EC Number   |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Reference |
|---|
    1.13.11.27 | 0.52 |
- |
phenylpyruvate |
- |
395384 |
| 1.13.11.27 | 0.551 |
- |
4-hydroxyphenylpyruvate |
recombinant mutant Q286E, pH and temperature not specified in the publication |
725432 |
| 1.13.11.27 | 0.6 |
- |
4-hydroxyphenylpyruvate |
mutant Q334A/N363Q, pH 6.5, 37°C |
764109 |
| 1.13.11.27 | 0.9 |
- |
4-hydroxyphenylpyruvate |
mutant Q265E, pH 6.5, 37°C |
764109 |
| 1.13.11.27 | 11 |
- |
4-hydroxyphenylpyruvate |
Solvent is H2O. Steady-state analyses of 4-hydroxyphenylpyruvate dioxygenase using proteo-HPP, 2,3,5,6-tetradeutero-HPP, and proteo-HPP in deuterium oxide are carried out in 1 ml assays on a Hasetech oxygen electrode. The assay mixture include ferrous sulfate (10 microM), DTT (1mM), HPPD (500 nM), and HPP (0-1 mM) in 20 mM HEPES, pH (pD) 7.0, under conditions of atmospheric oxygen (350 microM at 5°C) |
672033 |
| 1.13.11.27 | 22 |
- |
4-hydroxyphenylpyruvate |
Solvent is H2O. Steady-state analyses of 4-hydroxyphenylpyruvate dioxygenase using proteo-HPP, 2,3,5,6-tetradeutero-HPP, and proteo-HPP in deuterium oxide are carried out in 1 ml assays on a Hasetech oxygen electrode. The assay mixture include ferrous sulfate (10 microM), DTT (1mM), HPPD (500 nM), and HPP (0-1 mM) in 20 mM HEPES, pH (pD) 7.0, under conditions of atmospheric oxygen (350 microM at 5°C) |
672033 |
| 1.13.11.27 | 54 |
- |
4-hydroxyphenylpyruvate |
Solvent is D2O. Steady-state analyses of 4-hydroxyphenylpyruvate dioxygenase using proteo-HPP, 2,3,5,6-tetradeutero-HPP, and proteo-HPP in deuterium oxide are carried out in 1 ml assays on a Hasetech oxygen electrode. The assay mixture include ferrous sulfate (10 microM), DTT (1mM), HPPD (500 nM), and HPP (0-1 mM) in 20 mM HEPES, pH (pD) 7.0, under conditions of atmospheric oxygen (350 microM at 5°C) |
672033 |
