EC Number |
Substrates |
Organism |
Products |
Reversibility |
---|
4.6.1.16 | tRNA precursor |
specifically removing the intron |
Saccharomyces cerevisiae |
a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus |
- |
? |
4.6.1.16 | tRNA precursor |
differences in distance and relative helical orientation of the splice sites in plant pre-tRNAs versus pre-tRNAs from other organisms are tolerated by the vertebrate splicing endonuclease, but not at all by the plant enzyme |
Homo sapiens |
a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus |
- |
? |
4.6.1.16 | tRNA precursor |
differences in distance and relative helical orientation of the splice sites in plant pre-tRNAs versus pre-tRNAs from other organisms are tolerated by the vertebrate splicing endonuclease, but not at all by the plant enzyme |
Triticum aestivum |
a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus |
- |
? |
4.6.1.16 | tRNA precursor |
- |
Saccharomyces cerevisiae M304 |
a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus |
- |
? |
4.6.1.16 | tRNA precursor |
endonucleolytic cleavage of pre-tRNA, producing 5'-hydroxyl and 2',3'-cyclic phosphate termini |
Saccharomyces cerevisiae M304 |
a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus |
- |
? |
4.6.1.16 | tRNA precursor |
specifically removing the intron |
Saccharomyces cerevisiae M304 |
a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus |
- |
? |
4.6.1.16 | tRNA precursor |
- |
Saccharomyces cerevisiae 20B-12-1 |
a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus |
- |
? |
4.6.1.16 | tRNA precursor |
cleaves pre-tRNA in a random order creating two routes for removal of the introns from pre-tRNA |
Saccharomyces cerevisiae 20B-12-1 |
a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus |
- |
? |
4.6.1.16 | tRNA precursor |
cleaves both 3'- and 5'-nicked pre-tRNAPhe |
Saccharomyces cerevisiae 20B-12-1 |
a 3'-half-tRNA molecule with a 5'-OH end + a 5'-half-tRNA molecule with a 2',3'-cyclic phosphate end + an intron with a 2',3'-cyclic phosphate and a 5'-hydroxyl terminus |
- |
? |