EC Number   |
General Stability |
Reference |
|---|
    1.1.1.37 | addition of NAD+, NADH or oxaloacetate stabilizes the enzyme |
286657 |
| 1.1.1.37 | at 10 mM MgCl2, residual activity after 24 h in H2O is less than 20% at 4°C, whereas in D2O the protein is stable with 100% residual activity |
727410 |
| 1.1.1.37 | at 4°C, the protein at pH 7.0 is stable between 0.5 M and 0.9 M MgCl2. At pH 8.0, it is stable between 0.05 M and 1.2 M MgCl2 |
727410 |
| 1.1.1.37 | both Mg2+ and NADH stabilizes the 127000 Da MW form |
286628 |
| 1.1.1.37 | Ca2+, Mg2+, Li+, NH4+, Na+, K+, Rb+ and Cs+ stabilize the enzyme in order of decreasing efficiency |
726935 |
| 1.1.1.37 | completely stable in absence of salt |
654906 |
| 1.1.1.37 | fast dynamics of the enzyme is measured by neutron scattering under various solvent conditions influencing protein stability |
728706 |
| 1.1.1.37 | in KCl at 25°C, 50% residual activity is observed after 24 h for mutant enzyme E243R in 2.3 M salt, compared to 1.3 M salt for wild-type enzyme. In KCl at 4°C, the mutant shows 50% stability in 1.1 M salt |
727406 |
| 1.1.1.37 | mitochondrial malate dehydrogenase is very unstable |
286655 |
| 1.1.1.37 | only stable in highly concentrated solutions of certain salts |
286672 |
