EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
---|
2.3.1.54 | -999 |
- |
more |
kinetics and equilibrium constant for the enzyme's interaction with activating enzyme PFL-AE, the interaction is very slow and rate-limited by large conformational changes, circular dichroism study |
745316 |
2.3.1.54 | 0.964 |
- |
pyruvate |
pH 7.6, 30°C, recombinant mutant A273C |
672694 |
2.3.1.54 | 3.6 |
- |
pyruvate |
pH 7.6, 30°C, recombinant mutant E336C |
672694 |
2.3.1.54 | 3.55 |
- |
pyruvate |
pH 7.6, 30°C, recombinant mutant E400I |
672694 |
2.3.1.54 | 2.37 |
- |
pyruvate |
pH 7.6, 30°C, recombinant wild-type enzyme |
672694 |
2.3.1.54 | 0.012 |
- |
CoA |
pyruvate |
487218 |
2.3.1.54 | 0.6 |
- |
pyruvate |
pyruvate-formate exchange |
487218 |
2.3.1.54 | 20 |
- |
formate |
pyruvate-formate exchange |
487218 |
2.3.1.54 | -999 |
- |
more |
steady-state kinetics of wild-type and mutant enzymes |
672694 |