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Literature summary for 6.2.1.30 extracted from
- An engineered yeast efficiently secreting penicillin (2009), PLoS ONE, 4, e8317.
Application
| Application | Comment | Organism |
|---|---|---|
| synthesis | engineering of Hansenula polymorpha for secretion of penicillin. Introduction of the Penicilliumchrysogenum gene encoding the non-ribosomal peptide synthetase delta-(L-alpha-aminoadipyl)-L-cysteinyl-D-valine synthetase in Hansenula polymorpha, results in the production of active enzyme, when co-expressed with the Bacillus subtilis sfp gene encoding a phosphopantetheinyl transferase that activates ACVS. Co-expression with the Penicillium chrysogenum genes encoding the cytosolic enzyme isopenicillin N synthase as well as the two peroxisomal enzymes isopenicillin N acyl transferase and phenylacetyl CoA ligase results in production of biologically active penicillin, which is efficiently secreted. The amount of secreted penicillin is approximately 1 mg/l. Penicillin production is decreased over 2fold in a yeast strain lacking peroxisomes | Penicillium chrysogenum |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Penicillium chrysogenum |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Penicillium chrysogenum | - |
- |
- |
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Release 2023.1 (January 2023)
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