Activating Compound | Comment | Organism | Structure |
---|---|---|---|
3-Methyladenine | 5 mM, enzyme activity 110% | Archaeoglobus fulgidus | |
3-Methylguanine | 5 mM, enzyme activity 120% | Archaeoglobus fulgidus |
Cloned (Comment) | Organism |
---|---|
gene afalkA, AF2117 ORF cloned and overexpressed in Escherichia coli, gene function suppresses the alkylation sensitivity in Escherichia coli tag alkA double mutants | Archaeoglobus fulgidus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | resistant to product inhibition by free 3-methyladenine | Archaeoglobus fulgidus | |
additional information | resistant to product inhibition by free 3-methyladenine | Escherichia coli | |
p-hydroxymercuribenzoate | - |
Archaeoglobus fulgidus | |
ZnSO4 | 2 mM, enzyme activity 23% | Archaeoglobus fulgidus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 2 mM CaCl2, enzyme activity 103% | Archaeoglobus fulgidus | |
Mg2+ | - |
Escherichia coli | |
Mg2+ | 2 mM MgSO4, enzyme activity 112% | Archaeoglobus fulgidus | |
Mn2+ | 2 mM MnCl2, enzyme activity 102% | Archaeoglobus fulgidus | |
Zn2+ | 0.1 mM ZnSO4, enzyme activity 114% | Archaeoglobus fulgidus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
34250 | - |
predicted from nucleotide sequence | Archaeoglobus fulgidus |
40000 | - |
recombinant protein, expressed in Escherichia coli, SDS-PAGE | Archaeoglobus fulgidus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
alkylated DNA + H2O | Escherichia coli | - |
3-methyladenine + 7-methylguanine + O2-methylthymine + O2-methylcytosine + DNA | - |
? | |
alkylated DNA + H2O | Archaeoglobus fulgidus | - |
3-methyladenine + 7-methylguanine + O2-methylthymine + O2-methylcytosine + DNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Archaeoglobus fulgidus | - |
- |
- |
Escherichia coli | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Archaeoglobus fulgidus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
specific activity 1100 pmol of 3-methyladenine released/mg of protein, 6.7 mg protein/4 ml | Archaeoglobus fulgidus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
alkylated DNA + H2O | - |
Escherichia coli | 3-methyladenine + 7-methylguanine + O2-methylthymine + O2-methylcytosine + DNA | - |
? | |
alkylated DNA + H2O | - |
Archaeoglobus fulgidus | 3-methyladenine + 7-methylguanine + O2-methylthymine + O2-methylcytosine + DNA | - |
? | |
alkylated DNA + H2O | - |
Archaeoglobus fulgidus | 3-methyladenine + 3-methylguanine + 7-methyladenine + 7-methylguanine + DNA | - |
? | |
additional information | - |
Escherichia coli | ? | - |
? | |
additional information | excises alkylated bases only | Archaeoglobus fulgidus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MPG | - |
Archaeoglobus fulgidus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | 75 | - |
Archaeoglobus fulgidus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 80 | - |
Archaeoglobus fulgidus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
activity reduced by 50% | Archaeoglobus fulgidus |
90 | - |
60% enzyme activity retained after incubation for 1 h | Archaeoglobus fulgidus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.2 | - |
- |
Archaeoglobus fulgidus |