1.1.1.100 - 763622 1.1.1.100 C-terminal His-tagged FabG from Staphylococcus aureus is cloned into the NcoI and XhoI cloning sites of the vector pET28a, expressed in BL21 DE3 Escherichia coli 763086 1.1.1.100 coexpression in Escherichia coli LS5218 with fabH mutant F87T and polyhydroxyalkanoate synthase genes 685775 1.1.1.100 complementation of Escherichia coli fabG mutant with Lactobacillus lactis fabG genes shows that fabG1 encodes a functional beta-ketoacyl ACP reductase and fabG2 encodes an enzyme that has activity with the model substrate beta-ketobutyryl-CoA, but lacks the ability to support fatty acid synthesis both in vivo and in vitro 654725 1.1.1.100 expressed in Escherichia coli 724676 1.1.1.100 expressed in Escherichia coli as a His-tagged fusion protein 723843 1.1.1.100 expressed in Escherichia coli BL21 (RARE) cells 741044 1.1.1.100 expressed in Escherichia coli BL21 Rosetta 2 (DE3) pLysS cells 740023, 762566 1.1.1.100 expressed in Escherichia coli BL21(DE3) cells 740558 1.1.1.100 expressed in Escherichia coli BL21(DE3) cells and in Corynebacterium glutamicum 739901 1.1.1.100 expressed in Escherichia coli BL21-CodonPlus(DE3)-RIPL cells 740630 1.1.1.100 expression in Escherichia coli 285680, 654432, 684165, 684702, 689973 1.1.1.100 expression in Saccharomyces cerevisae mutant cells lacking the homologous mitochondrial FASII enzyme 3-oxoacyl-ACP reductase Oar1p 704721 1.1.1.100 expression in Streptomyces cerevisiae cells lacking 3-oxoacyl-ACP reductase 705747 1.1.1.100 expression of protein without transit peptide in Escherichia coli 285679 1.1.1.100 FabG1 expressed from vector pPLM189 as mitochondrial protein in the Saccharomyces cerevisiae oar1DELTA mutant strain. FabG1 can restore respiratory growth of Saccharomyces cerevisiae oar1DELTA cells and renews lipoic acid production 705747 1.1.1.100 FabG4 expressed from vector pPLM192 as mitochondrial protein in the Saccharomyces cerevisiae oar1DELTA mutant strain. FabG4 can restore respiratory growth of Saccharomyces cerevisiae oar1DELTA cells and renews lipoic acid production 705747 1.1.1.100 gene fabG, DNA and amino acid sequence determination and analysis, expression of C-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) 668574 1.1.1.100 gene fabG, DNA and amino acid sequence determination and analysis, expression of His10-tagged enzyme in Escherichia coli 667520 1.1.1.100 gene fabG, expression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) 669013 1.1.1.100 gene fabG, overexpression of His-tagged enzyme in strain BL21(DE3) 667257 1.1.1.100 gene fabG, overexpression of His-tagged enzyme in strain BL21(DE3), recombinant expression in Escherichia coli strain JM109 leads to enhanced production of polyhydroxyalkanoate, PHA, copolymer from glucose, PHA composition, overview 667257 1.1.1.100 gene rhlG, expression of His-tagged enzyme in Escherichia coli strain BL21(DE3) or, as selenomethionine-labeled variant in Escherichia coli strain B834 in minimal medium 669470 1.1.1.100 heterologous coexpression of the polyhydroxyalkanoate synthase genes phaEC together with fabG1 in Haloferax volcanii 701808 1.1.1.100 heterologous coexpression of the polyhydroxyalkanoate synthase genes phaEC together with Haloarcula hispanica fabG1 reconstructs the polyhydroxyalkanoate biosynthetic pathway 701808 1.1.1.100 His-tagged FabG expressed in Escherichia coli strain BL21 (DE3) 706142 1.1.1.100 into pET-28a vector and expressed in Escherichia coli BL21 (DE3) codon plus 706639 1.1.1.100 N-terminal 6 × His-tagged Xcc FabG3 recombinant protein is expressed in Escherichia coli BL21 (DE3) 763477 1.1.1.100 N-terminal His-tagged FabG from Escherichia coli is cloned into the NcoI and XhoI cloning sites of the vector pET28a, expressed in BL21 DE3 Escherichia coli 763086 1.1.1.100 NH2-terminally His-tagged FabG expressed in Escherichia coli strain BL21(DE3) 704337 1.1.1.100 overexpressed in Escherichia coli BL21 (DE3) 704843 1.1.1.100 overexpression in Escherichia coli strain BL21 (DE3) 285682 1.1.1.100 overexpression of fabG gene in Escherichia coli strain BL21 (DE3) 285681 1.1.1.100 overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3) in inclusion bodies 670774 1.1.1.100 PCR product recombined with pDONR221 and insert from this vector transferred in the LR reaction to the expression vector pET15g which adds a histidine tag and a 3C protease cleavage site, expressed in Escherichia coli B834(DE3) cells 706640 1.1.1.100 pET15b-fabG vector construct transformed into the Escherichia coli BL21 DE3 strain 703163 1.1.1.100 Recombinant Escherichia coli (strain DH5alpha) co-expresses the fabG gene. The fabG gene is PCR-cloned using primers derived from the up and down-stream regions of the Pseudomonas putida KT2440 genome. 693287 1.1.1.100 recombinant hexahistidine-tagged FabG2 is expressed in Escherichia coli 763418 1.1.1.100 selection of an Escherichia coli, BL21(DE3)NH host that stably expresses mutant forms of 3-ketoacyl-ACP(CoA) reductase 657270 1.1.1.100 transformation of the fabG gene encoding 3-ketoacyl-[acyl-carrier-protein] reductase in fusion with a His6-MBP-tag (ligated into vector pETM-41) into Escherichia coli XL-10 Gold. After isolation from XL-10 cells and DNA sequencing, transformed into Escherichia coli Tuner (DE3) cells 701783 1.1.1.100 transformation with 3-oxoacyl-[acyl-carrier-protein] reductase in antisense orientation, driven by either the cauliflower mosaic virus 35S promoter or a seed-specific acyl carrier protein promoter 689500 1.1.1.100 wild-type and mutants cloned into the pET-28a(+) vector and expressed in Escherichia coli BL21 (DE3) codon plus 703630 1.1.1.100 YFP-KCR1 and CFP-KCR2 fusion constructs transiently expressed in tobacco under the control of the 35S promoter or in Arabidopsis. KCR1 and KCR2 expressed in the yeast ybr159DELTA mutant 700827