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Literature summary for 7.6.2.4 extracted from

  • Geillon, F.; Gondcaille, C.; Raas, Q.; Dias, A.M.M.; Pecqueur, D.; Truntzer, C.; Lucchi, G.; Ducoroy, P.; Falson, P.; Savary, S.; Trompier, D.
    Peroxisomal ATP-binding cassette transporters form mainly tetramers (2017), J. Biol. Chem., 292, 6965-6977 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 7.6.2.4

Cloned(Commentary)

Cloned (Comment) Organism
gene ABCD1, recombinant expression of the enzyme in COS-7 cells and in Rattus norvegicus H4IIEC3 hepatoma cell model Mus musculus
recombinant expression of EGFP-tagged enzyme in COS-7 cells and in Rattus norvegicus H4IIEC3 hepatoma cell model, microglial BV-2 cells expressing ABCD2 chimeric homodimers (ABCD2-ABCD2 plus ABCD2-ABCD2-EGFP) also show tetramer formation of the proteins Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
peroxisomal membrane ABCD1 and its homologue ABCD2 exist mainly as homotetramers in the peroxisomal membrane, quaternary structure of the ABCD1 protein in the peroxisomal membrane, overview Mus musculus 5778
-
peroxisomal membrane ABCD1 and its homologue ABCD2 exist mainly as homotetramers in the peroxisomal membrane, quaternary structure of the ABCD2 protein in the peroxisomal membrane, overview Mus musculus 5778
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Mus musculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
82000
-
 monomer of ABCD1 protein, sucrose density sedimentation centrifugation analysis Mus musculus
111000
-
 monomer of EGFP-tagged ABCD2 protein, sucrose density sedimentation centrifugation analysis Mus musculus
215000 415000 tetramer of ABCD1 protein, sucrose density sedimentation centrifugation analysis Mus musculus
270000 515000 tetramer of EGFP-tagged ABCD2 protein, sucrose density sedimentation centrifugation analysis Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + H2O + fatty acyl CoA[side 1] Mus musculus
-
 ADP + phosphate + fatty acyl CoA[side 2]
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus P48410
-
-
Mus musculus Q61285
-
-

Source Tissue

Source Tissue Comment Organism Textmining
BV-2 cell
-
 Mus musculus
-
BV-2 cell the microglial cells expressing ABCD2 chimeric homodimers (ABCD2-ABCD2 plus ABCD2-ABCD2-EGFP) also show tetramer formation of the proteins Mus musculus
-
microglial cell line
-
 Mus musculus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + H2O + fatty acyl CoA[side 1]
-
 Mus musculus ADP + phosphate + fatty acyl CoA[side 2]
-
 ?

Subunits

Subunits Comment Organism
heterotetramer quaternary structure of the ABCD1 protein in the peroxisomal membrane, overview Mus musculus
heterotetramer quaternary structure of the ABCD2 protein in the peroxisomal membrane, overview Mus musculus
homodimer quaternary structure of the ABCD1 protein in the peroxisomal membrane, overview Mus musculus
homodimer quaternary structure of the ABCD2 protein in the peroxisomal membrane, overview Mus musculus
homotetramer quaternary structure of the ABCD1 protein in the peroxisomal membrane, overview Mus musculus
homotetramer quaternary structure of the ABCD2 protein in the peroxisomal membrane, overview Mus musculus
More both transporters, ABCD1 and ABCD2, exist as both homo- and heterotetramers, with a predominance of homotetramers. In addition to tetramers, some larger molecular ABCD assemblies are also found but represent only a minor fraction Mus musculus
More both transporters, ABCD1 and ABCD2, exist as both homo- and heterotetramers, with a predominance of homotetramers. In addition to tetramers, some larger molecular ABCD assemblies are also found but represented only a minor fraction Mus musculus

Synonyms

Synonyms Comment Organism
ABCD1
-
 Mus musculus
ABCD2
-
 Mus musculus

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Mus musculus

General Information

General Information Comment Organism
evolution the ABCD1 transporter belongs to the D subfamily of the ATP-binding cassette (ABC)2 transporter family and is encoded by the ABCD1 gene Mus musculus
evolution the ABCD2 transporter belongs to the D subfamily of the ATP-binding cassette (ABC)2 transporter family and is encoded by the ABCD1 gene Mus musculus
additional information absence of interaction between solubilized ABCD1 and ABCD2 within supradimeric assemblies, but ABCD1 interacts with ABCD2 in the BV-2 microglial cell line and in cell lysates, interaction analysis. ABCD1 and ABCD2 tetrameric assemblies remain unchanged during the catalytic cycle of the transporters Mus musculus
physiological function ABCD1 and its homologue ABCD2 are peroxisomal ATP-binding cassette (ABC) half-transporters of fatty acyl-CoAs with both distinct and overlapping substrate specificities Mus musculus