EC Number   |
|---|
   2.7.11.20 | - |
| 2.7.11.20 | HiTrap Q column chromatography, and Superdex 75 gel filtration |
| 2.7.11.20 | Ni-NTA column chromatography and Superdex 75 gel filtration |
| 2.7.11.20 | Ni-NTA column chromatography, and Superdex 75 gel filtration |
| 2.7.11.20 | recombinant full-length human calmodulin (CaM), the predicted calmodulin-binding segment of human eEF-2K, residues 74-100 (eEF-2KCBD), and full-length eEF-2K |
| 2.7.11.20 | recombinant GST-tagged enzyme from Escherichia coli strain BL21(DE3) by affinity chromatography |
| 2.7.11.20 | recombinant GST-tagged wild-type and FLAG-tagged mutant enzymes from Escherichia coli |
| 2.7.11.20 | recombinant GST-tagged wild-type and mutant eEF2K enzymes from Escherichia coli strain Rosetta(DE3)pLysS |
| 2.7.11.20 | recombinant His-tagged enzyme from Escherichia coli strain BL21 Rosetta 2 by nickel affinity chromatography, ultrafiltration, cleavage of the tag with Sumo protease, and anion exchange chromatography, followed by gel filtration |
| 2.7.11.20 | with a nickel column by affinity chromatography |
