EC Number   |
Reference  |
|---|
   3.4.21.77 | one third of the purified protein is enzymatically inactive, due to carboxy-terminal cleavage of Lys145 |
647580 |
| 3.4.21.77 | - |
647582, 647587, 647588, 647594, 696922, 699819, 707359, 707618, 707727 |
| 3.4.21.77 | from LNCaP cell culture medium |
647590 |
| 3.4.21.77 | fast purification method with 90% of purity and 50% of recovery |
666798 |
| 3.4.21.77 | nickel-nitrilotriacetic acid-Sepharose column chromatography and benzamidine-Sepharose column chromatography |
680680 |
| 3.4.21.77 | by lysozyme, sonication, and centrifugation, His-tagged PSA solublized from inclusion bodies by urea and purified on nickel-chelate resin. GST-fused PSA solublized from inclusion bodies by sarcosyl and purified on glutathione agarose beads |
681362 |
| 3.4.21.77 | by HPLC |
681472 |
| 3.4.21.77 | by centrifugation, fractional precipitation by ammonium sulphate, ion-exchange chromatography and gel filtration, purified to homogeneity |
699063 |
| 3.4.21.77 | by thiophilic absorption chromatography |
699826 |
| 3.4.21.77 | from seminal plasma |
701037 |
