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Literature summary for 3.2.2.21 extracted from

  • Birkeland, N.K.; Anensen, H.; Knaevelsrud, I.; Kristoffersen, W.; Bjoras, M.; Robb, F.T.; Klungland, A.; Bjelland, S.
    Methylpurine DNA glycosylase of the hyperthermophilic archaeon Archaeoglobus fulgidus (2002), Biochemistry, 41, 12697-12705.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
3-Methyladenine 5 mM, enzyme activity 110% Archaeoglobus fulgidus
3-Methylguanine 5 mM, enzyme activity 120% Archaeoglobus fulgidus

Cloned(Commentary)

Cloned (Comment) Organism
gene afalkA, AF2117 ORF cloned and overexpressed in Escherichia coli, gene function suppresses the alkylation sensitivity in Escherichia coli tag alkA double mutants Archaeoglobus fulgidus

Inhibitors

Inhibitors Comment Organism Structure
additional information resistant to product inhibition by free 3-methyladenine Archaeoglobus fulgidus
additional information resistant to product inhibition by free 3-methyladenine Escherichia coli
p-hydroxymercuribenzoate
-
Archaeoglobus fulgidus
ZnSO4 2 mM, enzyme activity 23% Archaeoglobus fulgidus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ 2 mM CaCl2, enzyme activity 103% Archaeoglobus fulgidus
Mg2+
-
Escherichia coli
Mg2+ 2 mM MgSO4, enzyme activity 112% Archaeoglobus fulgidus
Mn2+ 2 mM MnCl2, enzyme activity 102% Archaeoglobus fulgidus
Zn2+ 0.1 mM ZnSO4, enzyme activity 114% Archaeoglobus fulgidus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
34250
-
predicted from nucleotide sequence Archaeoglobus fulgidus
40000
-
recombinant protein, expressed in Escherichia coli, SDS-PAGE Archaeoglobus fulgidus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
alkylated DNA + H2O Escherichia coli
-
3-methyladenine + 7-methylguanine + O2-methylthymine + O2-methylcytosine + DNA
-
?
alkylated DNA + H2O Archaeoglobus fulgidus
-
3-methyladenine + 7-methylguanine + O2-methylthymine + O2-methylcytosine + DNA
-
?

Organism

Organism UniProt Comment Textmining
Archaeoglobus fulgidus
-
-
-
Escherichia coli
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Archaeoglobus fulgidus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
specific activity 1100 pmol of 3-methyladenine released/mg of protein, 6.7 mg protein/4 ml Archaeoglobus fulgidus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
alkylated DNA + H2O
-
Escherichia coli 3-methyladenine + 7-methylguanine + O2-methylthymine + O2-methylcytosine + DNA
-
?
alkylated DNA + H2O
-
Archaeoglobus fulgidus 3-methyladenine + 7-methylguanine + O2-methylthymine + O2-methylcytosine + DNA
-
?
alkylated DNA + H2O
-
Archaeoglobus fulgidus 3-methyladenine + 3-methylguanine + 7-methyladenine + 7-methylguanine + DNA
-
?
additional information
-
Escherichia coli ?
-
?
additional information excises alkylated bases only Archaeoglobus fulgidus ?
-
?

Synonyms

Synonyms Comment Organism
MPG
-
Archaeoglobus fulgidus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65 75
-
Archaeoglobus fulgidus

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 80
-
Archaeoglobus fulgidus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
45
-
activity reduced by 50% Archaeoglobus fulgidus
90
-
60% enzyme activity retained after incubation for 1 h Archaeoglobus fulgidus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5.2
-
-
Archaeoglobus fulgidus