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Information on EC 1.1.1.244 - methanol dehydrogenase
for references in articles please use BRENDA:EC1.1.1.244
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EC Tree 1 Oxidoreductases
1.1 Acting on the CH-OH group of donors
1.1.1 With NAD+ or NADP+ as acceptor
1.1.1.244 methanol dehydrogenase
The expected taxonomic range for this enzyme is: Bacteria, Archaea
- 1.1.1.244
- methylobacterium
- formaldehyde
- quinone
- methane
- methanotrophs
- pqq
- extorquens
-
quinoproteins
- denitrificans
-
methylotrophy
- methylamine
-
paracoccus
-
lanthanide
- methylophilus
- hyphomicrobium
- methanolicus
- methylomonas
- methylosinus
- methylococcus
-
methane-oxidizing
-
methanol-grown
- trichosporium
-
lanthanide-dependent
-
pqq-dependent
- methylomicrobium
-
pink-pigmented
-
pyrrolo-quinoline
-
rump
- methylocystis
- methylophaga
- methylobacter
-
xoxf-type
-
pqq-containing
- organophilum
-
phyllosphere
-
quinone-dependent
- methylacidiphilum
-
rare-earth
- ch3oh
-
dye-linked
- biotechnology
showSynonyms
methanol dehydrogenase, nad-dependent mdh, nad-dependent methanol dehydrogenase, lxmdh, methanol dehydrogenase 2, group iii nad-dependent alcohol dehydrogenase, more
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SYNONYM 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
activator-independent methanol dehydrogenase
ADH
BFZC1_05383
dehydrogenase, methanol
-
-
-
-
group III NAD-dependent alcohol dehydrogenase
lxmdh
MDH
Mdh1
MDH2
Mdh3
MEDH
-
-
-
-
methanol dehydrogenase 2
NAD+ dependent methanol dehydrogenase
NAD-dependent MDH
NAD-dependent methanol dehydrogenase
additional information
additional information
-
not identical with methanol dehydrogenase which uses pyrroloquinoline quinone as prosthetic group, EC 1.1.99.8
additional information
-
not identical with methanol dehydrogenase which uses pyrroloquinoline quinone as prosthetic group, EC 1.1.99.8
additional information
-
not identical with methanol dehydrogenase which uses pyrroloquinoline quinone as prosthetic group, EC 1.1.99.8
-
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REACTION
REACTION DIAGRAM
COMMENTARY
ORGANISM
UNIPROT
LITERATURE
methanol + NAD+ = formaldehyde + NADH + H+
ping-pong mechanism in the absence of ACT protein
-
methanol + NAD+ = formaldehyde + NADH + H+
ping-pong mechanism in the absence of ACT protein
-
-
methanol + NAD+ = formaldehyde + NADH + H+
-
-
-
-
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REACTION TYPE
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
oxidation
-
-
-
-
redox reaction
-
-
-
-
reduction
-
-
-
-
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PATHWAY SOURCE
PATHWAYS
MetaCyc
methanol oxidation to carbon dioxide, methanol oxidation to formaldehyde II
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SYSTEMATIC NAME
IUBMB Comments
methanol:NAD+ oxidoreductase
-
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CAS REGISTRY NUMBER
COMMENTARY
74506-37-9
-
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SUBSTRATE
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
isopropanol + NAD+
isopropanal + NADH + H+
-
Substrates: low activity
Products: -
Products: -
?
1,3-propandiol + NAD+
? + NADH + H+
-
Substrates: very low activity
Products: -
Products: -
?
1,3-propandiol + NAD+
? + NADH + H+
-
Substrates: low activity
Products: -
Products: -
?
butanol + NAD+
butyraldehyde + NADH + H+
-
Substrates: -
Products: -
Products: -
?
butanol + NAD+
butyraldehyde + NADH + H+
Lysinibacillus xylanilyticus KCTC 13423
-
Substrates: -
Products: -
Products: -
?
ethanol + NAD+
acetaldehyde + NADH + H+
Substrates: -
Products: -
Products: -
r
ethanol + NAD+
acetaldehyde + NADH + H+
Substrates: -
Products: -
Products: -
r
ethanol + NAD+
acetaldehyde + NADH + H+
-
Substrates: -
Products: -
Products: -
?
