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EC Number
Organic Solvent
Commentary
Reference
3.4.22.3
Ethanol
12% (v/v), 4°C, pH 3.2, 530 h, about 35% loss of activity
752461
3.4.22.3
guanidine-HCl
ficin loses its complete structure in presence of 4 M guanidine-HCl at pH 7.0, ficin loses 50% activity at around 1 M guanidine-HCl, and is complete inactivated at 3 M
718344
3.4.22.3
urea
the enzyme activity decreases significantly at low concentration of urea before unfolding of the enzyme molecule (about 50% residual activity at 2 M, about 20% residual activity at 4 M, no activity at 6 M)
718344
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