2.3.1.54	0.0068	-	CoA	-	487220, 487221	
2.3.1.54	0.051	-	acetyl-CoA	-	487220, 487221	
2.3.1.54	0.26	-	acetyl-CoA	-	487218	
2.3.1.54	1.6	-	pyruvate	-	487218, 487234	
2.3.1.54	2	-	pyruvate	-	487221, 487234	
2.3.1.54	2.05	-	pyruvate	-	487220	
2.3.1.54	2.6	-	pyruvate	-	487234	
2.3.1.54	10	-	formate	-	487218	
2.3.1.54	24	-	formate	-	487220, 487221	
2.3.1.54	24.5	-	formate	-	487220	
2.3.1.54	additional information	-	additional information	kinetics and equilibrium constant for the enzyme's interaction with activating enzyme PFL-AE, the interaction is very slow and rate-limited by large conformational changes, circular dichroism study	745316	
2.3.1.54	0.964	-	pyruvate	pH 7.6, 30°C, recombinant mutant A273C	672694	
2.3.1.54	3.6	-	pyruvate	pH 7.6, 30°C, recombinant mutant E336C	672694	
2.3.1.54	3.55	-	pyruvate	pH 7.6, 30°C, recombinant mutant E400I	672694	
2.3.1.54	2.37	-	pyruvate	pH 7.6, 30°C, recombinant wild-type enzyme	672694	
2.3.1.54	0.012	-	CoA	pyruvate	487218	
2.3.1.54	0.6	-	pyruvate	pyruvate-formate exchange	487218	
2.3.1.54	20	-	formate	pyruvate-formate exchange	487218	
2.3.1.54	additional information	-	additional information	steady-state kinetics of wild-type and mutant enzymes	672694