tree, heterologous expression of the His-tagged enzyme, by low-temperature induction at 22°C, the recombinant protein appears partly as soluble protein
gene kstD1, heterologous expression of the His-tagged DELTA1-KSTD1 protein in Escherichia coli strain BL21(DE3)
gene kstDF, DNA and amino acid sequence determination, analysis, and comparison, expression in Escherichia coli strain BL21(lambdaDE3) and in Pichia pastoris strains KM71 and GS115 cytoplasm, both intracellularly and extracelluarly, the latter is inactive, method optimization
-
into the pJam2 expression vector under the acetamidase promoter and introduced into the deltaksdD-1 mutant
-
site directed mutagenesis of steroid binding site, Y-121 involved in catalytic function, Y-116, Y-104 involved in substrate binding
-
under control of the Phsp promoter and introduced into mutant cells of Mycobacterium smegmatis
-
unmarked gene deletion, biochemical evidence for a second 3-ketosteroid-DELTA1-dehydrogenase
-
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EXPRESSION
ORGANISM
UNIPROT
LITERATURE
construction of a mutant Mtb H37Rv strain containing an inactivated kstD gene, DELTAkstD, which encodes 3-ketosteroid 1(2)-dehydrogenase by homologous recombination-based gene-replacement technique. Replication of mutant Mycobacterium tuberculosis is attenuated in resting human macrophages compared to the wild-type or complemented strains. The mutant is unable to inhibit the NO and reactive oxygen species production induced through Toll-like receptor 2 signaling in infected resting macrophages. But mutant and wild-type Mycobacterium tuberculosis behave similarly in macrophages activated with IFN-gamma before and during infection. The mutant is unable to use cholesterol as a source of carbon and energy and has a limited ability to multiply
construction of a mutant Mtb H37Rv strain containing an inactivated kstD gene, DELTAkstD, which encodes 3-ketosteroid 1(2)-dehydrogenase by homologous recombination-based gene-replacement technique. Replication of mutant Mycobacterium tuberculosis is attenuated in resting human macrophages compared to the wild-type or complemented strains. The mutant is unable to inhibit the NO and reactive oxygen species production induced through Toll-like receptor 2 signaling in infected resting macrophages. But mutant and wild-type Mycobacterium tuberculosis behave similarly in macrophages activated with IFN-gamma before and during infection. The mutant is unable to use cholesterol as a source of carbon and energy and has a limited ability to multiply
-
construction of a mutant Mtb H37Rv strain containing an inactivated kstD gene, DELTAkstD, which encodes 3-ketosteroid 1(2)-dehydrogenase by homologous recombination-based gene-replacement technique. Replication of mutant Mycobacterium tuberculosis is attenuated in resting human macrophages compared to the wild-type or complemented strains. The mutant is unable to inhibit the NO and reactive oxygen species production induced through Toll-like receptor 2 signaling in infected resting macrophages. But mutant and wild-type Mycobacterium tuberculosis behave similarly in macrophages activated with IFN-gamma before and during infection. The mutant is unable to use cholesterol as a source of carbon and energy and has a limited ability to multiply
-
-
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APPLICATION
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
synthesis
additional information
synthesis
engineered enzyme mutants, in which KsdDM is inactivated or augmented, are useful for production of phytosterols 4-androstene-3,17-dione or 1,4-androstadiene-3,17-dione, circumventing the difficulty of separating 4-androstene-3,17-dione from 1,4-androstadiene-3,17-dione, a key bottleneck to the microbial transformation of phytosterols in industry
synthesis
-
kstDF is a promising enzyme in steroid DELTA1-dehydrogenation and steroid reduction that is propitious to construct genetically engineered steroid-transforming recombinants by heterologous overexpression
synthesis
