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Literature summary for 3.4.22.3 extracted from
- Comparative stability of ficin and papain in acidic conditions and the presence of ethanol (2019), Amino Acids, 51, 829-838 .
Application
| Application | Comment | Organism |
|---|---|---|
| food industry | prolonged stability of ficin at low pH values in comparison to papain can be of importance for industrial processes that run in low pH conditions such as chill haze prevention during winemaking which prompted us to check long term stability of ficin and papain at low pH and in the presence of ethanol | Ficus carica |
Organic Solvent Stability
| Organic Solvent | Comment | Organism |
|---|---|---|
| Ethanol | 12% (v/v), 4°C, pH 3.2, 530 h, about 35% loss of activity | Ficus carica |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ficus carica | - |
mixture of ficin isoforms | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| latex | mixture of ficin isoforms | Ficus carica | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, pH 3.2, 530 h, about 35% loss of activity | Ficus carica |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| casein + H2O | - |
Ficus carica | ? | - |
? |  |
| Nalpha-benzoyl-DL-arginine-4-nitroanilide + H2O | - |
Ficus carica | Nalpha-benzoyl-DL-arginine + 4-nitroaniline | - |
? | |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
pH 3.2, 30 min, 5% loss of activity | Ficus carica |
| 45 | - |
pH 3.2: inactivation constant: 0.0068/min | Ficus carica |
| 46 | - |
pH 3.2, Tm-value: 45.64°C | Ficus carica |
| 50 | - |
pH 3.2: inactivation constant: 0.02/min | Ficus carica |
| 51 | - |
pH 3.2, Tm-value: 51.34°C | Ficus carica |
| 55 | - |
pH 3.2: inactivation constant: 0.08/min | Ficus carica |
| 60 | - |
pH 3.2: inactivation constant: 0.234/min | Ficus carica |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 8 | - |
assay at | Ficus carica |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 10 | the enzyme (mixture of ficin isoform) retains more than 80% of initial activity after 30 min incubation at pH 3.0-10.0 | Ficus carica |
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Release 2023.1 (January 2023)
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