EC Number   |
Activating Compound |
Reference  |
|---|
   1.1.1.244 | Activator protein |
activation in presence of Mg2+, in vitro: 3 mol soluble activator protein per mol enzyme, in vivo estimated as 1 mol per 17.5 mol enzyme, MW 50000 with two subunits of MW 27000 each, in presence of activator protein and Mg2+ enzyme possesses a high affinity active site for alcohol and NAD+, up to 40fold increase of activity, primarily of Vmax, slight decrease of Km-value for methanol, enzyme/activator-interaction is dilution-sensitive, no stimulation of formaldehyde reduction |
286375 |
| 1.1.1.244 | Activator protein |
- |
286376 |
| 1.1.1.244 | Activator protein |
each activator protein subunit binds one molecule of NADH, activator may facilitate oxidation of reduced enzyme bound NADH cofactor |
286377 |
| 1.1.1.244 | more |
strongly stimulated by the ACT protein |
656074, 656101 |
| 1.1.1.244 | activator protein ACT |
all three isozymes are activated by ACT, which is encoded by gene act, overview |
726329 |
| 1.1.1.244 | NudF protein |
can be catalytically stimulated by the Bacillus subtilis NudF protein in vitro |
726329 |
| 1.1.1.244 | more |
Mdh2 from Cupriavidus necator does not require activation by and is insensitive to known activators such as Bacillus methanolicus ACT and Escherichia coli Nudix hydrolase NudF, or putative native Cupriavidus necator activators in the Nudix family under mesophilic condition. The enzyme exhibits higher or comparable activity and affinity toward methanol relative to the Bacillus methanolicus Mdh with or without activator ACT in a wide range of temperatures |
741715 |
| 1.1.1.244 | Act |
activator protein, UniProt ID I3EA59, the in vitro activity of Bacillus methanolicus Mdh is increased by the endogenous activator protein Act, a Nudix hydrolase, in a pH-dependent manner. Act increases the Vmax of the enzyme and decreases the Km for methanol, the increase was 23fold at pH 7.4 and 50fold at pH 9.0 |
742514 |
| 1.1.1.244 | activator protein ACT |
UniProt ID I3EA59, the in vitro activity of Bacillus methanolicus Mdh is increased by the endogenous activator protein Act, a Nudix hydrolase, in a pH-dependent manner. Act increases the Vmax of the enzyme and decreases the Km for methanol, the increase is 20fold at pH 7.4 and 9fold at pH 9.0 |
742514 |
| 1.1.1.244 | more |
the enzyme is activated by several Nudix hydrolases, relative activities of Adh enzymes, overview |
742514 |
