EC Number |
Reference |
---|
2.3.1.54 | - |
14931, 14936, 14937, 487220, 487222, 487226, 487234, 487236, 487239 |
2.3.1.54 | partially |
487221 |
2.3.1.54 | recombinant enzyme |
487233, 487237 |
2.3.1.54 | native enzyme from strain BL21(DE3) by gel filtration and anion exchange chromatography, recombinant His6-tagged enzyme from strain BL21(DE3) by nickel affinity chromatography as single enzyme or as AdhE-Pfl |
671620 |
2.3.1.54 | recombinant His-tagged wild-type and mutant enzymes from strain BL21(DE3) by nickel affinity chromatography to homogeneity |
672694 |
2.3.1.54 | native enzyme at pH 6.4, by anion exchange and hydrophobic interaction chromatography, and gel filtration, to homogeneity |
675371 |
2.3.1.54 | gel filtration, Western Blot, heterologously expressed protein |
686477 |
2.3.1.54 | centrifugation, washing in 50 mM potassium phosphate buffer, pH 7.5, resuspension, French press disruption, centrifugation of crude extract and of resulting supernatant |
701826 |
2.3.1.54 | quaternary methylamine AP5-ion exchange column chromatography and phenyl Sepharose column chromatography |
757586 |