ethanol + NAD+
acetaldehyde + NADH + H+
Lysinibacillus xylanilyticus KCTC 13423
-
Substrates: -
Products: -
Products: -
?
ethanol + NAD+
ethanal + NADH
-
Substrates: best substrate
Products: -
Products: -
?
ethanol + NAD+
ethanal + NADH
-
Substrates: best substrate
Products: -
Products: -
r
formaldehyde + NADH + H+
methanol + NAD+
Substrates: -
Products: -
Products: -
r
formaldehyde + NADH + H+
methanol + NAD+
-
Substrates: Mdhs generally show higher activity and affinity for formaldehyde compared to methanol
Products: -
Products: -
r
formaldehyde + NADH + H+
methanol + NAD+
Substrates: key step for both toxic formaldehyde elimination
Products: -
Products: -
r
formaldehyde + NADH + H+
methanol + NAD+
Substrates: -
Products: -
Products: -
r
formaldehyde + NADH + H+
methanol + NAD+
Substrates: key step for both toxic formaldehyde elimination
Products: -
Products: -
r
formaldehyde + NADH + H+
methanol + NAD+
-
Substrates: Mdhs generally show higher activity and affinity for formaldehyde compared to methanol
Products: -
Products: -
r
formaldehyde + NADH + H+
methanol + NAD+
-
Substrates: Mdhs generally show higher activity and affinity for formaldehyde compared to methanol
Products: -
Products: -
r
methanol + NAD+
formaldehyde + NADH + H+
-
Substrates: -
Products: -
Products: -
?
methanol + NAD+
formaldehyde + NADH + H+
Substrates: -
Products: -
Products: -
r
methanol + NAD+
formaldehyde + NADH + H+
Substrates: -
Products: -
Products: -
r
methanol + NAD+
formaldehyde + NADH + H+
Substrates: -
Products: -
Products: -
r
methanol + NAD+
formaldehyde + NADH + H+
Substrates: -
Products: -
Products: -
r
methanol + NAD+
formaldehyde + NADH + H+
-
Substrates: 33% of activity with ethanol
Products: -
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r
methanol + NAD+
formaldehyde + NADH + H+
-
Substrates: 44% of activity with ethanol, NADP+ cannot replace NAD+
Products: -
Products: -
?
methanol + NAD+
formaldehyde + NADH + H+
-
Substrates: no activity with 2-propanol, 2,3-butandiol, mannitol and glycerol
Products: -
Products: -
?
methanol + NAD+
formaldehyde + NADH + H+
-
Substrates: involved in initial methanol oxidation
Products: -
Products: -
r
methanol + NAD+
formaldehyde + NADH + H+
-
Substrates: very low activity, Mdhs generally show higher activity and affinity for formaldehyde compared to methanol
Products: -
Products: -
r
methanol + NAD+
formaldehyde + NADH + H+
-
Substrates: very low activity. Mdhs generally show higher activity and affinity for formaldehyde compared to methanol
Products: -
Products: -
r
methanol + NAD+
formaldehyde + NADH + H+
Substrates: in nature, methanol dehydrogenase (Mdh), which converts methanol to formaldehyde, highly favors the reverse reaction
Products: -
Products: -
r
methanol + NAD+
formaldehyde + NADH + H+
Substrates: key step for ethanol production in bacterial methylotrophy
Products: -
Products: -
r
methanol + NAD+
formaldehyde + NADH + H+
Substrates: -
Products: -
Products: -
r
methanol + NAD+
formaldehyde + NADH + H+
-
Substrates: -
Products: -
Products: -
?