inactivation of KstD isoforms 1-3 leads to synthesis of about 6.02g/l of 9alpha-hydroxy-4-androstene-3,17-dione from 15 g/l of phytosterols. The product is mixed with 1.55 g/l of 4-androstene-3,17-dione as a major byproduct. Overexpression of the oxygenase component of 3-ketosteroid-9alpha-hydroxylase kshA, results in a yield of 9alpha-hydroxy-4-androstene-3,17-dione of 7.33 g/l
synthesis
-
kstDF is a promising enzyme in steroid DELTA1-dehydrogenation and steroid reduction that is propitious to construct genetically engineered steroid-transforming recombinants by heterologous overexpression
-
synthesis
Mycolicibacterium neoaurum NwIB-01
-
engineered enzyme mutants, in which KsdDM is inactivated or augmented, are useful for production of phytosterols 4-androstene-3,17-dione or 1,4-androstadiene-3,17-dione, circumventing the difficulty of separating 4-androstene-3,17-dione from 1,4-androstadiene-3,17-dione, a key bottleneck to the microbial transformation of phytosterols in industry
-
synthesis
-
inactivation of KstD isoforms 1-3 leads to synthesis of about 6.02g/l of 9alpha-hydroxy-4-androstene-3,17-dione from 15 g/l of phytosterols. The product is mixed with 1.55 g/l of 4-androstene-3,17-dione as a major byproduct. Overexpression of the oxygenase component of 3-ketosteroid-9alpha-hydroxylase kshA, results in a yield of 9alpha-hydroxy-4-androstene-3,17-dione of 7.33 g/l
-
additional information
-
ksdD-1 and ksdD-2 display respectively high (78%) and low (33%) amino acid sequence identity with the putative ksdD gene of Mycobacterium tuberculosis
additional information
-
the two putative Mycobacterium tuberculosis KsdDs, MT3641 and MT0809, complement the Mycobacterium smegmatis deltaksdD-1 deltaksdD-2 double mutant
additional information
Mycolicibacterium smegmatis mc(2)155 / ATCC 700084
-
ksdD-1 and ksdD-2 display respectively high (78%) and low (33%) amino acid sequence identity with the putative ksdD gene of Mycobacterium tuberculosis
-
additional information
Mycobacterium tuberculosis H37Ra / ATCC 25177
-
the two putative Mycobacterium tuberculosis KsdDs, MT3641 and MT0809, complement the Mycobacterium smegmatis deltaksdD-1 deltaksdD-2 double mutant
-
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REF.
AUTHORS
TITLE
JOURNAL
VOL.
PAGES
YEAR
ORGANISM (UNIPROT)
PUBMED ID
SOURCE
Itagaki, E.; Wakabayashi, T.; Hatta, T.
Purification and characterization of 3-ketosteroid-delta 1-dehydrogenase from Nocardia corallina
Biochim. Biophys. Acta
1038
60-67
1990
Gordonia rubripertincta
brenda
Atrat, P.; Deppmeyer, V.; Hörhold, C.
Efficient purification of a microbial steroid-1-dehydrogenase by electrophoretic desorption from the affinity matrix on a preparative scale
J. Chromatogr.
189
279-283
1980
Rhodococcus opacus
-
brenda
Groh, H.; Komel, R.; Deppmeyer, V.; Atrat, P.; Hörhold, C.
Steroid-transforming enzymes from microorganisms. XI. Reversibility of the dehydrogenation reaction of the steroid-1-dehydrogenase from Nocardia opaca
Z. Allg. Mikrobiol.
19
727-730
1979
Rhodococcus opacus
brenda
Itagaki, E.; Hatta, T.; Wakabayashi, T.; Suzuki, K.
Spectral properties of 3-ketosteroid-delta 1-dehydrogenase from Nocardia corallina
Biochim. Biophys. Acta
1040
281-286
1990
Gordonia rubripertincta
brenda
Kloosterman IV, J.; Lilly, M.D.
Effect of supersaturated aqueous hydrocortison concentrations on the DELTA1-dehydrogenase activity of free and immobilized Arthrobacter simplex
Enzyme Microb. Technol.
6
113-116
1984
Pimelobacter simplex
-
brenda
Penasse, L.; Nomine, G.
The active center of the 3-oxosteroid delta-1-dehydrogenase from Arthrobacter simplex
Eur. J. Biochem.
47
555-559
1974
Pimelobacter simplex
brenda
Aries, V.C.; Goddard, P.; Hill, M.J.
Degradation of steroids by intestinal bacteria. III. 3-Oxo-5beta-steroid DELTA1-dehydrogenase and 3-oxo-5beta-steroid DELTA4-dehydrogenase
Biochim. Biophys. Acta
248
482-488
1971
Clostridium paraputrificum
-
brenda
Rodig, O.R.; Roller, P.P.; Nicholas, A.W.
The stereochemistry of hydrogen addition by a delta1-steroid reductase in bakers yeast
Biochem. Biophys. Res. Commun.
