EC Number   |
Metals/Ions |
Reference |
|---|
   3.4.22.52 | Ca2+ |
mu-calpain is freely diffusible in the cytoplasm at resting Ca2+ concentrations but binds within seconds at high Ca2+ concentrations. Ca2+ concentration has to be raised to above 0.002 mM for more than 1 min to initiate detectable autolysis of mu-calpain and to activate appreciable proteolytic activity. If Ca2+ concentration is raised sufficiently for long enough to initiate substantial autolysis of mu-calpain, the Ca2+ sensitivity of the proteolytic activity is greatly increased and it remains active even at 300 nM Ca2+, with activity only ceasing if the Ca2+ concentration is decreased to about 50 nM Ca2+ |
670009 |
| 3.4.22.52 | Ca2+ |
needed for enzyme activation |
731990 |
| 3.4.22.52 | Ca2+ |
required |
708051 |
| 3.4.22.52 | Ca2+ |
required for activity |
708257 |
| 3.4.22.52 | Ca2+ |
requires 0.053 mM Ca2+ for half-maximal activity. Activation by Ca2+ promotes the separation of the two subunits of the expressed recombinant protein |
643976 |
| 3.4.22.52 | Ca2+ |
requires a micromolar concentration of Ca2+ for activity |
707065 |
| 3.4.22.52 | Ca2+ |
requires a micromolar concentration of calcium ions |
707531 |
| 3.4.22.52 | Ca2+ |
sensitive to the frequency of fast Ca2+ oscillations in vitro |
643997 |
| 3.4.22.52 | Mn2+ |
slight activation |
643988 |
| 3.4.22.52 | Mn2+ |
synergistic acivation in combination with Ca2+ |
643967 |
