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Literature summary for 3.4.22.70 extracted from

  • Weiner, E.M.; Robson, S.A.; Marohn, M.; Clubb, R.T.
    The sortase A enzyme that attaches proteins to the cell wall of Bacillus anthracis contains an unusual active site architecture (2010), J. Biol. Chem., 285, 23433-23443.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.4.22.70

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Bacillus anthracis

Protein Variants

Protein Variants Comment Organism
H126A inactive Bacillus anthracis

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.035
-
 o-aminobenzoyl-LPETG-2,4-dinitrophenyl sortase A DELTA64 (residues Asp65–Lys210), in 20 mM HEPES, pH 7.5 Bacillus anthracis
0.038
-
 o-aminobenzoyl-LPETG-2,4-dinitrophenyl sortase A DELTA56 (residues Asp-57-Lys210), in 20 mM HEPES, pH 7.5 Bacillus anthracis

Localization

Localization Comment Organism GeneOntology No. Textmining

Organism

Organism UniProt Comment Textmining
Bacillus anthracis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Talon His-affinity resin column chromatography and Sephacryl-100 gel filtration Bacillus anthracis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information SrtA recognizes the LPXTG-sorting signal through a lock-in-key mechanism Bacillus anthracis ?
-
 ?
o-aminobenzoyl-LPETG-2,4-dinitrophenyl + H2O
-
 Bacillus anthracis o-aminobenzoyl-LPETG + 2,4-dinitrophenol
-
 ?

Synonyms

Synonyms Comment Organism
SrtA
-
 Bacillus anthracis

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0000067
-
 o-aminobenzoyl-LPETG-2,4-dinitrophenyl sortase A DELTA56 (residues Asp-57-Lys210), in 20 mM HEPES, pH 7.5 Bacillus anthracis
0.0000083
-
 o-aminobenzoyl-LPETG-2,4-dinitrophenyl sortase A DELTA64 (residues Asp65–Lys210), in 20 mM HEPES, pH 7.5 Bacillus anthracis

General Information

General Information Comment Organism
physiological function Bacillus anthracis uses the sortase A enzyme to anchor proteins to its cell wall envelope during vegetative growth Bacillus anthracis

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.000183
-
 o-aminobenzoyl-LPETG-2,4-dinitrophenyl sortase A DELTA56 (residues Asp-57-Lys210), in 20 mM HEPES, pH 7.5 Bacillus anthracis
0.000233
-
 o-aminobenzoyl-LPETG-2,4-dinitrophenyl sortase A DELTA64 (residues Asp65–Lys210), in 20 mM HEPES, pH 7.5 Bacillus anthracis