EC Number   |
Reference  |
|---|
   4.1.99.13 | fusion protein is purified through a 20-ml amylose column and through heparin-agarose column |
680604 |
| 4.1.99.13 | by using glutathione-sepharose columns |
679920, 680605 |
| 4.1.99.13 | fusion protein is applied to amylose column. At this point the protein is above 90% pure. Further purification can be obtained by applying the eluted material to a 10 ml heparin-agarose column. Maltose-binding protein is removed by treatment with factor Xa protease |
681765 |
| 4.1.99.13 | by using a glutathione sepharose column and a Hi Trap Q column. Concentrated fusion protein is cleaved with thrombin from bovine plasma by incubation overnight at 4°C. For chromophore determination, the eluate from the glutathione sepharose column is purified through a Q sepharose column, omitting gel filtration procedure, and concentrated by ultrafiltration |
682048 |
| 4.1.99.13 | purified to near homogeneity from Drosophila embryonic cells by using NH4SO4 precipitation, SP sepharose column, UV DNA affinity beads, heparin sepharose column and Mono S column |
682049 |
| 4.1.99.13 | by fractionation of crude cell extracts with Heparin agarose and UV DNA affinity column chromatography |
682086 |
| 4.1.99.13 | NF-1 is isolated from nuclear extracts prepared from HeLa cell culture using a heparin column, an anion exchange Fractogel EMD TMAE-650 (S) column and a FPLC MonoS HR5/5 column |
682153 |
| 4.1.99.13 | by using a glutathione-sepharose column and UV-irradiated DNA affinity column, fusion protein is cleaved with thrombin |
682154 |
| 4.1.99.13 | (6-4) photoproduct DNA photolyase activity is detected in Crotalus atrox fibroblast. Activity is considerably enhanced when a UV-damaged DNA affinity column is used for purification. However, the activity is unstable and it is lost during purification or upon storage at -20° or -70°C for 2-3 months |
682264 |
| 4.1.99.13 | protein is isolated from whole cell extracts from Xenopus laevis, purified by using a sepharose column and a UV-damaged DNA affinity column |
682264 |
