EC Number   |
Substrates |
Organism  |
Products |
Reversibility |
|---|
   4.6.1.16 | more |
Aeropyrum pernix expresses precursor-tRNAs with canonical or non-canonical introns at various positions. APE splicing endonuclease removes both types of introns, including the non-canonical introns, without any nucleotide modification. E.g. three tRNAThr species are transcribed and subsequently matured to functional tRNAs. During maturation, introns in two of them are cleaved from standard and non-standard positions, APE-EndA has broad substrate specificity, overview |
Aeropyrum pernix |
? |
- |
? |
| 4.6.1.16 | more |
Sulfolobus tokodaii tRNATrp precursor as a substrate |
Aeropyrum pernix |
? |
- |
? |
| 4.6.1.16 | more |
structural requirements of substrate |
Arabidopsis thaliana |
? |
- |
? |
| 4.6.1.16 | tRNA precursor |
- |
Arabidopsis thaliana |
a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus |
- |
? |
| 4.6.1.16 | 5'-AAGCGACCGACC*AUA*GCUGCA-3' + H2O |
- |
Archaeoglobus fulgidus |
? |
- |
? |
| 4.6.1.16 | 5'-AAUGCAGCGGUC*AAA*GGUCGC-3' + H2O |
- |
Archaeoglobus fulgidus |
? |
- |
? |
| 4.6.1.16 | more |
the enzyme cleaves tRNA substrates containing bulge-helix-bulge, BHB, motifs, but not bulge-helix-loop, BHL, motifs, specificity for noncanonical and canonical motifs, cis and trans splicing motifs, overview |
Archaeoglobus fulgidus |
? |
- |
? |
| 4.6.1.16 | more |
the enzyme cleaves two phosphodiester bonds within folded precursor RNAs during intron removal producing functional RNAs required for protein synthesis, the enzyme binds to bulge-helix-bulge motifs of RNA containing a noncleaved and a cleaved splice site, the enzyme the dimer recognizes cooperatively a flipped-out bulge base and stabilizes sharply bent bulge backbones that are poised for an in-line cleavage reaction |
Archaeoglobus fulgidus |
? |
- |
? |
| 4.6.1.16 | more |
the enzyme recognizes a unique RNA motif that consists of two three-nucleotide bulges separated by a four base-pair helix, known as the bulge-helix-bulge motif, BHB motif |
Archaeoglobus fulgidus |
? |
- |
? |
| 4.6.1.16 | more |
AFU-EndA has narrow substrate specificity, overview |
Archaeoglobus fulgidus |
? |
- |
? |
