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EC Number Cloned (Commentary) Reference
Show all pathways known for 1.1.1.100Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.100overexpressed in Escherichia coli BL21 (DE3) 704843
Show all pathways known for 1.1.1.100Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.100overexpression in Escherichia coli strain BL21 (DE3) 285682
Show all pathways known for 1.1.1.100Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.100overexpression of fabG gene in Escherichia coli strain BL21 (DE3) 285681
Show all pathways known for 1.1.1.100Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.100overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) in inclusion bodies 670774
Show all pathways known for 1.1.1.100Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.100PCR product recombined with pDONR221 and insert from this vector transferred in the LR reaction to the expression vector pET15g which adds a histidine tag and a 3C protease cleavage site, expressed in Escherichia coli B834(DE3) cells 706640
Show all pathways known for 1.1.1.100Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.100pET15b-fabG vector construct transformed into the Escherichia coli BL21 DE3 strain 703163
Show all pathways known for 1.1.1.100Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.100Recombinant Escherichia coli (strain DH5alpha) co-expresses the fabG gene. The fabG gene is PCR-cloned using primers derived from the up and down-stream regions of the Pseudomonas putida KT2440 genome. 693287
Show all pathways known for 1.1.1.100Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.100recombinant hexahistidine-tagged FabG2 is expressed in Escherichia coli 763418
Show all pathways known for 1.1.1.100Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.100selection of an Escherichia coli, BL21(DE3)NH host that stably expresses mutant forms of 3-ketoacyl-ACP(CoA) reductase 657270
Show all pathways known for 1.1.1.100Display the word mapDisplay the reaction diagram Show all sequences 1.1.1.100transformation of the fabG gene encoding 3-ketoacyl-[acyl-carrier-protein] reductase in fusion with a His6-MBP-tag (ligated into vector pETM-41) into Escherichia coli XL-10 Gold. After isolation from XL-10 cells and DNA sequencing, transformed into Escherichia coli Tuner (DE3) cells 701783
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