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(S)-Cypridina luciferin I + O2
oxidized S-Cypridina luciferin I + CO2 + hv
-
-
-
-
?
1-{3-[6-(1H-indol-3-yl)-2-(1-methylpropyl)-3-oxo-3,7-dihydroimidazo[1,2-a]pyrazin-8-yl]propyl}guanidine + O2
N-[3-(3-carbamimidamidopropyl)-5-(1H-indol-3-yl)-1,4-dihydropyrazin-2-yl]-2-methylbutanamide + CO2 + hn
-
-
-
?
Cypridina luciferin + O2
?
-
fusion enzyme + 10 mM luciferin (dissolved in ethanol in 30 mM Tris-HCl (pH 7.6) with 10 mM EDTA) with ATP (250 mM), CoA (250 microM), and MgCl2 (5 mM) in 100 mM Tris-HCl, pH 7.8
Cypridina luciferase fusion enzyme expressed in Escherichia coli is soluble but does not exhibit bioluminescence
-
?
Cypridina luciferin + O2
oxidized Cypridina luciferin + CO2 + hv
DL-Cypridina luciferin + O2
oxidized DL-Cypridina luciferin + CO2 + hv
-
-
-
-
?
luciferin + O2
oxidized luciferin + CO2 + hnu
-
-
-
-
r
Vargula hilgendorfii luciferin + O2
oxidized Vargula hilgendorfii luciferin + CO2 + hv
native or recombinant substrate protein
-
-
?
additional information
?
-
Cypridina sp.
-
evaluation of a method of measuring activity of Cypridina luciferase under conditions which minimize complicating with a relatively simple photoelectric light integrator, detailed overview
-
-
?
Cypridina luciferin + O2
oxidized Cypridina luciferin + CO2 + hv
-
-
-
-
?
Cypridina luciferin + O2
oxidized Cypridina luciferin + CO2 + hv
-
-
-
?
Cypridina luciferin + O2
oxidized Cypridina luciferin + CO2 + hv
-
-
-
-
?
Cypridina luciferin + O2
oxidized Cypridina luciferin + CO2 + hv
-
-
bioluminescence
-
?
Cypridina luciferin + O2
oxidized Cypridina luciferin + CO2 + hv
-
-
bioluminescence, maximum wavelength emission at 465 nm
-
?
Cypridina luciferin + O2
oxidized Cypridina luciferin + CO2 + hv
-
-
conjugated enzyme exhibits bimodal bioluminescence spectrum at 460 nm and 675 nm, exhibits higher light intensity in mouse blood than unconjugated enzyme, far-red emission peak dominates in blood while the 460 nm peak dominates in buffers
-
?
Cypridina luciferin + O2
oxidized Cypridina luciferin + CO2 + hv
-
luciferase reaction catalyzed by CLuc is first order with respect to their luciferin. The rate of light generation by CLuc is linear with respect to the concentration of its substrate, Cypridina luciferin
-
-
?
Cypridina luciferin + O2
oxidized Cypridina luciferin + CO2 + hv
Cypridina sp.
-
-
-
?
Cypridina luciferin + O2
oxidized Cypridina luciferin + CO2 + hv
Cypridina sp.
-
-
-
-
?
Cypridina luciferin + O2
oxidized Cypridina luciferin + CO2 + hv
-
-
-
?
Cypridina luciferin + O2
oxidized Cypridina luciferin + CO2 + hv
-
-
-
?
Cypridina luciferin + O2
oxidized Cypridina luciferin + CO2 + hv
-
-
-
?
Cypridina luciferin + O2
oxidized Cypridina luciferin + CO2 + hv
-
-
-
?
Cypridina luciferin + O2
oxidized Cypridina luciferin + CO2 + hv
-
-
-
?
Cypridina luciferin + O2
oxidized Cypridina luciferin + CO2 + hv
-
-
-
?
Cypridina luciferin + O2
oxidized Cypridina luciferin + CO2 + hv
-
-
-
-
?
Cypridina luciferin + O2
oxidized Cypridina luciferin + CO2 + hv
-
-
?
Cypridina luciferin + O2
oxidized Cypridina luciferin + CO2 + hv
-
-
-
?
Cypridina luciferin + O2
oxidized Cypridina luciferin + CO2 + hv
-
very low bioluminescence rate with biluciferyl
-
?
