EC Number   |
Reference |
|---|
    2.7.1.50 | ammonium sulfate precipitation, Ni-NTA column chromatography, and gel filtration |
737878 |
| 2.7.1.50 | bifunctional enzyme with hydroxyethylthiazole kinase and thiamine-phosphate pyrophosphorylase activity |
639720 |
| 2.7.1.50 | heat treatment (363 K, 13 min), centrifugation, ion exchange chromatography, hydroxyapatite chromatography, gel filtration, |
701541 |
| 2.7.1.50 | recombinant enzyme from Escherichia coli strain BL21-CodonPlus(DE3)-RIL by gel filtration, anion exchange chromatography to over 95% purity |
721209 |
| 2.7.1.50 | recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, cleavage of the tag by TEV protease, and another step of nickel affinity chromatography |
721627 |
