EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Reference |
---|
3.5.4.25 | -999 |
- |
activity of the mutant enzyme C54S, C65S, and C67S with the different substrates, overview, best substrate is GTP being cleaved to GMP |
655443 |
3.5.4.25 | 0.002 |
- |
purified recombinant mutant C65S, substrate GTP cleaved to GMP |
655443 |
3.5.4.25 | 0.003 |
- |
purified recombinant mutant C67S, substrate GTP cleaved to GMP |
655443 |
3.5.4.25 | 0.01 |
- |
purified recombinant mutant C54S, substrate GTP cleaved to GMP |
655443 |
3.5.4.25 | 0.015 |
- |
purified recombinant wild-type enzyme, substrate 2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone triphosphate cleaved to 2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate |
655443 |
3.5.4.25 | 0.016 |
- |
purified recombinant wild-type enzyme, substrate GTP cleaved to GMP |
655443 |
3.5.4.25 | 0.025 |
- |
purified recombinant wild-type enzyme, substrate dGTP cleaved to dGMP |
655443 |
3.5.4.25 | 0.038 |
- |
purified recombinant wild-type enzyme, substrate dGTP cleaved to 2,5-diamino-6-hydroxy-4-(5-phospho-deoxyribosylamino)pyrimidine |
655443 |
3.5.4.25 | 0.08 |
- |
recombinant enzyme |
685065 |
3.5.4.25 | 0.122 |
- |
purified recombinant wild-type enzyme, substrate 2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone triphosphate cleaved to 2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)pyrimidine |
655443 |