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Information on EC - Renilla-type luciferase

for references in articles please use BRENDA:EC1.13.12.5
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IUBMB Comments
From the soft coral coelenterate Renilla reniformis. The luciferin is bound to a luciferin-binding protein (BP-LH2). The bioluminescent reaction between the luciferin complex, luciferase and oxygen is triggered by calcium ions. In vivo, the excited state luciferin---luciferase complex undergoes the process of nonradiative energy transfer to an accessory protein, Renilla green fluorescent protein, which results in green bioluminescence. In vitro, Renilla luciferase emits blue light in the absence of any green fluorescent protein.
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The enzyme appears in viruses and cellular organisms
Reaction Schemes
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excited coelenteramide h monoanion
aequorin, renilla luciferase, gaussia luciferase, gaussia princeps luciferase, renilla reniformis luciferase, clytin, rluc8, oplophorus luciferase, bfp-aq, renilla-luciferase, more
coelenterazine h + O2 = coelenterazine h dioxetanone
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coelenterazine h + O2 = excited coelenteramide h monoanion + CO2
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coelenterazine h dioxetanone = excited coelenteramide h monoanion + CO2
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