EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Reference |
---|
3.2.2.21 | -999 |
- |
specific activity 1100 pmol of 3-methyladenine released/mg of protein, 6.7 mg protein/4 ml |
646888 |
3.2.2.21 | -999 |
- |
the crude extract shows a specific activity of 80 IU/mg, the 125fold purified enzyme displays a specific activity of 10000 IU/mg, one international unit (IU) is defined as the activity, which cleaves 50% of 0.5 pmole of double stranded DNA oligo substrate containing a hypoxanthine lesion in 10 min at 37°C |
701054 |
3.2.2.21 | 0.00000008 |
- |
mutant Asn-152, expressed in Escherichia coli |
646887 |
3.2.2.21 | 0.000005 |
- |
mutant Asn-252, expressed in Escherichia coli |
646887 |
3.2.2.21 | 0.000007 |
- |
mutant Asn-255 and Asn-231, expressed in Escherichia coli |
646887 |
3.2.2.21 | 0.000009 |
- |
mutant Asn-259, expressed in Escherichia coli |
646887 |
3.2.2.21 | 0.00001 |
- |
mutant Asn-260, expressed in Escherichia coli |
646887 |
3.2.2.21 | 0.000013 |
- |
mutant Asn-112, expressed in Escherichia coli |
646887 |
3.2.2.21 | 0.000014 |
- |
wild-type Ndelta100Cdelta18, expressed in Escherichia coli |
646887 |
3.2.2.21 | 0.000018 |
- |
mutant Asn-310, expressed in Escherichia coli |
646887 |