2.3.1.214 | native Ss5MaT2 510fold by ammonium sulfate fractionation, a first anion exchange chromatographic step, hydrophobic interaction and ceramic hydroxyapatite chromatography, gel filtration, and a second, different anion exchange chromatograpic step to over 95% purity. Recombinant N-terminally His-tagged SsAT412 from Escherichia coli strain JM109 to homogeneity |
720699 |