methanol + NAD+
formaldehyde + NADH + H+
Substrates: -
Products: -
Products: -
r
methanol + NAD+
formaldehyde + NADH + H+
Substrates: key step for ethanol production in bacterial methylotrophy
Products: -
Products: -
r
methanol + NAD+
formaldehyde + NADH + H+
-
Substrates: -
Products: -
Products: -
r
methanol + NAD+
formaldehyde + NADH + H+
-
Substrates: very low activity, Mdhs generally show higher activity and affinity for formaldehyde compared to methanol
Products: -
Products: -
r
methanol + NAD+
formaldehyde + NADH + H+
-
Substrates: very low activity. Mdhs generally show higher activity and affinity for formaldehyde compared to methanol
Products: -
Products: -
r
methanol + NAD+
formaldehyde + NADH + H+
Substrates: -
Products: -
Products: -
r
methanol + NAD+
formaldehyde + NADH + H+
Substrates: -
Products: -
Products: -
r
methanol + NAD+
formaldehyde + NADH + H+
Substrates: in nature, methanol dehydrogenase (Mdh), which converts methanol to formaldehyde, highly favors the reverse reaction
Products: -
Products: -
r
methanol + NAD+
formaldehyde + NADH + H+
-
Substrates: -
Products: -
Products: -
r
methanol + NAD+
formaldehyde + NADH + H+
-
Substrates: very low activity, Mdhs generally show higher activity and affinity for formaldehyde compared to methanol
Products: -
Products: -
r
methanol + NAD+
formaldehyde + NADH + H+
-
Substrates: very low activity. Mdhs generally show higher activity and affinity for formaldehyde compared to methanol
Products: -
Products: -
r
methanol + NAD+
formaldehyde + NADH + H+
Substrates: -
Products: -
Products: -
r
methanol + NAD+
formaldehyde + NADH + H+
Substrates: XoxF may not be involved in methylotrophy
Products: -
Products: -
?
methanol + NAD+
formaldehyde + NADH + H+
Substrates: -
Products: -
Products: -
r
methanol + NAD+
formaldehyde + NADH + H+
Substrates: -
Products: -
Products: -
r
methanol + NAD+
formaldehyde + NADH + H+
-
Substrates: -
Products: -
Products: -
r
methanol + NAD+
formaldehyde + NADH + H+
-
Substrates: -
Products: -
Products: -
r
methanol + NAD+
formaldehyde + NADH + H+
-
Substrates: -
Products: -
Products: -
r
methanol + NAD+
formaldehyde + NADH + H+
-
Substrates: -
Products: -
Products: -
r
methanol + NAD+
formaldehyde + NADH + H+
-
Substrates: -
Products: -
Products: -
r
methanol + NAD+
formaldehyde + NADH + H+
-
Substrates: -
Products: -
Products: -
r
methanol + NAD+
formaldehyde + NADH + H+
-
Substrates: -
Products: -
Products: -
r
methanol + NAD+
formaldehyde + NADH + H+
Substrates: -
Products: -
Products: -
r
methanol + NAD+
formaldehyde + NADH + H+
-
Substrates: -
Products: -
Products: -
?
methanol + NAD+
formaldehyde + NADH + H+
Lysinibacillus xylanilyticus KCTC 13423
-
Substrates: -
Products: -
Products: -
?
methanol + NAD+
formaldehyde + NADH + H+
-
Substrates: -
Products: -
Products: -
r
methanol + NADP+
formaldehyde + NADPH + H+
I3DTM5, I3E2P9
Substrates: Mdh enzyme mutant D41G, no activity of the wild-type enzyme with NADP(H)
Products: -
Products: -
r
methanol + NADP+
formaldehyde + NADPH + H+
I3DTM5, I3E2P9
Substrates: Mdh enzyme mutant D98G, no activity of the wild-type enzyme with NADP(H)
Products: -
Products: -
r
methanol + NADP+
formaldehyde + NADPH + H+
I3DTM5, I3E2P9
Substrates: Mdh enzyme mutant D98G, no activity of the wild-type enzyme with NADP(H)
Products: -
Products: -
r
methanol + NADP+
formaldehyde + NADPH + H+
I3DTM5, I3E2P9
Substrates: Mdh enzyme mutant D41G, no activity of the wild-type enzyme with NADP(H)
Products: -
Products: -
r
n-butanol + NAD+
butyraldehyde + NADH + H+
Substrates: -
Products: -
Products: -
r
n-butanol + NAD+
butyraldehyde + NADH + H+
Substrates: -
Products: -
Products: -
r
n-butanol + NAD+
n-butanal + NADH
-
Substrates: 87% of activity with ethanol
Products: -
Products: -
?