Cypridina luciferin + O2
oxidized Cypridina luciferin + CO2 + hv
-
Cypridina luciferin is [3-[3,7-dihydro-6-(1H-indol-3-yl)-2-[(S)-1-methyl-6-propyl]-3-oxoimidazo[1,2-a]pyrazin-8-yl]propyl]guanidine, equimolar complex of oxyluciferin with luciferase in an excited state is the light-emitter of Cypridina bioluminescence
-
?
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N182D
site-directed mutagenesis, mutation of a residue involved in a phoshorylation site, the mutant enzyme behaves similar compared to the wild-type
N182D/N404D
site-directed mutagenesis, mutation of a residue involved in a phoshorylation site, the mutant shows over slightly increased protein accumulation compared to the wild-type
N182D/S406A
site-directed mutagenesis, mutation of a residue involved in a phoshorylation site, the mutant shows over slightly increased protein accumulation compared to the wild-type
N404D
site-directed mutagenesis, mutation of a residue involved in a phoshorylation site, the mutant enzyme behaves similar to the wild-type
S406A
site-directed mutagenesis, mutation of a residue involved in a phoshorylation site, the mutant shows increased protein accumulation compared to the wild-type
T184A
site-directed mutagenesis, mutation of a residue involved in a phoshorylation site, the mutant shows increased protein accumulation compared to the wild-type
T184A/S406A
site-directed mutagenesis, mutation of a residue involved in a phoshorylation site, the mutant shows over slightly increased protein accumulation compared to the wild-type
T184A/N404D
site-directed mutagenesis, mutation of a residue involved in a phoshorylation site, the mutant shows over 2.5fold increased protein accumulation compared to the wild-type
T184A/N404D
both N-glycosylation sites are mutated and lost
additional information
-
biotynilation of the enzyme by attachment of an Avi-tag of a 16-residue peptide to the C-terminus of the luciferase, conjugation to the indocyanine derivative HiLyte Fluor 647 hydrazine via the glycol-chains of the enzyme
additional information
-
conjugation of prostaglandin E2 to luciferase by producing the N-hydroxysuccinimidyl ester of prostaglandin E2 by reaction of N-hydroxysuccinimde and 1-ethyl-3-(dimethylaminopropyl)carbodiimide hydrochloride and conjugation of the ester to luciferase (ratio 18/1) in 0.1 M potassium phosphate buffer, pH 7.2, with 0.15 M NaCl for 13 h on ice
additional information
-
construction of a cold-induced expression vector (pCold-ZZ-VL vector) in Escherichia coli cells that consists of a histidine tag sequence for nickel chelate affinity purification, IgG-binding domain of Staphylococcuss aureus protein A (ZZ-domain) and multiple cloning sites, fusing Cypridina luciferase to the ZZ-domain results in expression by Escherichia coli of a soluble cytoplasm enzyme but it is not bioluminescent (in contrast to other luciferases fused to the ZZ-domain)
additional information
-
establishment and validation of a yeast reporter assay, for detection of endocrine disruptors and analysis of protein-protein interactions by the yeast two-hybrid system, using a secretory luciferase, Cypridina noctiluca luciferase CLuc, as an alternative to the conventional beta-galactosidase, the CLuc reporter assay in yeast is more sensitive and convenient than the conventional assay, determination of the transcriptional activity of hundreds of yeast promoter fragments, overview
additional information
concept of tumor monitoring using dual luciferases, construction of the expression vector, and evaluation of tumor monitoring systems using dual luciferases from Cypridina noctiluca and firefly Photinus pyralis, overview. The enzymes are expressed in human breast cancercells MDA-MB-231, followed by inoculatin of the MDA-MB-231/FIC cell suspension subcutaneously into the back of 6-week-old male nude mice lacking T-cell function (BALB/cAJcl-nu/nu). The expressed CLuc is secreted into the blood from the cells and circulates in the living body. The blood containing CLuc can be drawn by using glass micro-hematocrit capillary tubes or a syringe
additional information
for large scale enzyme production, a simple production procedure is established using plant cell cultures. The plant cell culture tobacco BY-2 efficiently secretes luciferase, which is easily purified using a simple one-step ion-exchange chromatography method. The production yield is 20-30 mg of luciferase per liter of culture medium. The method offers a cost-effective production for Cypridina luciferase
additional information
-
for large scale enzyme production, a simple production procedure is established using plant cell cultures. The plant cell culture tobacco BY-2 efficiently secretes luciferase, which is easily purified using a simple one-step ion-exchange chromatography method. The production yield is 20-30 mg of luciferase per liter of culture medium. The method offers a cost-effective production for Cypridina luciferase