56
467-470
1974
Saccharomyces cerevisiae
brenda
Levy, H.R.; Talalay, P.
Bacterial oxidation of steroids. II. Studies on the enzymatic mechanism of ring A dehydrogenation
J. Biol. Chem.
234
2014-2021
1959
Comamonas testosteroni
brenda
Atrat, P.; Groh, H.
Steroid transformation with immobilized microorganisms. VI. The reverse reaction of steroid-1-dehydrogenases from different micoorganisms in immobilized state
Z. Allg. Mikrobiol.
21
3-6
1981
Mycolicibacterium phlei, Nocardia erythropolis, Rhodococcus opacus
brenda
Udvardy, E.N.; Hantos, G.
Kinetics of the formation of steroid-specific enzymes
FEMS Symp.
13
21-33
1982
Pimelobacter simplex
-
brenda
Hörhold, C.; Atrat, P.; Deppmeyer, V.; Gabert, A.; Flemming, C.; Schubert, K.
Steroid-transforming enzymes from microorganisms. II. Purification of a 3-oxosteroid: (2,6-dichlorophenolindophenol) delta 1-oxidoreductase from Nocardia opaca by means of affinity chromatography
Z. Allg. Mikrobiol.
16
559-561
1976
Rhodococcus opacus
brenda
Lestrovaja, N.N.; Dänhardt, S.; Hörhold, C.
Steroid transforming enzymes from microorganisms. V. Purification of a 4-en-3-oxosteroid: (acceptor)-1-en-oxidoreductase from Nocardia opaca and characterization of the prosthetic group
Z. Allg. Mikrobiol.
18
189-196
1978
Rhodococcus opacus
brenda
Hörhold, C.; Huller, E.; Rose, G.
Steroid-transforming enzymes from microorganisms. XII. Induction characteristics of the 4-en-3-oxosteroid: (acceptor)-1-en-oxidoreductase in Nocardia opaca
Z. Allg. Mikrobiol.
19
731-739
1979
Rhodococcus opacus
brenda
Hörhold, C.; Huller, E.; Rose, G.
Steroid transforming enzymes in microorganisms. XIII. Relationship between steroid structure and induction of 4-ene-3-oxosteroid: (acceptor)-1-ene-oxidoreductase in Nocardia opaca
Z. Allg. Mikrobiol.
20
23-32
1980
Rhodococcus opacus
brenda
Abul-Hajj, Y.J.
Isolation of vitamin K2(35) from Nocardia restrictus and Corynebacterium simplex. A natural electron acceptor in microbial steroid ring A dehydrogenations
J. Biol. Chem.
253
2356-2360
1978
Pimelobacter simplex, Rhodococcus hoagii, Ilyonectria destructans, Plectosphaerella cucumerina
brenda
Van der Geize, R.; Hessels, G.I.; van Gerwen, R.; van der Meijden, P.; Dijkhuizen, L.
Unmarked gene deletion mutagenesis of kstD, encoding 3-ketosteroid DELTA1-dehydrogenase, in Rhodococcus erythropolis SQ1 using sacB as counter-selectable marker
FEMS Microbiol. Lett.
205
197-202
2001
Rhodococcus erythropolis
brenda
Choi, K.P.; Molnar, I.; Yamashita, M.; Murooka, Y.
Purification and characterization of the 3-ketosteroid-DELTA1-dehydrogenase of Arthrobacter simplex produced in Streptomyces lividans
J. Biochem.
117
1043-1049
1995
Pimelobacter simplex
brenda
Choi, K.P.; Molnar, I.; Murooka, Y.
Secretory overproduction of Arthrobacter simplex 3-ketosteroid DELTA1-dehydrogenase by Streptomyces lividans with a multi-copy shuttle vector
Appl. Microbiol. Biotechnol.
43
1044-1049
1995
Pimelobacter simplex
brenda
Fujii, C.; Morii, S.; Kadode, M.; Sawamoto, S.; Iwami, M.; Itagaki, E.
Essential tyrosine residues in 3-ketosteroid-DELTA1-dehydrogenase from Rhodococcus rhodochrous
J. Biochem.
126
662-667
1999
Rhodococcus rhodochrous
brenda
Molnar, I.; Choi, K.P.; Yamashita, M.; Murooka, Y.
