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Literature summary for 1.13.11.27 extracted from

  • Huang, C.W.; Hwang, C.C.; Chang, Y.L.; Liu, J.T.; Wu, S.P.; Huang, K.L.; Huang, W.M.; Lee, H.J.
    Functional role of residues involved in substrate binding of human 4-hydroxyphenylpyruvate dioxygenase (2021), Biochem. J., 478, 2201-2215 .
    View publication on PubMed
Search for more literature for 1.13.11.27

Crystallization (Commentary)

Crystallization (Comment) Organism
comparison of Arabidopsis thaiana and human enzyme. In human, the carboxyl group of substrate 4-hydroxyphenylpyruvate interacts by a H-bond network formed by Gln334, metal-binding ligand Glu349, and Asn363 in the C-terminal helix. The 4-hydroxyl group of the substrate interacts with Gln251 and Gln265 Homo sapiens

Protein Variants

Protein Variants Comment Organism
N363A about 20% of wild-type activity Homo sapiens
N363D about 6% of wild-type activity Homo sapiens
N363Q about 7% of wild-type activity Homo sapiens
Q251E almost complete loss of activity Homo sapiens
Q265E about 5% of wild-type activity Homo sapiens
Q334A relative activity and substrate-binding affinity are preserved Homo sapiens
Q334A/N363D about 5% of wild-type activity Homo sapiens
Q334A/N363Q about 3% of wild-type activity Homo sapiens
Q334N about 3% of wild-type activity Homo sapiens
Q334N/N363Q about 2% of wild-type activity Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
 4-hydroxyphenylpyruvate wild-type, pH 6.5, 37°C Homo sapiens
0.2
-
 4-hydroxyphenylpyruvate mutant Q334N, pH 6.5, 37°C Homo sapiens
0.3
-
 4-hydroxyphenylpyruvate mutant N363A, pH 6.5, 37°C Homo sapiens
0.3
-
 4-hydroxyphenylpyruvate mutant Q334A, pH 6.5, 37°C Homo sapiens
0.4
-
 4-hydroxyphenylpyruvate mutant N363Q, pH 6.5, 37°C Homo sapiens
0.5
-
 4-hydroxyphenylpyruvate mutant N363D, pH 6.5, 37°C Homo sapiens
0.5
-
 4-hydroxyphenylpyruvate mutant Q334A/N363D, pH 6.5, 37°C Homo sapiens
0.5
-
 4-hydroxyphenylpyruvate mutant Q334N/N363Q, pH 6.5, 37°C Homo sapiens
0.6
-
 4-hydroxyphenylpyruvate mutant Q334A/N363Q, pH 6.5, 37°C Homo sapiens
0.9
-
 4-hydroxyphenylpyruvate mutant Q265E, pH 6.5, 37°C Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens P32754
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-hydroxyphenylpyruvate + O2
-
 Homo sapiens homogentisate + CO2
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
52.6
-
 melting temperature, wild-type Homo sapiens
54.3
-
 melting temperature, wild-type in presence of Fe2+ Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.06
-
 4-hydroxyphenylpyruvate mutant Q334N, pH 6.5, 37°C Homo sapiens
0.09
-
 4-hydroxyphenylpyruvate mutant Q334N/N363Q, pH 6.5, 37°C Homo sapiens
0.1
-
 4-hydroxyphenylpyruvate mutant Q265E, pH 6.5, 37°C Homo sapiens
0.2
-
 4-hydroxyphenylpyruvate mutant Q334A/N363D, pH 6.5, 37°C Homo sapiens
0.2
-
 4-hydroxyphenylpyruvate mutant Q334A/N363Q, pH 6.5, 37°C Homo sapiens
0.3
-
 4-hydroxyphenylpyruvate mutant N363D, pH 6.5, 37°C Homo sapiens
0.3
-
 4-hydroxyphenylpyruvate mutant N363Q, pH 6.5, 37°C Homo sapiens
0.6
-
 4-hydroxyphenylpyruvate mutant N363A, pH 6.5, 37°C Homo sapiens
1
-
 4-hydroxyphenylpyruvate mutant Q334A, pH 6.5, 37°C Homo sapiens
2
-
 4-hydroxyphenylpyruvate wild-type, pH 6.5, 37°C Homo sapiens

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.2
-
 4-hydroxyphenylpyruvate mutant Q265E, pH 6.5, 37°C Homo sapiens
0.2
-
 4-hydroxyphenylpyruvate mutant Q334N/N363Q, pH 6.5, 37°C Homo sapiens
0.3
-
 4-hydroxyphenylpyruvate mutant Q334A/N363Q, pH 6.5, 37°C Homo sapiens
0.3
-
 4-hydroxyphenylpyruvate mutant Q334N, pH 6.5, 37°C Homo sapiens
0.5
-
 4-hydroxyphenylpyruvate mutant Q334A/N363D, pH 6.5, 37°C Homo sapiens
0.6
-
 4-hydroxyphenylpyruvate mutant N363D, pH 6.5, 37°C Homo sapiens
0.7
-
 4-hydroxyphenylpyruvate mutant N363Q, pH 6.5, 37°C Homo sapiens
2
-
 4-hydroxyphenylpyruvate mutant N363A, pH 6.5, 37°C Homo sapiens
4
-
 4-hydroxyphenylpyruvate mutant Q334A, pH 6.5, 37°C Homo sapiens
10
-
 4-hydroxyphenylpyruvate wild-type, pH 6.5, 37°C Homo sapiens