EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
1.14.19.13 | (9Z,12Z)-hexadeca-9,12-dienoyl-CoA + reduced acceptor + O2 |
- |
Fusarium verticillioides |
(9Z,12Z)-hexadeca-9,12,15-trienoyl-CoA + acceptor + H2O |
- |
? |
1.14.19.13 | (9Z,12Z)-hexadeca-9,12-dienoyl-CoA + reduced acceptor + O2 |
the double bond introduced by the enzyme occurs between carbons 15 and 16 resulting in a terminal double bond aliphatic chain |
Sorghum bicolor |
(9Z,12Z)-hexadeca-9,12,15-trienoyl-CoA + acceptor + H2O |
- |
? |
1.14.19.13 | (9Z,12Z)-hexadeca-9,12-dienoyl-CoA + reduced acceptor + O2 |
- |
Rhodotorula kratochvilovae |
(9Z,12Z,15Z)-hexadeca-9,12,15-trienoyl-CoA + acceptor + 2 H2O |
- |
? |
1.14.19.13 | (9Z,12Z)-hexadeca-9,12-dienoyl-CoA + reduced acceptor + O2 |
- |
Rhodotorula kratochvilovae YM25235 |
(9Z,12Z,15Z)-hexadeca-9,12,15-trienoyl-CoA + acceptor + 2 H2O |
- |
? |
1.14.19.13 | more |
analysis of content in polyunsaturated and hydroxylated fatty acids in the cells, mass spectrometric analysis, overview |
Candida parapsilosis |
? |
- |
? |
1.14.19.13 | more |
the enzyme produces linoleic acid and alpha-linolenic acid, overview |
Rhodotorula kratochvilovae |
? |
- |
? |
1.14.19.13 | more |
the FAD3 enzyme is responsible for the DELTA15 desaturation of linoleic acid (18:2DELTA9,12) to alpha-linolenoic acid (18:3DELTA9,12,15) and of gamma-linolenic acid (18:3DELTA6,9,12) to stearidonic acid (18:4DELTA6,9,12,15). CSFAD3A acts as a DELTA15 desaturase in vivo as well as in a heterologous host |
Cannabis sativa |
? |
- |
? |
1.14.19.13 | more |
absence of desaturation activity of RKD12 for C16 substrates |
Rhodotorula kratochvilovae |
? |
- |
? |
1.14.19.13 | more |
recombinant CpFad3 produces DELTA15-polyunsaturated fatty acids, i.e., alpha-linolenic acid (DELTA9,DELTA12,DELTA15-18:3) and hexadecatrienoic acid with an unusual terminal double bond (DELTA9,DELTA12,DELTA15-16:3), identified by GC/MS analysis |
Candida parapsilosis |
? |
- |
? |
1.14.19.13 | more |
unambiguous identification of the triunsaturated product of CpFad2 as DELTA9,DELTA12,DELTA15-16:3 with terminal (n-1) double bond by comparing its spectrum to that of DELTA9,DELTA12,DELTA15-16:3 methylester. The major products of recombinant CpFad2 are linoleic and hexadecadienoic acid (DELTA9,DELTA12-16:2), accompanied by alpha-linolenic acid and hexadecatrienoic acid (DELTA9,DELTA12,DELTA15-16:3). Ricinoleic acid (12-hydroxy-9-octadecenoic acid) is an additional product of CpFad2 identified by GC/MS analysis |
Candida parapsilosis |
? |
- |
? |