EC Number   |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate  |
Reference |
|---|
   1.13.12.5 | 0.0016 |
- |
coelenterazine |
mutant enzyme A55T/C124A/S130A/K136R/A143M/M185V/M253L/S287L |
676939 |
| 1.13.12.5 | 0.00214 |
- |
coelenterazine |
K0.5 value, Hill coefficient 1.8, pH 7.5, temperature not specified in the publication |
765264 |
| 1.13.12.5 | 0.0025 |
- |
coelenterazine |
wild-type enzyme, pH 7.2, 25°C |
744017 |
| 1.13.12.5 | 0.0029 |
- |
coelenterazine |
wild-type enzyme |
676939 |
| 1.13.12.5 | 0.00293 |
- |
coelenterazine |
pH 7.2, 37°C, recombinant engineered mutant super RLuc 8 |
744813 |
| 1.13.12.5 | 0.003 |
- |
coelenterazine |
modified enzyme FcUni-RLuc, pH 7.2, 25°C |
744017 |
| 1.13.12.5 | 0.0031 |
- |
coelenterazine |
pH 7.8, 25°C, recombinant His-tagged wild-type enzyme |
744253 |
| 1.13.12.5 | 0.0068 |
0.0103 |
coelenterazine |
pH 7.8, 25°C, recombinant His-tagged wild-type enzyme in presence of 13.3-26.7 mM 1-butyl-3-methylimidazolium tetrafluoroborate |
744253 |
| 1.13.12.5 | 0.00728 |
- |
coelenterazine |
pH 7.2, 37°C, recombinant modified enzyme C-SRLuc8 |
744428 |
| 1.13.12.5 | 0.0097 |
0.0119 |
coelenterazine |
pH 7.8, 25°C, recombinant His-tagged wild-type enzyme in presence of 4.8-9.7 mM 1-butyl-3-methylimidazoliumhexafluorophosphate |
744253 |