additional information
wild-type and mutant enzymes show similar secretion efficiencies, and thermostabilities at 25-37°C, overview
additional information
-
wild-type and mutant enzymes show similar secretion efficiencies, and thermostabilities at 25-37°C, overview
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biotechnology
use of enzyme as a potent secreted reporter
analysis
-
intramolecular bioluminescence resonance energy transfer system consisting of a fusion protein of the enzyme and enhanced yellow fluorescent protein for investigating peptide processing in living cells
analysis
-
dual reporter assay using one luciferase for monitoring gene expression and a second as an internal control, based on secreted luciferases from Cypridina noctiluca and Gaussia princeps that do not require lysis or special equipment. The assay can be carried out sequentially, the activities are high and can be measured in a small volume and a simple procedure. Development of a one-tube reporter assay for the two enzymes
analysis
-
use of enzyme as a reporter in a screening system of Saccharomyces cerevisiae to improve secretion efficiency in yeast
analysis
Cypridina noctiluca luciferase is utilized for biochemical and molecular biological applications, including bioluminescent enzyme immunoassays, far-red luminescence imaging, and high-throughput reporter assays
analysis
the secreted Cypridina luciferase (CLuc) is used as an ex vivo indicator to continuously monitor tumor progression. On the other hand, the non-secreted firefly luciferase is used as an in vivo indicator to analyze the spatial distribution of the tumor at suitable time points indicated by CLuc. Tumor monitoring systems using dual luciferases are available, allowing long-term bioluminescence imaging under minimal stress for the experimental animals, e.g. BALB/cAJcl-nu/nu mice. Continuous non-invasive measurement of CLuc activity for tumor growth allows us to determine a suitable time to assess tumor spatial distribution and growth, thereby, reducing the animal stress and the experimental cost
analysis
the thermostable enzyme can be used for various research applications, including in vivo imaging and high throughput reporter assays
diagnostics
-
Cypridina luciferase is used as bioluminescence reporter enzyme in yeast, bacterial, and mammalian cell-based assays, enzyme activity is measured with a luminometer by addition of native or synthetic luciferin, also known as Vargulin, 0.5 microM in 10 mM Tris-HCl, pH 7.4
diagnostics
-
far-red luminescence imaging technology to visualize tumor specific antigens on cell surfaces in vitro (human hepato-cellular carcinoma cell line Huh-7) and in living bodies (mice xenografted with human Delta-like protein-positive Huh-7 cells), based on a far-red fluorescent indocyanine derivative conjugated to biotinylated Cypridina luciferase linked to an anti-human Delta-like protein (Dlk-1) monoclonal antibody (embryonic antigens expressed on the surface of many cancer cells) via biotin-avidin interaction
diagnostics
-
labeling of prostaglandin E2 with the bioluminescent enzyme Cypridina luciferase linked to monoclonal anti-prostaglandin E2 antibody as enzyme-linked immunosorbent assay (ELISA) system, detection of 7.8-500 pg/ml prostaglandin E2
molecular biology
-
construction of a cold-induced expression vector (pCold-ZZ-VL vector) in Escherichia coli cells that results in soluble bioluminescent fusion enzyme with binding ability to monoclonal antibodies, however, the Cypridina luciferase fusion enzyme is soluble but not bioluminescent (in contrast to other luciferases fused to the ZZ-domain of Staphylococcuss aureus protein A)
molecular biology
-
Renilla and Cypridina luciferases should be more appropriate tools for applications requiring the detection of small amounts of substrate as in small molecule detection assays because RLuc and CLuc respond to their luciferin concentration in a linear non-cooperative manner
molecular biology
Cypridina noctiluca luciferase is utilized for biochemical and molecular biological applications, including bioluminescent enzyme immunoassays, far-red luminescence imaging, and high-throughput reporter assays
molecular biology
luciferases can be used as light-emissive reporters of mechanoenzymatic reaction. The light emitted by a bioluminescent reaction can be used to directly monitor the progress of a mechanoenzymatic reaction without sampling
molecular biology
the thermostable enzyme can be used for various research applications, including in vivo imaging and high throughput reporter assays
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Tsuji, F.I.
