Application | Comment | Organism |
---|---|---|
medicine | purified active PSA has a potential use in prostate cancer diagnostics and/or therapeutics | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
proPSA and active PSA cDNA cleaved with EcoRI and HindIII and cloned into vector pET28(a)+, expressed in Escherichia coli strain BL21(DE3). Recombinant vector cleaved with EcoRI and XhoI, inserted into vector pGEX4T-1 and expressed in Escherichia coli strain BL21(DE3) | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54000 | - |
1 * 55000, SDS-PAGE, proPSA, 1 * 54000, SDS-PAGE, active PSA | Homo sapiens |
55000 | - |
1 * 55000, SDS-PAGE, proPSA, 1 * 54000, SDS-PAGE, active PSA | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P07288 | - |
- |
Purification (Comment) | Organism |
---|---|
by lysozyme, sonication, and centrifugation, His-tagged PSA solublized from inclusion bodies by urea and purified on nickel-chelate resin. GST-fused PSA solublized from inclusion bodies by sarcosyl and purified on glutathione agarose beads | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
LNCaP cell | - |
Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.004 | - |
purified recombinant active PSA | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
morpholinocarbonyl-His-Ser-Ser-Lys-Leu-Gln-7-amido-4-(trifluoromethyl)-coumarin + H2O | - |
Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 55000, SDS-PAGE, proPSA, 1 * 54000, SDS-PAGE, active PSA | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
prostate-specific antigen | - |
Homo sapiens |
PSA | - |
Homo sapiens |