n-butanol + NAD+
n-butanal + NADH
-
Substrates: 87% of activity with ethanol
Products: -
Products: -
r
n-propanol + NAD+
n-propanal + NADH
-
Substrates: 71% of activity with ethanol
Products: -
Products: -
?
n-propanol + NAD+
n-propanal + NADH
-
Substrates: 71% of activity with ethanol
Products: -
Products: -
r
n-propanol + NAD+
propionaldehyde + NADH + H+
-
Substrates: -
Products: -
Products: -
?
n-propanol + NAD+
propionaldehyde + NADH + H+
Lysinibacillus xylanilyticus KCTC 13423
-
Substrates: -
Products: -
Products: -
?
propanol + NAD+
propionaldehyde + NADH + H+
Substrates: -
Products: -
Products: -
r
propanol + NAD+
propionaldehyde + NADH + H+
Substrates: -
Products: -
Products: -
r
additional information
?
-
-
Substrates: all Mdh isozymes catalyze the oxidation of methanol, but the catalytic activity for methanol is considerably lower than for most other alcohols tested. The isozymes exhibit a broad and different substrate specificity range, and display both dehydrogenase and reductase activities. Isozyme Mdh3 shows the highest activity of all isozymes
Products: -
Products: -
?
additional information
?
-
Substrates: efficient coupling with the irreversible sequestration of formaldehyde by 3-hexulose-6-phosphate synthase (Hps) and 6-phospho-3-hexuloseisomerase (Phi) serves as the key driving force to pull the pathway equilibrium toward central metabolism
Products: -
Products: -
?
additional information
?
-
I3DTM5
Substrates: poor activity with 2-propanol and 1,2-popanediol
Products: -
Products: -
?
additional information
?
-
Substrates: poor activity with 2-propanol and 1,2-popanediol
Products: -
Products: -
?
additional information
?
-
-
Substrates: poor activity with 2-propanol and 1,2-popanediol
Products: -
Products: -
?
additional information
?
-
-
Substrates: all Mdh isozymes catalyze the oxidation of methanol, but the catalytic activity for methanol is considerably lower than for most other alcohols tested. The isozymes exhibit a broad and different substrate specificity range, and display both dehydrogenase and reductase activities. Isozyme Mdh3 shows the highest activity of all isozymes
Products: -
Products: -
?
additional information
?
-
I3DTM5
Substrates: poor activity with 2-propanol and 1,2-popanediol
Products: -
Products: -
?
additional information
?
-
Substrates: poor activity with 2-propanol and 1,2-popanediol
Products: -
Products: -
?
additional information
?
-
Substrates: efficient coupling with the irreversible sequestration of formaldehyde by 3-hexulose-6-phosphate synthase (Hps) and 6-phospho-3-hexuloseisomerase (Phi) serves as the key driving force to pull the pathway equilibrium toward central metabolism
Products: -
Products: -
?
additional information
?
-
-
Substrates: all Mdh isozymes catalyze the oxidation of methanol, but the catalytic activity for methanol is considerably lower than for most other alcohols tested. The isozymes exhibit a broad and different substrate specificity range, and display both dehydrogenase and reductase activities. Isozyme Mdh3 shows the highest activity of all isozymes
Products: -
Products: -
?
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NATURAL SUBSTRATE
NATURAL PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
LITERATURE
COMMENTARY
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
formaldehyde + NADH + H+
methanol + NAD+
Substrates: key step for both toxic formaldehyde elimination
Products: -
Products: -
r
formaldehyde + NADH + H+
methanol + NAD+
Substrates: key step for both toxic formaldehyde elimination
Products: -
Products: -
r
methanol + NAD+
formaldehyde + NADH + H+
-
Substrates: -
Products: -
Products: -
?
