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EC Number	Application	Commentary	Reference
6.2.1.30	analysis	determination of of microbody-borne enzymes by analysis of putative microbody targeting singals and a proteomics based inventory of Penicillium chrysogenum microbody matrix proteins using nano-LC-MS/MS analysis	703815
6.2.1.30	synthesis	engineering of Hansenula polymorpha for secretion of penicillin. Introduction of the Penicilliumchrysogenum gene encoding the non-ribosomal peptide synthetase delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase in Hansenula polymorpha, results in the production of active enzyme, when co-expressed with the Bacillus subtilis sfp gene encoding a phosphopantetheinyl transferase that activates ACVS. Co-expression with the Penicillium chrysogenum genes encoding the cytosolic enzyme isopenicillin N synthase as well as the two peroxisomal enzymes isopenicillin N acyl transferase and phenylacetyl CoA ligase results in production of biologically active penicillin, which is efficiently secreted. The amount of secreted penicillin is approximately 1 mg/l. Penicillin production is decreased over 2fold in a yeast strain lacking peroxisomes	706449
6.2.1.30	synthesis	the expression of the heterologous enzyme phenylacetyl-CoA ligase, involved in catabolism of a penicillin precursor, is a useful strategy for improving the biosynthetic machinery of this fungus	648920

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Release 2023.1 (January 2023)