EC Number   |
Natural Substrates |
|---|
   3.4.22.B19 | Cdc42 + H2O |
- |
| 3.4.22.B19 | IkappaBalpha-ubiquitin + H2O |
- |
| 3.4.22.B19 | more |
the virulence factor YopJ cleaves reversibly post-translational modification in form of ubiquitin or a ubiquitin-like protein. It inhibits the host immune response and induces apoptosis by blocking multiple signaling pathways, including the MAPK and NfkappaB pathways in the infected cell |
| 3.4.22.B19 | more |
substrates are highly conserved ubiquitin-like molecules, which are covalently added to numerous regulatory proteins. YopJ protease exerts its pathogenic effect on cells by disrupting this posttranslational modification |
| 3.4.22.B19 | more |
interaction of Yersinia pestis with macrophages, limitations in YopJ-dependent apoptosis |
| 3.4.22.B19 | more |
mice infected with wild-type strain of Yersinia pseudotuberculosis show the highest TNF-alpha production on the 21st day after infection in the culture supernatant of cells incubated with LPS. During the infection with mutant strains unable to secrete YopJ, there is no TNF-alpha production on both the 7th and 28th days after infection, but the production is increased on the 21st day with the cells incubated with LPS and HKY (2.1fold higher than the control group) |
| 3.4.22.B19 | more |
pathogenic Yersinia spp. secrete the effector YopJ into host cells to counteract cytokine production and to induce programmed cell death. YopJ achieves these aims by inactivating mitogen-activated protein kinase and nuclear factor kappaB signaling pathways. YopJ binds to members of the MAPK kinase family and is predicted to have protease activity toward ubiquitin-like proteins |
| 3.4.22.B19 | Rac1 + H2O |
- |
| 3.4.22.B19 | RhoA + H2O |
- |
| 3.4.22.B19 | TRAF2-ubiquitin + H2O |
- |