Cypridina luciferin and luciferase
Methods Enzymol.
57
364-372
1978
Vargula hilgendorfii
-
brenda
Tsuji, F.I.; Lynch, R.V.; Stevens, C.L.
Some properties of luciferase from the bioluminescent crustacean, Cypridina hilgendorfii
Biochemistry
13
5204-5209
1974
Vargula hilgendorfii
brenda
Cormier, M.J.; Crane, J.M.; Nakano, Y.
Evidence for the identity of the luminescent systems of Porichthys porosissimus (fish) and Cypridina hilgendorfii (crustacean)
Biochem. Biophys. Res. Commun.
29
747-752
1967
Porichthys porosissimus
brenda
Stone, H.
The enzyme catalyzed oxidation of Cypridina luciferin
Biochem. Biophys. Res. Commun.
31
386-391
1968
Cypridina sp.
brenda
Tsuji, F.I.; DeLuca, M.; Boyer, P.D.; Endo, S.; Akutagawa, M.
Mechanism of the enzyme-catalyzed oxidation of Cypridina and firefly luciferins studied by means of 17O2 and H218O1
Biochem. Biophys. Res. Commun.
74
606-613
1977
Vargula hilgendorfii
brenda
Toya, Y.; Nakatsuka, S.; Goto, T.
Structure of cypridina biluceferyl, a dimer of cypriidna luciferyl radical having bioluminescent activity
Tetrahedron Lett.
26
239-242
1985
Vargula hilgendorfii
-
brenda
Shimomura, O.; Johnson, F.H.; Masugi, T.
Cypridina bioluminescence: light-emitting oxyluciferin-luciferase complex
Science
13
1299-1300
1969
Vargula hilgendorfii
-
brenda
Maeda, Y.; Ueda, H.; Kazami, J.; Kawano, G.; Suzuki, E.; Nagamune, T.
Engineering of functional chimeric protein G-Vargula luciferase
Anal. Biochem.
249
147-152
1997
Vargula hilgendorfii
brenda
Inouye, S.
Fusions to imidazopyrazinone-type luciferases and aequorin as reporters
Methods Enzymol.
326
165-174
2000
Vargula hilgendorfii (P17554)
brenda
Otsuji, T.; Okuda-Ashitaka, E.; Kojima, S.; Akiyama, H.; Ito, S.; Ohmiya, Y.
Monitoring for dynamic biological processing by intramolecular bioluminescence resonance energy transfer system using secreted luciferase
Anal. Biochem.
329
230-237
2004
Vargula hilgendorfii
brenda
Nakajima, Y.; Kobayashi, K.; Yamagishi, K.; Enomoto, T.; Ohmiya, Y.
cDNA cloning and characterization of a secreted luciferase from the luminous Japanese ostracod, Cypridina noctiluca
Biosci. Biotechnol. Biochem.
68
565-570
2004
Cypridina noctiluca (Q75R40)
brenda
Yamagishi, K.; Enomoto, T.; Ohmiya, Y.
Perfusion-culture-based secreted bioluminescence reporter assay in living cells
Anal. Biochem.
354
15-21
2006
Cypridina noctiluca
brenda
Wu, C.; Kawasaki, K.; Ogawa, Y.; Yoshida, Y.; Ohgiya, S.; Ohmiya, Y.
Preparation of biotinylated Cypridina luciferase and its use in bioluminescent enzyme immunoassay
Anal. Chem.
79
1634-1638
2007
Cypridina noctiluca
brenda
Wu, C.; Kawasaki, K.; Ohgiya, S.; Ohmiya, Y.
Syntheses and evaluation of the bioluminescent activity of (S)-Cypridina luciferin and its analogs
Tetrahedron Lett.
47
753-756
2006
Vargula hilgendorfii
-
brenda
Kanjou, N.; Nagao, A.; Ohmiya, Y.; Ohgiya, S.
Yeast mutant with efficient secretion identified by a novel secretory reporter, Cluc
Biochem. Biophys. Res. Commun.
358
429-434
2007
Cypridina noctiluca
brenda
Wu, C.; Suzuki-Ogoh, C.; Ohmiya, Y.
Dual-reporter assay using two secreted luciferase genes
Biotechniques
42
290,292
2007
Cypridina noctiluca
brenda
Inouye, S.; Sahara, Y.
