Cloned (Comment) | Organism |
---|---|
due to insolubility problems (6-4) photolyases is overexpressed as a fusion protein in Escherichia coli. Plasmid pXZ1997, a derivative of pMal-c2 containing the Drosophila melanogaster phr(6-4) cDNA fused in frame to the malE gene encoding maltose-binding protein (MBP), is propagated in Escherichia coli strain UNC523 (phr::kan uvrA::Tn10) selecting for ampicillin resistance. Cells are cultured in 2 liter of LB to A600: 0.60.8. IPTG is added to 0.3 mM, and incubation continued for 6 h prior to harvesting the cells by centrifugation. | Drosophila melanogaster |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
110000 | - |
molecular weight of fusion protein, determined by SDS-PAGE | Drosophila melanogaster |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | - |
- |
- |
Purification (Comment) | Organism |
---|---|
fusion protein is applied to amylose column. At this point the protein is above 90% pure. Further purification can be obtained by applying the eluted material to a 10 ml heparin-agarose column. Maltose-binding protein is removed by treatment with factor Xa protease | Drosophila melanogaster |
Storage Stability | Organism |
---|---|
glycerol to a final concentration of 50% (v/v) is added to the photolyase, storage at -70°C | Drosophila melanogaster |
Synonyms | Comment | Organism |
---|---|---|
(6-4) photolyase | - |
Drosophila melanogaster |
phr (6-4) | - |
Drosophila melanogaster |