EC Number |
Storage Stability |
---|
1.2.1.3 | -20°C, 20% glycerol, less than 15% loss of activity per months |
1.2.1.3 | -20°C, all isoenzymes are stable for up to 1 month |
1.2.1.3 | -20°C, purified enzyme, 3 months, 52% loss of activity |
1.2.1.3 | -40°C, stable for several months |
1.2.1.3 | -70°C, liquid nitrogen, 6 months, the enzyme remains stable |
1.2.1.3 | -70°C, pH 6.0, 30 mM potassium phosphate, 1 mM EDTA, 1 nN dithiothreitol, 2 mM benzamidine, 70 mM MES, after half a year of storage with repeated freezing and thawing the activity of both ALDH-1 and ALDH-2 decrease 2fold to 3fold |
1.2.1.3 | -8°C, 16% glycerol, 1 mM dithiothreitol, 25% loss of activity after 6 months |
1.2.1.3 | 0°C, purified enzyme, 3 months, 46% loss of activity |
1.2.1.3 | 4°C, 10 mM phosphate buffer, pH 6.8, 1 mM EDTA, 0.1% v/v 2-mercaptoethanol, stable for a week |
1.2.1.3 | 4°C, 5% glycerol, 0.5 M NaCl, pH 7.5, no loss in activity after several months |