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Literature summary for 2.3.1.214 extracted from
- Identification and characterization of a novel anthocyanin malonyltransferase from scarlet sage (Salvia splendens) flowers: an enzyme that is phylogenetically separated from other anthocyanin acyltransferases (2004), Plant J., 38, 994-1003.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| Ss5MaT2 or SsAT412, DNA and amino acid sequence determination and analysis, phylogenetic analysis, soluble N-terminally His-tagged SsAT412 expression in Escherichia coli strain JM109 | Salvia splendens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0069 | - |
malonyl-CoA | pH 8.5, temperature not specified in the publication | Salvia splendens |  |
| 0.0513 | - |
4'''-demalonylsalvianin | pH 8.5, temperature not specified in the publication | Salvia splendens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 43643 | - |
1 * 46000, SDS-PAGE, 1 * 43643, sequence calculation | Salvia splendens |
| 45000 | - |
gel filtration | Salvia splendens |
| 46000 | - |
1 * 46000, SDS-PAGE, 1 * 43643, sequence calculation | Salvia splendens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Salvia splendens | - |
scarlet sage | - |
| Salvia splendens | Q6TXD2 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| native Ss5MaT2 510fold by ammonium sulfate fractionation, a first anion exchange chromatographic step, hydrophobic interaction and ceramic hydroxyapatite chromatography, gel filtration, and a second, different anion exchange chromatograpic step to over 95% purity. Recombinant N-terminally His-tagged SsAT412 from Escherichia coli strain JM109 to homogeneity | Salvia splendens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| flower | recently opened, fully pigmented flowers | Salvia splendens | - |
| additional information | quantitative real-time PCR expression analysis in tissues, ovverview | Salvia splendens | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.144 | - |
purified native enzyme, pH 8.5, temperature not specified in the publication | Salvia splendens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| malonyl-CoA + 4'''-demalonylsalvianin | - |
Salvia splendens | CoA + salvianin | - |
? | |
| malonyl-CoA + monodemalonylsalvianin | Ss5MaT2 activity with monodemalonylsalvianin | Salvia splendens | CoA + salvianin | - |
? | |
| additional information | the enzyme does not show any 5MaT1 activity with bisdemalonylsalvianin as substrate | Salvia splendens | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 46000, SDS-PAGE, 1 * 43643, sequence calculation | Salvia splendens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| malonyl-CoA:anthocyanin 5-glucoside 4'''-O-malonyltransferase | - |
Salvia splendens |
| Ss5MaT2 | - |
Salvia splendens |
| SsAT412 | - |
Salvia splendens |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.55 | - |
malonyl-CoA | pH 8.5, temperature not specified in the publication | Salvia splendens | |
| 0.55 | - |
4'''-demalonylsalvianin | pH 8.5, temperature not specified in the publication | Salvia splendens | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8.5 | - |
- |
Salvia splendens |
General Information
| General Information | Comment | Organism |
|---|---|---|
| evolution | the enzyme belongs to the BAHD family of acyltrasferases, and phylogenetic analysis shows, that it is more related to the acetyl-CoA:benzylalcohol acetyltransferases than to the anthocyanin acyltransferases. Ss5MaT2 contains the BAHD conserved motifs 1 HXXXD and 3 DFGWG, but lacks motif 2 YFGNC | Salvia splendens |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 10.7 | - |
4'''-demalonylsalvianin | pH 8.5, temperature not specified in the publication | Salvia splendens | |
| 79.7 | - |
malonyl-CoA | pH 8.5, temperature not specified in the publication | Salvia splendens | |
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