Molecular cloning, expression in Streptomyces lividans, and analysis of a gene cluster from Arthrobacter simplex encoding 3-ketosteroid-DELTA1-dehydrogenase, 3-ketosteroid-DELTA5-isomerase and a hypothetical regulatory protein
Mol. Microbiol.
15
895-905
1995
Pimelobacter simplex
brenda
Morii, S.; Fujii, C.; Miyoshi, T.; Iwami, M.; Itagaki, E.
3-Ketosteroid-DELTA1-dehydrogenase of Rhodococcus rhodochrous: sequencing of the genomic DNA and hyperexpression, purification, and characterization of the recombinant enzyme
J. Biochem.
124
1026-1032
1998
Rhodococcus rhodochrous
brenda
Horinouchi, M.; Hayashi, T.; Yamamoto, T.; Kudo, T.
A new bacterial steroid degradation gene cluster in Comamonas testosteroni TA441 which consists of aromatic-compound degradation genes for seco-steroids and 3-ketosteroid dehydrogenase genes
Appl. Environ. Microbiol.
69
4421-4430
2003
Comamonas testosteroni (Q06401), Comamonas testosteroni TA441 (Q06401)
brenda
van der Geize, R.; Hessels, G.I.; Dijkhuizen, L.
Molecular and functional characterization of the kstD2 gene of Rhodococcus erythropolis SQ1 encoding a second 3-ketosteroid DELTA(1)-dehydrogenase isoenzyme
Microbiology
148
3285-3292
2002
Rhodococcus erythropolis (Q8RLT5), Rhodococcus erythropolis SQ1 (Q8RLT5), Rhodococcus erythropolis SQ1
brenda
Adham, N.Z.; El-Hady, A.A.; Naim, N.
Biochemical studies on the microbial DELTA1-dehydrogenation of cortisol by Pseudomonas fluorescens
Proc. Biochem.
38
897-902
2003
Pseudomonas fluorescens
-
brenda
Brzostek, A.; Sliwinski, T.; Rumijowska-Galewicz, A.; Korycka-Machala, M.; Dziadek, J.
Identification and targeted disruption of the gene encoding the main 3-ketosteroid dehydrogenase in Mycobacterium smegmatis
Microbiology
151
2393-2402
2005
Mycobacterium tuberculosis, Mycolicibacterium smegmatis, Mycolicibacterium smegmatis mc(2)155 / ATCC 700084, Mycobacterium tuberculosis H37Ra / ATCC 25177
brenda
Knol, J.; Bodewits, K.; Hessels, G.I.; Dijkhuizen, L.; van der Geize, R.
3-Keto-5alpha-steroid DELTA(1)-dehydrogenase from Rhodococcus erythropolis SQ1 and its orthologue in Mycobacterium tuberculosis H37Rv are highly specific enzymes that function in cholesterol catabolism
Biochem. J.
410
339-346
2008
Mycobacterium tuberculosis, Rhodococcus erythropolis (A9QAE5), Rhodococcus erythropolis (Q8RLT5), Rhodococcus erythropolis (Q9RA02), Rhodococcus erythropolis, Rhodococcus erythropolis SQ1 (A9QAE5), Rhodococcus erythropolis SQ1 (Q8RLT5), Rhodococcus erythropolis SQ1 (Q9RA02), Rhodococcus erythropolis SQ1, Mycobacterium tuberculosis H37Rv
brenda
Li, Y.; Lu, F.; Sun, T.; Du, L.
Expression of ksdD gene encoding 3-ketosteroid-DELTA1-dehydrogenase from Arthrobacter simplex in Bacillus subtilis
Lett. Appl. Microbiol.
44
563-568
2007
Pimelobacter simplex
brenda
Rohman, A.; van Oosterwijk, N.; Dijkstra, B.W.
Purification, crystallization and preliminary X-ray crystallographic analysis of 3-ketosteroid DELTA1-dehydrogenase from Rhodococcus erythropolis SQ1
Acta Crystallogr. Sect. F
68
551-556
2012
Rhodococcus erythropolis (Q9RA02), Rhodococcus erythropolis SQ1 (Q9RA02)
brenda
Wei, W.; Wang, F.Q.; Fan, S.Y.; Wei, D.Z.
