Cloned (Comment) | Organism |
---|---|
overexpression of the His6-tagged enzyme as single enzyme or as AdhE-Pfl in strain BL21(DE3) | Escherichia coli |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | rapid, reversible inactivation, deactivation is a non-destructive transfer of an H atom equivalent to quench the glycyl radical | Escherichia coli | |
DTT | reversible inactivation, deactivation is a non-destructive transfer of an H atom equivalent to quench the glycyl radical | Escherichia coli | |
additional information | the putative pyruvate formate-lyase deactivase, as activity of AdhE together with an alcohol dehydrogenase and an acetaldehyde-CoA dehydrogenase activities, is not active on the enzyme PFL, it has no PFL deactivating activity | Escherichia coli |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
CoA + pyruvate | Escherichia coli | - |
acetyl-CoA + formate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Escherichia coli | - |
gene pfl | - |
Purification (Comment) | Organism |
---|---|
native enzyme from strain BL21(DE3) by gel filtration and anion exchange chromatography, recombinant His6-tagged enzyme from strain BL21(DE3) by nickel affinity chromatography as single enzyme or as AdhE-Pfl | Escherichia coli |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
acetyl-CoA + formate = CoA + pyruvate | the reaction mechanism proceeds via generation of a catalytically essential glycyl radical at Gly734 | Escherichia coli |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
CoA + pyruvate | - |
Escherichia coli | acetyl-CoA + formate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PFL | - |
Escherichia coli |
pyruvate formate-lyase | - |
Escherichia coli |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
assay at | Escherichia coli |