Literature summary for 3.5.4.25 extracted from

Spoonamore, J.E.; Dahlgran, A.L.; Jacobsen, N.E.; Bandarian, V.
Evolution of new function in the GTP cyclohydrolase II proteins of Streptomyces coelicolor (2006), Biochemistry, 45, 12144-12155.

Search for more literature for 3.5.4.25

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Streptomyces coelicolor

Protein Variants

Protein Variants	Comment	Organism
additional information	the M120T variant of GCH II/II SCO 6655 acquires the ability to catalyze the conversion of GTP to 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate	Streptomyces coelicolor

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Zn2+	contains 1 molar equivalent of zinc per mol of protein	Streptomyces coelicolor

Organism

Organism	UniProt	Comment	Textmining
Streptomyces coelicolor	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
DEAE-Sephadex column chromatography, Q-Sepharose column chromatography, butyl-Sepharose column chromatography, and Sephacryl S-300 gel filtration	Streptomyces coelicolor

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
0.08	-	recombinant enzyme	Streptomyces coelicolor

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
GTP + H2O	-	Streptomyces coelicolor	formate + 2,5-diamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate + diphosphate	-	?
GTP + H2O	-	Streptomyces coelicolor	2-amino-5-formylamino-6-ribosylamino-4(3H)-pyrimidinone 5'-phosphate + diphosphate + ?	the reaction product is exclusively formed by enzyme variant SCO 6655	?

Synonyms

Synonyms	Comment	Organism
GCH II	-	Streptomyces coelicolor
GCH II/III	enzyme variant SCO 6655 shares features of GCH II and GCH III	Streptomyces coelicolor

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.028	-	GTP	recombinant enzyme	Streptomyces coelicolor

Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.

Release 2023.1 (January 2023)