EC Number   |
General Information |
Reference |
|---|
   3.4.22.70 | physiological function |
as a transpeptidase that covalently attaches various virulence factors to the cell surface, this enzyme plays a crucial role in the ability of bacteria to invade the host's tissues and to escape the immune response |
731478 |
| 3.4.22.70 | physiological function |
Bacillus anthracis uses the sortase A enzyme to anchor proteins to its cell wall envelope during vegetative growth |
709123 |
| 3.4.22.70 | more |
binding mechanism is a conformational selection followed by induced fit, and allosteric regulation of the enzyme, molecular dynamics simulations of the enzyme in apo state and when bound to an LPATG sorting signal, which adopts multiple metastable states, overview. The first and the second substrate binding sites are proposed to be located on opposing faces of the protein. The active sites of all sortases contain a conserved catalytic triad that consists of residues H120, C184, and R197. NMR SrtA structure covalently bound to a modified sorting signal, binding conformations, overview |
732881 |
| 3.4.22.70 | malfunction |
deficiency of srtA impairs the release of pro-inflammatory cytokines in Staphylococcus aureus-induced mastitis. A DEKTASrtA mutant strain attenuates the inflammatory reaction in the mammary tissue of mice compared with wild-type Staphylococcus aureus challenge. The enzyme mutant strain impairs the induction of pro-inflammatory cytokines such as tumor necrosis factor-alpha, interleukin-1beta, and interleukin-6, the mutant strain blocks the activation of nuclear factor kappaB and mitogen-activated protein kinases by attenuating the degradation and phosphorylation of signaling pathway molecules such as IkappaBalpha, p65 and p38. |
-, 731825 |
| 3.4.22.70 | malfunction |
deletion of srtA affects the attachment stage and results in a deficiency in biofilm production, deletion of srtA has no effect on cell viability and cell growth |
708707 |
| 3.4.22.70 | more |
during catalysis sortase A cleaves the conserved Leu-Pro-X-Thr-Gly sorting motif at the Thr residue under concomitant thioester formation at active site Cys184, mechanism, overview |
731485 |
| 3.4.22.70 | physiological function |
intraperitoneal immunization with recombinant SrtA confers to mice protection against Streptococcus pneumoniae intraperitoneal challenge |
708704 |
| 3.4.22.70 | physiological function |
sortase A catalyzes cell wall anchoring reaction of LPXTG surface proteins |
718379 |
| 3.4.22.70 | physiological function |
sortase A covalently attaches proteins to the bacterial cell wall by cleaving between threonine and glycine at an LPXTG recognition motif |
718278 |
| 3.4.22.70 | physiological function |
sortase A is a key virulence factor in the pathogenesis of Staphylococcus aureus-induced mastitis in mice, overview |
-, 731825 |
