Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | highest activity in the presence of Co2+ | Escherichia coli | |
Mg2+ | activates | Escherichia coli |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
28000 | - |
x * 28000, SDS-PAGE | Escherichia coli |
28100 | - |
- |
Escherichia coli |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | P76423 | - |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, Ni-NTA column chromatography, and gel filtration | Escherichia coli |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.9 | - |
with UTP as phosphate donor, in the presence of Mg2+, at pH 10.0 and 50°C | Escherichia coli |
1.1 | - |
with CTP as phosphate donor, in the presence of Mg2+, at pH 10.0 and 50°C | Escherichia coli |
1.4 | - |
with ATP as phosphate donor, in the presence of Mg2+, at pH 10.0 and 50°C | Escherichia coli |
3.2 | - |
with GTP as phosphate donor, in the presence of Mg2+, at pH 10.0 and 50°C | Escherichia coli |
3.9 | - |
with dCTP as phosphate donor, in the presence of Mg2+, at pH 10.0 and 50°C | Escherichia coli |
4 | - |
with dUTP as phosphate donor, in the presence of Mg2+, at pH 10.0 and 50°C | Escherichia coli |
4.3 | - |
with UTP as phosphate donor, in the presence of Co2+, at pH 10.0 and 50°C | Escherichia coli |
5.2 | - |
with CTP as phosphate donor, in the presence of Co2+, at pH 10.0 and 50°C | Escherichia coli |
8.9 | - |
with TTP as phosphate donor, in the presence of Mg2+, at pH 10.0 and 50°C | Escherichia coli |
9.2 | - |
with dATP as phosphate donor, in the presence of Mg2+, at pH 10.0 and 50°C | Escherichia coli |
11 | - |
with ATP as phosphate donor, in the presence of Co2+, at pH 10.0 and 50°C | Escherichia coli |
11 | - |
with dGTP as phosphate donor, in the presence of Co2+, at pH 10.0 and 50°C | Escherichia coli |
14 | - |
with dGTP as phosphate donor, in the presence of Mg2+, at pH 10.0 and 50°C | Escherichia coli |
14 | - |
with dUTP as phosphate donor, in the presence of Co2+, at pH 10.0 and 50°C | Escherichia coli |
15 | - |
with dCTP as phosphate donor, in the presence of Co2+, at pH 10.0 and 50°C | Escherichia coli |
24 | - |
with GTP as phosphate donor, in the presence of Co2+, at pH 10.0 and 50°C | Escherichia coli |
37 | - |
with dATP as phosphate donor, in the presence of Co2+, at pH 10.0 and 50°C | Escherichia coli |
45 | - |
with TTP as phosphate donor, in the presence of Co2+, at pH 10.0 and 50°C | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 4-methyl-5-(2-hydroxyethyl)thiazole | - |
Escherichia coli | ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole | - |
? | |
CTP + 4-methyl-5-(2-hydroxyethyl)thiazole | - |
Escherichia coli | CDP + 4-methyl-5-(2-phosphonooxyethyl)thiazole | - |
? | |
dATP + 4-methyl-5-(2-hydroxyethyl)thiazole | the enzyme shows a preference for dATP as a phosphate donor over ATP | Escherichia coli | dADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole | - |
? | |
dCTP + 4-methyl-5-(2-hydroxyethyl)thiazole | - |
Escherichia coli | dCDP + 4-methyl-5-(2-phosphonooxyethyl)thiazole | - |
? | |
dGTP + 4-methyl-5-(2-hydroxyethyl)thiazole | - |
Escherichia coli | dGDP + 4-methyl-5-(2-phosphonooxyethyl)thiazole | - |
? | |
dUTP + 4-methyl-5-(2-hydroxyethyl)thiazole | - |
Escherichia coli | dUDP + 4-methyl-5-(2-phosphonooxyethyl)thiazole | - |
? | |
GTP + 4-methyl-5-(2-hydroxyethyl)thiazole | - |
Escherichia coli | GDP + 4-methyl-5-(2-phosphonooxyethyl)thiazole | - |
? | |
TTP + 4-methyl-5-(2-hydroxyethyl)thiazole | - |
Escherichia coli | TDP + 4-methyl-5-(2-phosphonooxyethyl)thiazole | - |
? | |
UTP + 4-methyl-5-(2-hydroxyethyl)thiazole | - |
Escherichia coli | UDP + 4-methyl-5-(2-phosphonooxyethyl)thiazole | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 28000, SDS-PAGE | Escherichia coli |
? | x * 28100, calculated from amino acid sequence | Escherichia coli |
Synonyms | Comment | Organism |
---|---|---|
4-methyl-5-hydroxyethylthiazole kinase | - |
Escherichia coli |
ThiM | - |
Escherichia coli |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 60 | no decrease in the enzyme activity is detected at temperatures lower than 50°C, although about 60% of its original activity is lost at 60°C after 20 min | Escherichia coli |