Inactivation and augmentation of the primary 3-ketosteroid-DELTA1-dehydrogenase in Mycobacterium neoaurum NwIB-01: biotransformation of soybean phytosterols to 4-androstene-3,17-dione or 1,4-androstadiene-3,17-dione
Appl. Environ. Microbiol.
76
4578-4582
2010
Mycolicibacterium neoaurum (C6KID9), Mycolicibacterium neoaurum NwIB-01 (C6KID9)
brenda
Chen, M.M.; Wang, F.Q.; Lin, L.C.; Yao, K.; Wei, D.Z.
Characterization and application of fusidane antibiotic biosynethsis enzyme 3-ketosteroid-DELTA1-dehydrogenase in steroid transformation
Appl. Microbiol. Biotechnol.
96
133-142
2012
Aspergillus fumigatus, Aspergillus fumigatus CICC 40167
brenda
Brzezinska, M.; Szulc, I.; Brzostek, A.; Klink, M.; Kielbik, M.; Sulowska, Z.; Pawelczyk, J.; Dziadek, J.
The role of 3-ketosteroid 1(2)-dehydrogenase in the pathogenicity of Mycobacterium tuberculosis
BMC Microbiol.
13
43
2013
Mycobacterium tuberculosis, Mycobacterium tuberculosis H37Rv
brenda
Zhang, W.; Shao, M.; Rao, Z.; Xu, M.; Zhang, X.; Yang, T.; Li, H.; Xu, Z.
Bioconversion of 4-androstene-3,17-dione to androst-1,4-diene-3,17-dione by recombinant Bacillus subtilis expressing ksdd gene encoding 3-ketosteroid-DELTA1-dehydrogenase from Mycobacterium neoaurum JC-12
J. Steroid Biochem. Mol. Biol.
135
36-42
2013
Mycolicibacterium neoaurum, Mycolicibacterium neoaurum JC-12
brenda
Rohman, A.; van Oosterwijk, N.; Thunnissen, A.M.; Dijkstra, B.W.
Crystal structure and site-directed mutagenesis of 3-ketosteroid Delta1-dehydrogenase from Rhodococcus erythropolis SQ1 explain its catalytic mechanism
J. Biol. Chem.
288
35559-35568
2013
Rhodococcus erythropolis (Q9RA02), Rhodococcus erythropolis SQ1 (Q9RA02)
brenda
Shao, M.; Zhang, X.; Rao, Z.; Xu, M.; Yang, T.; Li, H.; Xu, Z.; Yang, S.
A mutant form of 3-ketosteroid-Delta(1)-dehydrogenase gives altered androst-1,4-diene-3, 17-dione/androst-4-ene-3,17-dione molar ratios in steroid biotransformations by Mycobacterium neoaurum ST-095
J. Ind. Microbiol. Biotechnol.
43
691-701
2016
Mycolicibacterium neoaurum, Mycolicibacterium neoaurum JC-12, Mycolicibacterium neoaurum ST-095
brenda
Yao, K.; Xu, L.Q.; Wang, F.Q.; Wei, D.Z.
Characterization and engineering of 3-ketosteroid-Delta1-dehydrogenase and 3-ketosteroid-9alpha-hydroxylase in Mycobacterium neoaurum ATCC 25795 to produce 9alpha-hydroxy-4-androstene-3,17-dione through the catabolism of sterols
Metab. Eng.
24
181-191
2014
Mycolicibacterium neoaurum (C8CJG6), Mycolicibacterium neoaurum (W0NR13), Mycolicibacterium neoaurum (W0NUN5), Mycolicibacterium neoaurum ATCC 25795 (C8CJG6), Mycolicibacterium neoaurum ATCC 25795 (W0NR13), Mycolicibacterium neoaurum ATCC 25795 (W0NUN5)
brenda
Guevara, G.; Fernandez de Las Heras, L.; Perera, J.; Navarro Llorens, J.M.
Functional differentiation of 3-ketosteroid Delta(1)-dehydrogenase isozymes in Rhodococcus ruber strain Chol-4
Microb. Cell Fact.
16
42
2017
Rhodococcus ruber (C6GCU2), Rhodococcus ruber (I0B6I6), Rhodococcus ruber (I0B6J0), Rhodococcus ruber, Rhodococcus ruber Chol-4 (C6GCU2), Rhodococcus ruber Chol-4 (I0B6I6), Rhodococcus ruber Chol-4 (I0B6J0)
brenda
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