methanol + NAD+
formaldehyde + NADH + H+
Substrates: -
Products: -
Products: -
r
methanol + NAD+
formaldehyde + NADH + H+
-
Substrates: involved in initial methanol oxidation
Products: -
Products: -
r
methanol + NAD+
formaldehyde + NADH + H+
Substrates: in nature, methanol dehydrogenase (Mdh), which converts methanol to formaldehyde, highly favors the reverse reaction
Products: -
Products: -
r
methanol + NAD+
formaldehyde + NADH + H+
Substrates: key step for ethanol production in bacterial methylotrophy
Products: -
Products: -
r
methanol + NAD+
formaldehyde + NADH + H+
-
Substrates: -
Products: -
Products: -
?
methanol + NAD+
formaldehyde + NADH + H+
Substrates: key step for ethanol production in bacterial methylotrophy
Products: -
Products: -
r
methanol + NAD+
formaldehyde + NADH + H+
-
Substrates: -
Products: -
Products: -
r
methanol + NAD+
formaldehyde + NADH + H+
Substrates: -
Products: -
Products: -
r
methanol + NAD+
formaldehyde + NADH + H+
Substrates: in nature, methanol dehydrogenase (Mdh), which converts methanol to formaldehyde, highly favors the reverse reaction
Products: -
Products: -
r
methanol + NAD+
formaldehyde + NADH + H+
-
Substrates: -
Products: -
Products: -
r
methanol + NAD+
formaldehyde + NADH + H+
Substrates: -
Products: -
Products: -
r
methanol + NAD+
formaldehyde + NADH + H+
Substrates: -
Products: -
Products: -
r
methanol + NAD+
formaldehyde + NADH + H+
Substrates: -
Products: -
Products: -
r
methanol + NAD+
formaldehyde + NADH + H+
-
Substrates: -
Products: -
Products: -
r
methanol + NAD+
formaldehyde + NADH + H+
-
Substrates: -
Products: -
Products: -
r
methanol + NAD+
formaldehyde + NADH + H+
-
Substrates: -
Products: -
Products: -
r
methanol + NAD+
formaldehyde + NADH + H+
-
Substrates: -
Products: -
Products: -
r
methanol + NAD+
formaldehyde + NADH + H+
-
Substrates: -
Products: -
Products: -
r
methanol + NAD+
formaldehyde + NADH + H+
-
Substrates: -
Products: -
Products: -
r
methanol + NAD+
formaldehyde + NADH + H+
-
Substrates: -
Products: -
Products: -
r
methanol + NAD+
formaldehyde + NADH + H+
Substrates: -
Products: -
Products: -
r
methanol + NAD+
formaldehyde + NADH + H+
-
Substrates: -
Products: -
Products: -
?
methanol + NAD+
formaldehyde + NADH + H+
Lysinibacillus xylanilyticus KCTC 13423
-
Substrates: -
Products: -
Products: -
?
methanol + NAD+
formaldehyde + NADH + H+
-
Substrates: -
Products: -
Products: -
r
additional information
?
-
Substrates: efficient coupling with the irreversible sequestration of formaldehyde by 3-hexulose-6-phosphate synthase (Hps) and 6-phospho-3-hexuloseisomerase (Phi) serves as the key driving force to pull the pathway equilibrium toward central metabolism
Products: -
Products: -
?
additional information
?
-
Substrates: efficient coupling with the irreversible sequestration of formaldehyde by 3-hexulose-6-phosphate synthase (Hps) and 6-phospho-3-hexuloseisomerase (Phi) serves as the key driving force to pull the pathway equilibrium toward central metabolism
Products: -
Products: -
?
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COFACTOR
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
IMAGE
NAD+
-
tightly but not covalentely bound to enzyme, a second NAD+ is required for the regeneration of bound NADH
additional information
-
enzyme contains most probably 1 NAD(H) cofactor molecule/subunit serving as primary electron acceptor, exogenous NAD+ (coenzyme) is responsible for reoxidation of enzyme bound NADH
-
additional information
no methanol oxidation activity is detected when NADP+ is used
-
additional information
-
no methanol oxidation activity is detected when NADP+ is used
-
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METALS and IONS
ORGANISM
UNIPROT
COMMENTARY
LITERATURE
Mg2+
Zn2+
additional information
Mg2+
-
required, 1-2 mol per mol subunit, atomic absorption spectroscopy, activation by activator protein depends on Mg2+, Zn2+, Mn2+, Ca2+ cannot replace Mg2+