Soluble protein expression in E. coli cells using IgG-binding domain of protein A as a solubilizing partner in the cold induced system
Biochem. Biophys. Res. Commun.
376
448-453
2008
Cypridina noctiluca
brenda
Wu, C.; Irie, S.; Yamamoto, S.; Ohmiya, Y.
A bioluminescent enzyme immunoassay for prostaglandin E(2) using Cypridina luciferase
Luminescence
24
131-133
2009
Cypridina noctiluca
brenda
Michelini, E.; Cevenini, L.; Mezzanotte, L.; Roda, A.
Luminescent probes and visualization of bioluminescence
Methods Mol. Biol.
574
1-13
2009
Cypridina noctiluca
brenda
Wu, C.; Mino, K.; Akimoto, H.; Kawabata, M.; Nakamura, K.; Ozaki, M.; Ohmiya, Y.
In vivo far-red luminescence imaging of a biomarker based on BRET from Cypridina bioluminescence to an organic dye
Proc. Natl. Acad. Sci. USA
106
15599-15603
2009
Cypridina noctiluca
brenda
Tochigi, Y.; Sato, N.; Sahara, T.; Wu, C.; Saito, S.; Irie, T.; Fujibuchi, W.; Goda, T.; Yamaji, R.; Ogawa, M.; Ohmiya, Y.; Ohgiya, S.
Sensitive and convenient yeast reporter assay for high-throughput analysis by using a secretory luciferase from Cypridina noctiluca
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82
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2010
Cypridina noctiluca
brenda
Tzertzinis, G.; Schildkraut, E.; Schildkraut, I.
Substrate cooperativity in marine luciferases
PLoS ONE
7
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2012
Cypridina noctiluca
brenda
Morita, N.; Haga, S.; Ohmiya, Y.; Ozaki, M.
Long-term ex vivo and in vivo monitoring of tumor progression by using dual luciferases
Anal. Biochem.
497
24-26
2016
Cypridina noctiluca (Q75R40)
brenda
Chase, A.
Activity and inhibition of Cypridina luciferase quantitative measurement, analysis of inhibition by urea, and some effects of sodium and potassium ions
Biolumin. Progr.
2015
115-136
2015
Cypridina sp.
-
brenda
Tsuji, F.; Haneda, Y.
Chemistry of the luciferases of Cypridina hilgendorfii and Apogon ellioti
Biolumin. Progr.
2015
137-149
2015
Vargula hilgendorfii, Apogon ellioti
-
brenda
Yasuno, R.; Mitani, Y.; Ohmiya, Y.
Effects of N-glycosylation deletions on Cypridina luciferase activity
Photochem. Photobiol.
94
338-342
2018
Cypridina noctiluca (Q75R40), Cypridina noctiluca
brenda
Mitani, Y.; Oshima, Y.; Mitsuda, N.; Tomioka, A.; Sukegawa, M.; Fujita, M.; Kaji, H.; Ohmiya, Y.
Efficient production of glycosylated Cypridina luciferase using plant cells
Protein Expr. Purif.
133
102-109
2017
Cypridina noctiluca (Q75R40), Cypridina noctiluca
brenda
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Mechanically assisted bioluminescence with natural luciferase
Angew. Chem. Int. Ed. Engl.
59
16485-16489
2020
Vargula hilgendorfii (P17554)
brenda
Mitani, Y.; Yasuno, R.; Kihira, K.; Chung, K.; Mitsuda, N.; Kanie, S.; Tomioka, A.; Kaji, H.; Ohmiya, Y.
Host-dependent producibility of recombinant Cypridina noctiluca luciferase with glycosylation defects
Front. Bioeng. Biotechnol.
10
774786
2022
Cypridina noctiluca (Q75R40)
brenda
Kanie, S.; Komatsu, M.; Mitani, Y.
Luminescence of Cypridina luciferin in the presence of human plasma alpha 1-acid glycoprotein
Int. J. Mol. Sci.
21
7516
2020
Cypridina noctiluca (Q75R40)
brenda
Bessho-Uehara, M.; Yamamoto, N.; Shigenobu, S.; Mori, H.; Kuwata, K.; Oba, Y.
Kleptoprotein bioluminescence Parapriacanthus fish obtain luciferase from ostracod prey
Sci. Adv.
6
eaax4942
2020
Vargula hilgendorfii (P17554), Cypridina noctiluca (Q75R40)
brenda