EC Number   |
Application |
Reference |
|---|
   1.13.12.6 | molecular biology |
construction of a cold-induced expression vector (pCold-ZZ-VL vector) in Escherichia coli cells that results in soluble bioluminescent fusion enzyme with binding ability to monoclonal antibodies, however, the Cypridina luciferase fusion enzyme is soluble but not bioluminescent (in contrast to other luciferases fused to the ZZ-domain of Staphylococcuss aureus protein A) |
696008 |
| 1.13.12.6 | diagnostics |
Cypridina luciferase is used as bioluminescence reporter enzyme in yeast, bacterial, and mammalian cell-based assays, enzyme activity is measured with a luminometer by addition of native or synthetic luciferin, also known as Vargulin, 0.5 microM in 10 mM Tris-HCl, pH 7.4 |
700006 |
| 1.13.12.6 | analysis |
Cypridina noctiluca luciferase is utilized for biochemical and molecular biological applications, including bioluminescent enzyme immunoassays, far-red luminescence imaging, and high-throughput reporter assays |
746376 |
| 1.13.12.6 | molecular biology |
Cypridina noctiluca luciferase is utilized for biochemical and molecular biological applications, including bioluminescent enzyme immunoassays, far-red luminescence imaging, and high-throughput reporter assays |
746376 |
| 1.13.12.6 | analysis |
dual reporter assay using one luciferase for monitoring gene expression and a second as an internal control, based on secreted luciferases from Cypridina noctiluca and Gaussia princeps that do not require lysis or special equipment. The assay can be carried out sequentially, the activities are high and can be measured in a small volume and a simple procedure. Development of a one-tube reporter assay for the two enzymes |
685701 |
| 1.13.12.6 | diagnostics |
far-red luminescence imaging technology to visualize tumor specific antigens on cell surfaces in vitro (human hepato-cellular carcinoma cell line Huh-7) and in living bodies (mice xenografted with human Delta-like protein-positive Huh-7 cells), based on a far-red fluorescent indocyanine derivative conjugated to biotinylated Cypridina luciferase linked to an anti-human Delta-like protein (Dlk-1) monoclonal antibody (embryonic antigens expressed on the surface of many cancer cells) via biotin-avidin interaction |
700998 |
| 1.13.12.6 | analysis |
intramolecular bioluminescence resonance energy transfer system consisting of a fusion protein of the enzyme and enhanced yellow fluorescent protein for investigating peptide processing in living cells |
654184 |
| 1.13.12.6 | diagnostics |
labeling of prostaglandin E2 with the bioluminescent enzyme Cypridina luciferase linked to monoclonal anti-prostaglandin E2 antibody as enzyme-linked immunosorbent assay (ELISA) system, detection of 7.8-500 pg/ml prostaglandin E2 |
699954 |
| 1.13.12.6 | molecular biology |
luciferases can be used as light-emissive reporters of mechanoenzymatic reaction. The light emitted by a bioluminescent reaction can be used to directly monitor the progress of a mechanoenzymatic reaction without sampling |
763892 |
| 1.13.12.6 | molecular biology |
Renilla and Cypridina luciferases should be more appropriate tools for applications requiring the detection of small amounts of substrate as in small molecule detection assays because RLuc and CLuc respond to their luciferin concentration in a linear non-cooperative manner |
728578 |
